Morphological and anatomical properties of Veronica crista-galli Steven

Nigar Hidayet Pashayeva, T. Suleymanov, Y. Kerimov, E. Gasimov, F. Rzayev
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Abstract

The aim of this work was to study of diagnostic signs of the morphological and anatomical structure of Veronica crista-galli Steven. from the flora of Azerbaijan. Materials and methods. The samples for research were collected during their flowering time in June 2018, in the Ismailli region of the Republic of Azerbaijan. Plant samples were fixed in a solution made in 0.1 M phosphate buffer (pH=7.4), containing 2.5 % glutar-aldehyde, 2.5 % paraformal-aldehyde and 0.1 % picric acid. In the next stage was the preparation of block and their filling in Araldite – Epon according to the TEM method. Results. The leaf is simple, lower part is short-petiolate and upper is sessile. The surface, on both sides of the leaf, is reliefly, and 7–8 conductive veins are clearly visible. The lower and upper sides of the leaf, and also margin, are strewn with multicellular hairs. The calyx of the flower consists of two sepals which grown together at the base, covered with simple multicellular hairs. The stalk in is a long filiform. The corolla of flower consists of 4 petals which grown together at the base and 2 stamens attached to the tube of the corolla. On the epidermis, cells with sinuous and bead-like walls, numerous stomata of the stavrocytic type, capitate hairs are visible. From the cross section of the leaf, it is visible that palisade tissue at the upper and sponge tissue at the bottom. Conclusions. As a result of morphological and anatomical studies, it was revealed that diagnostic signs of plant raw material can be: Present of multicellular hairs on the leaf blade; The location of the capsule between the sepals; Stavrocytic type of the stoma structure; The bead-like walls of the epidermis; Capitate hairs on the epidermis; Sepals covered by hairs. The established anatomical diagnostic features can be used for the drafting of the normative document on the plant raw materials and for identification of plant raw material of Veronica crista-galli
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维罗妮卡的形态解剖学特征
本研究的目的是研究维罗妮卡(Veronica crista-galli Steven)的形态和解剖结构的诊断标志。来自阿塞拜疆的植物群。材料和方法。用于研究的样本是在2018年6月阿塞拜疆共和国伊斯梅尔地区的开花期间收集的。将植物样品固定在0.1 M磷酸盐缓冲液(pH=7.4)中,该缓冲液含有2.5%戊醛、2.5%多聚甲醛和0.1%苦味酸。下一阶段是用透射电镜法制备块体并在alaldite - Epon中进行填充。结果。单叶,下部短叶柄,上部无梗。叶片两侧的表面呈浮雕状,7-8条导电脉清晰可见。叶的下部和上部,以及边缘,散布着多细胞毛。花的花萼由基部生长在一起的两片萼片组成,被简单的多细胞毛所覆盖。茎为长丝状。花冠由基部生长的4片花瓣和附着在花冠筒上的2个雄蕊组成。在表皮上,可见具有弯曲和珠状细胞壁的细胞,大量的星胞型气孔,头状毛。从叶片的横截面上,可以看到上部的栅栏组织和底部的海绵组织。结论。通过形态学和解剖学的研究,揭示了植物原料的诊断标志:叶片上有多细胞毛;蒴果在萼片之间的位置;气孔结构为星胞型;表皮的珠状壁;头状毛在表皮上;萼片被毛覆盖。所建立的解剖诊断特征可用于植物原料的规范性文件的起草,也可用于维罗妮卡植物原料的鉴定
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