The aim of the research was to compare the shape, viability, metabolic and proliferative activity of mesenchymal stromal cells (MSCs) during cultivation in hydrogels and macroporous scaffolds. Materials and methods. Human adipose tissue MSCs were isolated from lipoaspirates of healthy adult donors after obtaining informed consent. Hydrogels were obtained from platelet-poor human blood plasma and alginate polymer, cross-linked with calcium ions in microspheres. Macroporous scaffolds were prepared from plasma by the cryotropic gelation method. Morphology and viability of cells within carriers were assessed using vital dyes. Metabolic and proliferative activity of MSCs was studied by the Alamar Blue test on the 1st, 3rd and 7th day of 3D culturing. Results. Three-dimensional blood plasma scaffolds had a branched pore structure with a size sufficient for cell proliferation and migration. When plasma proteins were cross-linked with L-cysteine, almost all MSCs were viable, attached to the pore surface, spread and proliferated, filling carrier cavities. In plasma hydrogels, MSCs occupied spaces and acquired a fibroblast-like morphology, maintaining viability. In alginate microspheres, MSCs were uniform distributed throughout the gel volume, kept their spherical shape, but had high viability. The highest metabolic activity of MSCs was observed in macroporous scaffolds, the lowest one in alginate microspheres. During cultivation, the activity of cells in macroporous scaffolds and plasma hydrogels increased significantly, which indirectly indicated the proliferation processes. Conclusions. Properties of MSCs during 3D cultivation significantly depend on the microenvironment: in blood plasma carriers, cells acquire a fibroblast-like morphology and proliferate, while in alginate microspheres, they remain spherical and do not proliferate.
{"title":"Modulation of mesenchymal stromal cells properties by the microenvironment in 3D culture","authors":"Oleksandr Petrenko, Olena Rogulska, Natalia Trufanova, Oleh Trufanov, Oleksandra Hubenia, Olena Revenko, Daria Cherkashina","doi":"10.15587/2519-8025.2023.288082","DOIUrl":"https://doi.org/10.15587/2519-8025.2023.288082","url":null,"abstract":"The aim of the research was to compare the shape, viability, metabolic and proliferative activity of mesenchymal stromal cells (MSCs) during cultivation in hydrogels and macroporous scaffolds. Materials and methods. Human adipose tissue MSCs were isolated from lipoaspirates of healthy adult donors after obtaining informed consent. Hydrogels were obtained from platelet-poor human blood plasma and alginate polymer, cross-linked with calcium ions in microspheres. Macroporous scaffolds were prepared from plasma by the cryotropic gelation method. Morphology and viability of cells within carriers were assessed using vital dyes. Metabolic and proliferative activity of MSCs was studied by the Alamar Blue test on the 1st, 3rd and 7th day of 3D culturing. Results. Three-dimensional blood plasma scaffolds had a branched pore structure with a size sufficient for cell proliferation and migration. When plasma proteins were cross-linked with L-cysteine, almost all MSCs were viable, attached to the pore surface, spread and proliferated, filling carrier cavities. In plasma hydrogels, MSCs occupied spaces and acquired a fibroblast-like morphology, maintaining viability. In alginate microspheres, MSCs were uniform distributed throughout the gel volume, kept their spherical shape, but had high viability. The highest metabolic activity of MSCs was observed in macroporous scaffolds, the lowest one in alginate microspheres. During cultivation, the activity of cells in macroporous scaffolds and plasma hydrogels increased significantly, which indirectly indicated the proliferation processes. Conclusions. Properties of MSCs during 3D cultivation significantly depend on the microenvironment: in blood plasma carriers, cells acquire a fibroblast-like morphology and proliferate, while in alginate microspheres, they remain spherical and do not proliferate.","PeriodicalId":21636,"journal":{"name":"ScienceRise: Biological Science","volume":"46 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135040246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-30DOI: 10.15587/2519-8025.2023.287424
Ksenia Havrylenko
Aim of the research was to investigate the impact of agricultural activity on the concentration of Alternaria spores.
Materials and methods. The study was carried out at the Department of Medical Biology, Parasitology and Genetics of the ZSMPhU. Samples were collected using a 7-day volumetric sampler of the Hirst type, using the volumetric method. Samples were identified under a light microscope, and spore identification and counting were limited to genus levels. The relationship between seasonal Alternaria spore levels and harvest rates was analysed using Pearson's correlation method. The effect of meteorological conditions and agricultural activity on the daily concentration of Alternaria was analysed using stepwise correlation based on logarithmically transformed daily average spore counts. Classical leave-one-out cross-validation (LOOCV) was used to estimate the mean square error (MSE), associated with this model and Bayesian information criterion (BIC) was used to assess its accuracy.
Results. Seasonal characteristics of Alternaria spores and agricultural activity in Zaporizhzhia and Dnipro regions were analysed. The connection of some seasonal and daily indicators with harvesting rates and meteorological conditions was determined. Two models with 5 and 9 parameters were found that best explain the dynamics of Alternaria spores.
Conclusions. The most significant parameters positively correlated with Alternaria spore levels were temperature, pressure, westerly wind and wheat yield; relative humidity was negatively correlated
{"title":"The influence of agricultural land on the level of airborne Alternaria spores","authors":"Ksenia Havrylenko","doi":"10.15587/2519-8025.2023.287424","DOIUrl":"https://doi.org/10.15587/2519-8025.2023.287424","url":null,"abstract":"Aim of the research was to investigate the impact of agricultural activity on the concentration of Alternaria spores.
 Materials and methods. The study was carried out at the Department of Medical Biology, Parasitology and Genetics of the ZSMPhU. Samples were collected using a 7-day volumetric sampler of the Hirst type, using the volumetric method. Samples were identified under a light microscope, and spore identification and counting were limited to genus levels. The relationship between seasonal Alternaria spore levels and harvest rates was analysed using Pearson's correlation method. The effect of meteorological conditions and agricultural activity on the daily concentration of Alternaria was analysed using stepwise correlation based on logarithmically transformed daily average spore counts. Classical leave-one-out cross-validation (LOOCV) was used to estimate the mean square error (MSE), associated with this model and Bayesian information criterion (BIC) was used to assess its accuracy.
 Results. Seasonal characteristics of Alternaria spores and agricultural activity in Zaporizhzhia and Dnipro regions were analysed. The connection of some seasonal and daily indicators with harvesting rates and meteorological conditions was determined. Two models with 5 and 9 parameters were found that best explain the dynamics of Alternaria spores.
 Conclusions. The most significant parameters positively correlated with Alternaria spore levels were temperature, pressure, westerly wind and wheat yield; relative humidity was negatively correlated","PeriodicalId":21636,"journal":{"name":"ScienceRise: Biological Science","volume":"60 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135041275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-30DOI: 10.15587/2519-8025.2023.288228
Andriy Berezovskiy, Tetiana Fotina, Yevheniia Vashchyk, Olga Bobrytska, Nataliia Seliukova, Sergiy Shtrygol’, Andriy Zakhariev, Ruslan Dubin
The aim: study of the effectiveness of environmentally safe means «Vetoks-1000», «Sumerian silver» for the prevention of pseudomonosis of poultry embryos, associated with bacteriosis.
Materials and methods. The effectiveness of the preparations «VetOks-1000», «Sumerian silver» in relation to E. coli, P. aeruginos, S. aureus, S. typhimurium was determined by the method of researching antimicrobial activity on test objects: galvanized iron, wooden bars (painted and unpainted), red brick and plaster cut-outs, 10 x 10 cm, and a hatching egg.
The effectiveness of «VetOks-1000», «Sumerian silver» solutions compared to formaldehyde for disinfection of hatching eggs and incubation cabinets for the purpose of preventing pseudomonosis of poultry embryos was carried out in the production conditions of the hatchery, where P. aeruginosa, E. coli, S. аureus, were periodically isolated from asphyxiated embryos and from the carcasses of chicks of the first 10 days of life by comparing the hatching rate of chicks in the experimental and control groups.
Results. It was established that the «Sumerian silver» had an antimicrobial effect against the causative agents of pseudomonosis and the main bacterioses of poultry in concentrations of 1–3 %, and the drug «VetOx-1000»was effective in concentrations of 0.024 %–0.03 % after exposure for 1-4 hours in depending on the type of surface.
The number of conditioned young birds, obtained with the use of environmentally safe means «VetOks-1000», «Sumerian silver», was higher by (1.1-1.7) % compared to the number of young birds in the control group using formalin.
Conclusions. The proposed method of prevention of associated pseudomonosis of poultry embryos by rotation of environmentally safe means in established bactericidal concentrations (3 % «Sumerian silver» solution and 0.03 % «VetOks 1000» solution) ensures a 1.1–1.7 % higher hatching of young birds compared to control using formalin
{"title":"Effectiveness of environmentally safe products «VetOks-1000», «Sumerian silver» for the prevention of pseudomonosis of poultry embryos associated with bacteriosis","authors":"Andriy Berezovskiy, Tetiana Fotina, Yevheniia Vashchyk, Olga Bobrytska, Nataliia Seliukova, Sergiy Shtrygol’, Andriy Zakhariev, Ruslan Dubin","doi":"10.15587/2519-8025.2023.288228","DOIUrl":"https://doi.org/10.15587/2519-8025.2023.288228","url":null,"abstract":"The aim: study of the effectiveness of environmentally safe means «Vetoks-1000», «Sumerian silver» for the prevention of pseudomonosis of poultry embryos, associated with bacteriosis.
 Materials and methods. The effectiveness of the preparations «VetOks-1000», «Sumerian silver» in relation to E. coli, P. aeruginos, S. aureus, S. typhimurium was determined by the method of researching antimicrobial activity on test objects: galvanized iron, wooden bars (painted and unpainted), red brick and plaster cut-outs, 10 x 10 cm, and a hatching egg.
 The effectiveness of «VetOks-1000», «Sumerian silver» solutions compared to formaldehyde for disinfection of hatching eggs and incubation cabinets for the purpose of preventing pseudomonosis of poultry embryos was carried out in the production conditions of the hatchery, where P. aeruginosa, E. coli, S. аureus, were periodically isolated from asphyxiated embryos and from the carcasses of chicks of the first 10 days of life by comparing the hatching rate of chicks in the experimental and control groups.
 Results. It was established that the «Sumerian silver» had an antimicrobial effect against the causative agents of pseudomonosis and the main bacterioses of poultry in concentrations of 1–3 %, and the drug «VetOx-1000»was effective in concentrations of 0.024 %–0.03 % after exposure for 1-4 hours in depending on the type of surface.
 The number of conditioned young birds, obtained with the use of environmentally safe means «VetOks-1000», «Sumerian silver», was higher by (1.1-1.7) % compared to the number of young birds in the control group using formalin.
 Conclusions. The proposed method of prevention of associated pseudomonosis of poultry embryos by rotation of environmentally safe means in established bactericidal concentrations (3 % «Sumerian silver» solution and 0.03 % «VetOks 1000» solution) ensures a 1.1–1.7 % higher hatching of young birds compared to control using formalin","PeriodicalId":21636,"journal":{"name":"ScienceRise: Biological Science","volume":"130 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135040245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-30DOI: 10.15587/2519-8025.2023.286148
Olga Filiptsova, Olga Naboka, Svitlana Bobro, Olexander Bashura, Vira Myrhorod, Yuliia Osypenko, Liudmyla Petrovska
The aim. The aim of the study was to analyze aspects related to a healthy diet and lifestyle, more specifically to the consumption of plant-based food among Ukrainian preteens and teenagers aged 10 to 17 years.
Materials and methods. 231 individuals aged 10 to 17 participated in the study, 85 of them were boys, 146 were girls, all of them were residents of Kharkiv city at the time of participation in the study. The respondents were presented with a questionnaire regarding their attitude to a healthy lifestyle, in particular, rational nutrition. The χ2 test was used to analyze the qualitative data.
Results and discussion. In the work, it was found that more than half of the respondents led only a partially healthy lifestyle, 60.3 % and 54.1 % among girls and boys, respectively. Only 11.6 % and 18.8 % of girls and boys followed the diet, although the majority of preteens and teenagers did not skip breakfast (80.8 % of girls and 89.4 % of boys). Unfortunately, the vast majority of subjects (58.9 % of girls and 56.5 % of boys) sometimes indulged in "harmful food". Also, the majority of preteens and teenagers consumed food between principal meals (83.6 % of girls and 72.9 % of boys), which, taking into account current ideas, should be considered an alarming trend. The main motivation for choosing food among preteens and teenagers was their own food tastes and family traditions, while food advertising was taken into account by just over 1 % of preteens and teenagers. Self-assessment of the health state shows that only 2/3 to ¾ of preteens and teenagers had no complaints about their health. No sex differences were observed for all the indicated characteristics. The most popular vegetable product among Ukrainian preteens and teenagers was potato, it was consumed by more than ¾ of preteens and teenagers, its popularity grew with age, which can be considered a negative trend (fast food). The least popular plant-based product among preteens and teenagers was seeds, consumed by only 19.9 % of girls and 8.2 % of boys. It was the only plant-based product, for which a statistically significant difference in consumption was found (2.4 times more common among girls than among boys). With age, the frequency of coffee consumption as an "adult drink" increased among preteens and teenagers, while a "leap" in the prevalence of its consumption can be seen when passing from 14-15 years to 16-17 years. Therefore, most of the results obtained should be considered as concerning ones.
Conclusions. The consumption of plant-based food among Ukrainian preteens and teenagers is almost not associated with sex, while the consumption of some types of plant-based food is more closely related to the age of preteens and teenagers, which may indicate the continuation of the process of forming food habits in this ontogenesis period. The practical value of the work lies in potential corrective recommendations from doctors, valeologists, nutritionists, psychologists and
{"title":"Dietary preferences and analysis of the \"plant-based food basket\" among Ukrainian preteens and teenagers","authors":"Olga Filiptsova, Olga Naboka, Svitlana Bobro, Olexander Bashura, Vira Myrhorod, Yuliia Osypenko, Liudmyla Petrovska","doi":"10.15587/2519-8025.2023.286148","DOIUrl":"https://doi.org/10.15587/2519-8025.2023.286148","url":null,"abstract":"The aim. The aim of the study was to analyze aspects related to a healthy diet and lifestyle, more specifically to the consumption of plant-based food among Ukrainian preteens and teenagers aged 10 to 17 years.
 Materials and methods. 231 individuals aged 10 to 17 participated in the study, 85 of them were boys, 146 were girls, all of them were residents of Kharkiv city at the time of participation in the study. The respondents were presented with a questionnaire regarding their attitude to a healthy lifestyle, in particular, rational nutrition. The χ2 test was used to analyze the qualitative data.
 Results and discussion. In the work, it was found that more than half of the respondents led only a partially healthy lifestyle, 60.3 % and 54.1 % among girls and boys, respectively. Only 11.6 % and 18.8 % of girls and boys followed the diet, although the majority of preteens and teenagers did not skip breakfast (80.8 % of girls and 89.4 % of boys). Unfortunately, the vast majority of subjects (58.9 % of girls and 56.5 % of boys) sometimes indulged in \"harmful food\". Also, the majority of preteens and teenagers consumed food between principal meals (83.6 % of girls and 72.9 % of boys), which, taking into account current ideas, should be considered an alarming trend. The main motivation for choosing food among preteens and teenagers was their own food tastes and family traditions, while food advertising was taken into account by just over 1 % of preteens and teenagers. Self-assessment of the health state shows that only 2/3 to ¾ of preteens and teenagers had no complaints about their health. No sex differences were observed for all the indicated characteristics. The most popular vegetable product among Ukrainian preteens and teenagers was potato, it was consumed by more than ¾ of preteens and teenagers, its popularity grew with age, which can be considered a negative trend (fast food). The least popular plant-based product among preteens and teenagers was seeds, consumed by only 19.9 % of girls and 8.2 % of boys. It was the only plant-based product, for which a statistically significant difference in consumption was found (2.4 times more common among girls than among boys). With age, the frequency of coffee consumption as an \"adult drink\" increased among preteens and teenagers, while a \"leap\" in the prevalence of its consumption can be seen when passing from 14-15 years to 16-17 years. Therefore, most of the results obtained should be considered as concerning ones.
 Conclusions. The consumption of plant-based food among Ukrainian preteens and teenagers is almost not associated with sex, while the consumption of some types of plant-based food is more closely related to the age of preteens and teenagers, which may indicate the continuation of the process of forming food habits in this ontogenesis period. The practical value of the work lies in potential corrective recommendations from doctors, valeologists, nutritionists, psychologists and","PeriodicalId":21636,"journal":{"name":"ScienceRise: Biological Science","volume":"34 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135040247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-30DOI: 10.15587/2519-8025.2023.288227
Anatoly Bozhkov, Svitlana Bilovetska
Aim: To test the hypothesis about the possible role of vitamin A in normalizing the functional activity of the liver with Cu-induced fibrosis by increasing the regeneration process.
Materials and methods: Experiments were conducted on 20 sexually mature male Wistar rats, which were divided into 4 groups: a control group that was not exposed to copper sulfate and vitamin A, a group that was at the initial stage of liver fibrosis, which was provided by three consecutive administrations of copper sulfate at a dose of 1 mg/100 g of weight (one series of injections), a group that was at the stage of intensive development of fibrosis (F2), which was carried out by two consecutive series of copper sulfate injections with an interval of 3 days between injections, and a group that received vitamin A three times daily in a dose of 300 IU/100 g of weight between two series of intoxication. Body weight dynamics, relative liver weight, histological changes in liver tissues and the number of binuclear hepatocytes were determined.
Results: It has been found that animals with Cu-induced liver fibrosis did not gain or lose body weight, and the introduction of vitamin A ensured the restoration of body weight growth, and they slightly lagged behind the control group. In animals with liver fibrosis that received vitamin A, the relative weight of the liver was slightly increased and there were 2 times more binuclear hepatocytes. The structural organization of the liver tissue changed to a minor extent, and to the greatest extent there was an increase in the thickness of the Glisson’s capsule, in which immunocompetent cells were incorporated.
Conclusions: Vitamin A contributed to the normalization of liver function against the background of the development of fibrosis. The mechanism of normalization can be ensured due to an increase in the number of binuclear hepatocytes, a slight increase in the relative weight of the liver, and was accompanied by an increase in the thickness of the Glisson’s capsule, in which immunocompetent cells were incorporated
{"title":"Vitamin A accelerates the process of liver regeneration in the initial stages of Сu - induced fibrosis","authors":"Anatoly Bozhkov, Svitlana Bilovetska","doi":"10.15587/2519-8025.2023.288227","DOIUrl":"https://doi.org/10.15587/2519-8025.2023.288227","url":null,"abstract":"Aim: To test the hypothesis about the possible role of vitamin A in normalizing the functional activity of the liver with Cu-induced fibrosis by increasing the regeneration process.
 Materials and methods: Experiments were conducted on 20 sexually mature male Wistar rats, which were divided into 4 groups: a control group that was not exposed to copper sulfate and vitamin A, a group that was at the initial stage of liver fibrosis, which was provided by three consecutive administrations of copper sulfate at a dose of 1 mg/100 g of weight (one series of injections), a group that was at the stage of intensive development of fibrosis (F2), which was carried out by two consecutive series of copper sulfate injections with an interval of 3 days between injections, and a group that received vitamin A three times daily in a dose of 300 IU/100 g of weight between two series of intoxication. Body weight dynamics, relative liver weight, histological changes in liver tissues and the number of binuclear hepatocytes were determined.
 Results: It has been found that animals with Cu-induced liver fibrosis did not gain or lose body weight, and the introduction of vitamin A ensured the restoration of body weight growth, and they slightly lagged behind the control group. In animals with liver fibrosis that received vitamin A, the relative weight of the liver was slightly increased and there were 2 times more binuclear hepatocytes. The structural organization of the liver tissue changed to a minor extent, and to the greatest extent there was an increase in the thickness of the Glisson’s capsule, in which immunocompetent cells were incorporated.
 Conclusions: Vitamin A contributed to the normalization of liver function against the background of the development of fibrosis. The mechanism of normalization can be ensured due to an increase in the number of binuclear hepatocytes, a slight increase in the relative weight of the liver, and was accompanied by an increase in the thickness of the Glisson’s capsule, in which immunocompetent cells were incorporated","PeriodicalId":21636,"journal":{"name":"ScienceRise: Biological Science","volume":"38 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135040244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-30DOI: 10.15587/2519-8025.2023.288085
Uliana Semak, Mylenka Myroslava
Thermal power plant (TPP) facilities are considered as one of the major reasons for environmental pollution. Ash and slag dumps as a special construction for storage of combustion wastes of TPPs are recognized as sources of heavy metals (HMs) contamination for surrounding ecosystems. The present study is the first report of analyzing HMs contamination of the ash and slag dumps of the Burshtyn TPP.
The aim of the study is to estimate the content of HMs in the technogenic substrates of ash and slag dumps and investigate soil-plant interactions through analyzing potential of HMs accumulation in the leaves of native dominant woody species.
Materials and methods of research. Soil sampling was carried out in the period of July 2021 at previously determined points. The most common woody species (Populus tremula L., Betula pendula Roth., Salix caprea L.) were selected for testing of HMs accumulation abilities. Samples of plants and soil were subjected to an atomic absorption spectrometer for being analyzed for heavy metals: Cd, Zn, Ni, Cu, Pb, Mn and Fe.
Results of research and discussion. The results showed that the substrates of ash and slag dumps of the Burshtyn TPP were mainly contaminated by lead, copper and cadmium. All tested species concentrated high amounts of magnesium, iron, zinc and low concentration of cadmium. Bioaccumulation factor reflected the highest abilities of accumulation of zinc in all tested species and low level of bioaccumulation of cadmium. The highest index of biochemical activity showed Betula pendula. Salix caprea were found as a promising species for remediation due to intensive accumulation of such elements like cadmium, lead, copper, zinc and nickel.
Conclusions and prospects for further research. We consider plant organisms particularly useful for analyzing HMs accumulation as they can provide a cost-effective and long-term approach for bioindication and monitoring HMs pollution. Moreover, vegetation covers could be used for remediation of HMs contaminated sites
{"title":"Accumulation of heavy metals in leaves of tree species on the ash and slag dumps of the Burshtyn Thermal Power Plant","authors":"Uliana Semak, Mylenka Myroslava","doi":"10.15587/2519-8025.2023.288085","DOIUrl":"https://doi.org/10.15587/2519-8025.2023.288085","url":null,"abstract":"Thermal power plant (TPP) facilities are considered as one of the major reasons for environmental pollution. Ash and slag dumps as a special construction for storage of combustion wastes of TPPs are recognized as sources of heavy metals (HMs) contamination for surrounding ecosystems. The present study is the first report of analyzing HMs contamination of the ash and slag dumps of the Burshtyn TPP.
 The aim of the study is to estimate the content of HMs in the technogenic substrates of ash and slag dumps and investigate soil-plant interactions through analyzing potential of HMs accumulation in the leaves of native dominant woody species.
 Materials and methods of research. Soil sampling was carried out in the period of July 2021 at previously determined points. The most common woody species (Populus tremula L., Betula pendula Roth., Salix caprea L.) were selected for testing of HMs accumulation abilities. Samples of plants and soil were subjected to an atomic absorption spectrometer for being analyzed for heavy metals: Cd, Zn, Ni, Cu, Pb, Mn and Fe.
 Results of research and discussion. The results showed that the substrates of ash and slag dumps of the Burshtyn TPP were mainly contaminated by lead, copper and cadmium. All tested species concentrated high amounts of magnesium, iron, zinc and low concentration of cadmium. Bioaccumulation factor reflected the highest abilities of accumulation of zinc in all tested species and low level of bioaccumulation of cadmium. The highest index of biochemical activity showed Betula pendula. Salix caprea were found as a promising species for remediation due to intensive accumulation of such elements like cadmium, lead, copper, zinc and nickel.
 Conclusions and prospects for further research. We consider plant organisms particularly useful for analyzing HMs accumulation as they can provide a cost-effective and long-term approach for bioindication and monitoring HMs pollution. Moreover, vegetation covers could be used for remediation of HMs contaminated sites","PeriodicalId":21636,"journal":{"name":"ScienceRise: Biological Science","volume":"38 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135041276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-30DOI: 10.15587/2519-8025.2023.285648
Olga Naboka, Alla Kotvitska, Alina Volkova, Oksana Tkachenko, Yuliya Voronina-Tuzovskykh, Olga Filiptsova, Inna Pasynchuk
The work is devoted to the search for new biologically active substances in a series of alkylamides of dihydroquinoline-3-carboxylic acid. Analgesic properties of 30 newly synthesized substances were studied, which made it possible to identify the leader compound (provisional name alkylcarb) and recommend its effectiveness for further research as an antispasmodic agent for pain relief. It has been established experimentally, that the substance alkylcarb exhibits concentration-dependent vasodilatory properties in vitro on segments of the thoracic aorta of rats. The data, obtained in the work, justify the prospect of using the leader substance as a new analgesic and antispasmodic drug. The research expands and deepens knowledge about the pharmacological properties of alkylamide derivatives of dihydroquinoline-3-carboxylic acid.
The aim of the work was to conduct screening studies to establish the analgesic activity of a newly synthesized series of chemical compounds among alkylamides of dihydroquinoline-3-carboxylic acid and to study the myotropic spasmolytic activity of the leader substance.
Materials and methods. Analgesic properties of alkylamides of dihydroquinoline-3-carboxylic acid were studied on outbred white mice in the "acetic acid convulsions" test. During the experiment, the animals were treated in accordance with the International Principles of the European Convention for the Protection of Vertebrate Animals Used for Experiments and Other Scientific Purposes (Strasbourg, March 18, 1986). The studied substances were administered intragastrically to experimental animals at a dose of 10 mg/kg in the form of a finely dispersed aqueous suspension, stabilized with Tween-80. Voltaren at a dose of 8 mg/kg, recommended for preclinical studies, and analgin at a dose of 50 mg/kg were chosen as reference drugs.
The studies of the contractile activity of smooth muscle vessels were carried out on segments of the thoracic aorta of rats of both sexes weighing 180-200 g. The studies of dilator reactions were carried out against the background of preliminary contraction with phenylephrine at a concentration of 10-6 mol/l. The antispasmodic efficiency of the new compound was determined in comparison with the classic antispasmodic drotaverine.
The statistical processing of the results was carried out using the package of statistical analysis of electronic spreadsheets Exel, with the help of the program "Statgraphics Plus v. 3.0.” and the standard package of statistical programs "Statistica, V. 6.0". We used the Student's test, a non-parametric analog of univariate variance analysis - the Kruskal-Wallis test, and the Mann-Whitney test. Differences were considered statistically significant at p<0.05.
Results. The study of the analgesic activity of substances АO1-АO30 in the "acetic acid convulsion" test in mice showed that a compound АO26 (provisional name alkylcarb) has the most pronounced analgesic activity when administered
本工作致力于在一系列二氢喹啉-3-羧酸烷基酰胺中寻找新的生物活性物质。研究了30种新合成物质的镇痛特性，从而鉴定了先导化合物(暂定名为烷基碳水化合物)，并推荐了其作为镇痛药的进一步研究效果。实验证实，烷基碳水化合物在体外对大鼠胸主动脉段具有浓度依赖性的血管扩张特性。这项工作中获得的数据证明了使用先导物质作为一种新的镇痛和抗痉挛药物的前景是正确的。该研究拓展和深化了对二氢喹啉-3-羧酸烷基酰胺衍生物药理性质的认识。
本工作的目的是进行筛选研究，以确定新合成的二氢喹啉-3-羧酸烷基酰胺系列化合物的镇痛活性，并研究其先导物质的肌解痉活性。
材料和方法。采用醋酸惊厥试验研究了二氢喹啉-3-羧酸烷基胺类化合物对远交系小白鼠的镇痛作用。在实验过程中，所有动物都按照《保护用于实验和其他科学目的的脊椎动物的欧洲公约国际原则》(斯特拉斯堡，1986年3月18日)进行处理。实验动物以10 mg/kg的剂量以细分散的水悬浮液形式灌胃所研究的物质，用吐温-80稳定。参考药物选用临床前研究推荐剂量为8mg /kg的伏他仑和50mg /kg的安良酮。对体重180 ~ 200 g的雌雄大鼠的胸主动脉段进行了平滑肌血管收缩活动的研究。在浓度为10-6 mol/l的苯肾上腺素初步收缩的背景下，进行了扩张反应的研究。新化合物的抗痉挛效果与经典的抗痉挛药牛膝草胺比较。使用电子表格统计分析软件包Exel，借助Statgraphics Plus v. 3.0程序对结果进行统计处理。以及统计程序的标准软件包“Statistica, V. 6.0”。我们使用了学生检验，一种单变量方差分析的非参数模拟- Kruskal-Wallis检验和Mann-Whitney检验。p<0.05;
结果。在小鼠“醋酸惊厥”试验中对物质АO1-АO30的镇痛活性的研究表明，一种化合物АO26(暂称烷基碳水化合物)在灌胃时具有最明显的镇痛活性。在“醋酸惊厥”试验中，烷基碳水化合物(10 mg/kg，每os)可能会减少由醋酸引起的惊厥次数。该化合物的活性水平与双氯芬酸的活性相当(8mg /kg，每os)，并超过安良酮(50mg /kg，每os)。在大鼠胸主动脉离体片段模型上进行的体外实验中，烷基碳水化合物对血管痉挛的缓解作用与对照药氯他弗林的水平相当。结论。目前，临床使用的止痛药不符合有效性和安全性的要求，寻找新的高效非阿片类镇痛药是现代药理学亟待解决的问题。为此，近年来，国家药科大学的科学家们在二氢喹啉-3-羧酸烷基酰胺类化合物中，积极寻找具有抗菌、抗炎、解热作用的新型高效物质。
{"title":"Study of analgesic and myotropic spasmolytic activity of alkylcarb","authors":"Olga Naboka, Alla Kotvitska, Alina Volkova, Oksana Tkachenko, Yuliya Voronina-Tuzovskykh, Olga Filiptsova, Inna Pasynchuk","doi":"10.15587/2519-8025.2023.285648","DOIUrl":"https://doi.org/10.15587/2519-8025.2023.285648","url":null,"abstract":"The work is devoted to the search for new biologically active substances in a series of alkylamides of dihydroquinoline-3-carboxylic acid. Analgesic properties of 30 newly synthesized substances were studied, which made it possible to identify the leader compound (provisional name alkylcarb) and recommend its effectiveness for further research as an antispasmodic agent for pain relief. It has been established experimentally, that the substance alkylcarb exhibits concentration-dependent vasodilatory properties in vitro on segments of the thoracic aorta of rats. The data, obtained in the work, justify the prospect of using the leader substance as a new analgesic and antispasmodic drug. The research expands and deepens knowledge about the pharmacological properties of alkylamide derivatives of dihydroquinoline-3-carboxylic acid.
 The aim of the work was to conduct screening studies to establish the analgesic activity of a newly synthesized series of chemical compounds among alkylamides of dihydroquinoline-3-carboxylic acid and to study the myotropic spasmolytic activity of the leader substance.
 Materials and methods. Analgesic properties of alkylamides of dihydroquinoline-3-carboxylic acid were studied on outbred white mice in the \"acetic acid convulsions\" test. During the experiment, the animals were treated in accordance with the International Principles of the European Convention for the Protection of Vertebrate Animals Used for Experiments and Other Scientific Purposes (Strasbourg, March 18, 1986). The studied substances were administered intragastrically to experimental animals at a dose of 10 mg/kg in the form of a finely dispersed aqueous suspension, stabilized with Tween-80. Voltaren at a dose of 8 mg/kg, recommended for preclinical studies, and analgin at a dose of 50 mg/kg were chosen as reference drugs.
 The studies of the contractile activity of smooth muscle vessels were carried out on segments of the thoracic aorta of rats of both sexes weighing 180-200 g. The studies of dilator reactions were carried out against the background of preliminary contraction with phenylephrine at a concentration of 10-6 mol/l. The antispasmodic efficiency of the new compound was determined in comparison with the classic antispasmodic drotaverine.
 The statistical processing of the results was carried out using the package of statistical analysis of electronic spreadsheets Exel, with the help of the program \"Statgraphics Plus v. 3.0.” and the standard package of statistical programs \"Statistica, V. 6.0\". We used the Student's test, a non-parametric analog of univariate variance analysis - the Kruskal-Wallis test, and the Mann-Whitney test. Differences were considered statistically significant at p<0.05.
 Results. The study of the analgesic activity of substances АO1-АO30 in the \"acetic acid convulsion\" test in mice showed that a compound АO26 (provisional name alkylcarb) has the most pronounced analgesic activity when administered ","PeriodicalId":21636,"journal":{"name":"ScienceRise: Biological Science","volume":"138 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136365309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The aim of the study was to study the gastroprotective properties of the dry extract of garden cabbage in the model of chronic acetic ulcer in rats.
Materials and methods. Chronic ulcerative lesions of the gastric mucosa of rats were modeled by introducing 0.05 ml of a 30 % solution of acetic acid into the subserous layer of the stomach wall. The dry extract of garden cabbage in a conditionally therapeutic dose of 50 mg/kg and reference drugs omeprazole and altan were administered intragastrically for 10 days, starting from the second day of the experiment. When choosing the duration of the course of treatment, we proceeded from the data that the effectiveness of the antiulcer action in the clinic is assessed after 10-12 days of treatment. The percentage of animals with ulcers in the group, the state of the gastric mucosa were evaluated, the ulcer index and antiulcer activity were calculated. To elucidate the possible mechanism of action of the extract, we studied its effect on synthetic processes in terms of the content of RNA and DNA in homogenates of the gastric mucosa by the spectrophotometric method by reaction with perchloric acid.
Results. On the model of stomach damage, caused by acetic acid, a pronounced antiulcer activity of the extract (54.26) was established at the level of omeprazole (54.62). The maximum antiulcer activity (72.75) was revealed when using the combination of the extract with omeprazole, which, in terms of ulcerative effect, is superior to monotherapy with the extract, omeprazole and altan (35.08).
Reparative regeneration is a component of an adaptive tissue reaction; under conditions of violation of the reparative potencies of the mucosa, chronic diseases of the gastrointestinal tract develop. Since the final goal of antiulcer therapy is the healing of the ulcer, and the basis of this process is reparative regeneration, the reparative properties of the extract have been studied. Under the influence of cabbage extract, the content of RNA and DNA in the mucosa probably increased by 2.8 and 2.4 times, respectively, at the same level with omeprazole. If possible, to activate the synthetic processes of DEGC and omeprazole, altan significantly prevailed.
The most pronounced effect of enhancing reparative processes was established with the combined use of the extract and omeprazole, which turned out to be a significant increase in the content of RNA and DNA in the gastric mucosa by 3.2 and 2.9 times compared with the control.
Conclusions. On the model of chronic damage to the stomach of rats, cabbage extract showed an antiulcer effect, the severity of which was not inferior to the comparison drug, the proton pump inhibitor omeprazole, and significantly exceeded the phytopreparation - altan. The combined use of the extract and omeprazole showed the highest preventive effect of preventing the negative effects of acetic acid on the gastric mucosa. One of the mechanisms of the gastroprotective action
本研究旨在探讨甘蓝干提取物对大鼠慢性乙酸溃疡模型的胃保护作用。材料和方法。将0.05 ml 30%醋酸溶液注入胃壁浆膜下层，建立大鼠胃黏膜慢性溃疡病变模型。从实验第2天开始，以条件治疗剂量50 mg/kg的大白菜干提取物和对照药物奥美拉唑、阿勒坦灌胃10 d。在选择疗程时，我们从临床抗溃疡作用的有效性数据出发，在治疗10-12天后进行评估。测定各组动物溃疡发生率、胃粘膜状态，计算溃疡指数和抗溃疡活性。为了阐明其可能的作用机制，我们通过与高氯酸反应，用分光光度法研究了其对胃粘膜匀浆中RNA和DNA含量的影响。结果。在醋酸致胃损伤模型上，其抗溃疡活性(54.26)达到奥美拉唑水平(54.62)。与奥美拉唑合用抗溃疡活性最高(72.75)，溃疡效果优于与奥美拉唑、阿勒坦单用(35.08)。修复再生是适应性组织反应的一个组成部分;在违反粘膜的修复能力的条件下，胃肠道的慢性疾病发展。由于抗溃疡治疗的最终目的是溃疡的愈合，而这一过程的基础是修复再生，因此研究了提取物的修复特性。在奥美拉唑作用下，大白菜提取物可使小鼠粘膜中RNA和DNA含量分别提高2.8倍和2.4倍。在可能的情况下，为了激活DEGC和奥美拉唑的合成过程，altan明显占上风。
与对照组相比，该提取物与奥美拉唑联合使用对修复过程的促进作用最为显著，胃粘膜中RNA和DNA的含量显著增加了3.2倍和2.9倍。
结论。在大鼠胃慢性损伤模型上，白菜提取物显示出抗溃疡作用，其程度不逊于对照药质子泵抑制剂奥美拉唑，且明显超过植物修复-阿勒坦。提取物与奥美拉唑联用对预防醋酸对胃粘膜的不良影响效果最好。其胃保护作用的机制之一是其增强胃粘膜修复过程的能力。研究结果表明，白菜提取物的抗溃疡作用有待进一步研究，以期将其应用于消化性溃疡的综合治疗
{"title":"Study of the antiulcer activity of garden cabbage extract on the chronic acetic ulcer model in rats","authors":"Nadiia Kononenko, Valentina Chikitkina, Larysa Karabut, Olena Matviichuk, Olha Vislous","doi":"10.15587/2519-8025.2023.285891","DOIUrl":"https://doi.org/10.15587/2519-8025.2023.285891","url":null,"abstract":"The aim of the study was to study the gastroprotective properties of the dry extract of garden cabbage in the model of chronic acetic ulcer in rats.
 Materials and methods. Chronic ulcerative lesions of the gastric mucosa of rats were modeled by introducing 0.05 ml of a 30 % solution of acetic acid into the subserous layer of the stomach wall. The dry extract of garden cabbage in a conditionally therapeutic dose of 50 mg/kg and reference drugs omeprazole and altan were administered intragastrically for 10 days, starting from the second day of the experiment. When choosing the duration of the course of treatment, we proceeded from the data that the effectiveness of the antiulcer action in the clinic is assessed after 10-12 days of treatment. The percentage of animals with ulcers in the group, the state of the gastric mucosa were evaluated, the ulcer index and antiulcer activity were calculated. To elucidate the possible mechanism of action of the extract, we studied its effect on synthetic processes in terms of the content of RNA and DNA in homogenates of the gastric mucosa by the spectrophotometric method by reaction with perchloric acid.
 Results. On the model of stomach damage, caused by acetic acid, a pronounced antiulcer activity of the extract (54.26) was established at the level of omeprazole (54.62). The maximum antiulcer activity (72.75) was revealed when using the combination of the extract with omeprazole, which, in terms of ulcerative effect, is superior to monotherapy with the extract, omeprazole and altan (35.08).
 Reparative regeneration is a component of an adaptive tissue reaction; under conditions of violation of the reparative potencies of the mucosa, chronic diseases of the gastrointestinal tract develop. Since the final goal of antiulcer therapy is the healing of the ulcer, and the basis of this process is reparative regeneration, the reparative properties of the extract have been studied. Under the influence of cabbage extract, the content of RNA and DNA in the mucosa probably increased by 2.8 and 2.4 times, respectively, at the same level with omeprazole. If possible, to activate the synthetic processes of DEGC and omeprazole, altan significantly prevailed.
 The most pronounced effect of enhancing reparative processes was established with the combined use of the extract and omeprazole, which turned out to be a significant increase in the content of RNA and DNA in the gastric mucosa by 3.2 and 2.9 times compared with the control.
 Conclusions. On the model of chronic damage to the stomach of rats, cabbage extract showed an antiulcer effect, the severity of which was not inferior to the comparison drug, the proton pump inhibitor omeprazole, and significantly exceeded the phytopreparation - altan. The combined use of the extract and omeprazole showed the highest preventive effect of preventing the negative effects of acetic acid on the gastric mucosa. One of the mechanisms of the gastroprotective action","PeriodicalId":21636,"journal":{"name":"ScienceRise: Biological Science","volume":"3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136365308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The aim: This study aims to determine the diagnostic value of lung ultrasound compared with radiography for respiratory distress in cats.
Materials and methods: The database of the veterinary center was analyzed. 130 animals diagnosed with pulmonary edema were selected. The lungs of sick cats were examined ultrasonographically; The line was counted in 4 anatomical sections on each hemithorax. A site was evaluated as positive when > 3 "B-lines" were detected. Animal treatment protocols were studied to clarify the final diagnosis (reference standard), and the sensitivity and specificity of lung ultrasound and chest X-ray for the diagnosis of pulmonary edema were calculated.
Result: Cats with a final diagnosis of cardiogenic pulmonary edema had a greater number of positive areas on ultrasound than those, in which respiratory distress was caused by non-cardiogenic pulmonary edema. The overall sensitivity and specificity of US for the diagnosis of pulmonary edema were 87 % and 89 %, respectively, and these values were similar to those of chest radiography (85 % and 86 %, respectively). The use of ultrasound led to a false diagnosis of cardiogenic pulmonary edema (ie, a false-positive result) in animals with diffuse interstitial or alveolar changes.
Conclusions: Ultrasound examination of the lungs in cats with respiratory distress syndrome is a promising diagnostic method. Emergency diagnosis of pulmonary edema in cats is difficult, especially in patients with severe shortness of breath, and limits the diagnostic evaluation. Chest x-rays are considered the standard diagnostic test, but the results are sometimes ambiguous and the process of obtaining the x-rays can increase respiratory distress in the animal.
According to the results of the study, it was established, that ultrasound examination of the lungs can be used to differentiate the causes of shortness of breath (cardiogenic and non-cardiogenic) with sufficiently high sensitivity and specificity and less influence of the iatrogenic factor on the development of respiratory distress in cats, compared to chest radiography
{"title":"Ultrasound and x-ray examination at lung edema of domestic cat","authors":"Tetіana Lykholat, Nataliіa Grushanska, Pavlo Sharandak, Vitalii Kostenko, Andrii Rozumniuk","doi":"10.15587/2519-8025.2023.283679","DOIUrl":"https://doi.org/10.15587/2519-8025.2023.283679","url":null,"abstract":"The aim: This study aims to determine the diagnostic value of lung ultrasound compared with radiography for respiratory distress in cats.
 Materials and methods: The database of the veterinary center was analyzed. 130 animals diagnosed with pulmonary edema were selected. The lungs of sick cats were examined ultrasonographically; The line was counted in 4 anatomical sections on each hemithorax. A site was evaluated as positive when > 3 \"B-lines\" were detected. Animal treatment protocols were studied to clarify the final diagnosis (reference standard), and the sensitivity and specificity of lung ultrasound and chest X-ray for the diagnosis of pulmonary edema were calculated.
 Result: Cats with a final diagnosis of cardiogenic pulmonary edema had a greater number of positive areas on ultrasound than those, in which respiratory distress was caused by non-cardiogenic pulmonary edema. The overall sensitivity and specificity of US for the diagnosis of pulmonary edema were 87 % and 89 %, respectively, and these values were similar to those of chest radiography (85 % and 86 %, respectively). The use of ultrasound led to a false diagnosis of cardiogenic pulmonary edema (ie, a false-positive result) in animals with diffuse interstitial or alveolar changes.
 Conclusions: Ultrasound examination of the lungs in cats with respiratory distress syndrome is a promising diagnostic method. Emergency diagnosis of pulmonary edema in cats is difficult, especially in patients with severe shortness of breath, and limits the diagnostic evaluation. Chest x-rays are considered the standard diagnostic test, but the results are sometimes ambiguous and the process of obtaining the x-rays can increase respiratory distress in the animal.
 According to the results of the study, it was established, that ultrasound examination of the lungs can be used to differentiate the causes of shortness of breath (cardiogenic and non-cardiogenic) with sufficiently high sensitivity and specificity and less influence of the iatrogenic factor on the development of respiratory distress in cats, compared to chest radiography","PeriodicalId":21636,"journal":{"name":"ScienceRise: Biological Science","volume":"31 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136365311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The aim: is to study the therapeutic effectiveness of the new veterinary medicinal product Revozyn RTU 400 mg/ml (suspension for injections), manufactured by Eurovet Animal Health B.V. (Netherlands), in the treatment of acute (clinical) and hidden (subclinical) mastitis in cows during lactation.
Materials and methods. The research was conducted on dairy cows of the Simmental breed with a milk productivity of 6000-6500 kg of milk per lactation (F "Pchany-Denkovich", Pchany village, Stryi district, Lviv region). To confirm the diagnosis of "acute mastitis" and to identify cows with hidden (subclinical) mastitis, as well as to establish the effectiveness of the researched drug after treatment, a sample was taken with the California Mastitis Test (CMT), manufactured by Bayer Animal Health GmbH, Germany. For bacteriological research, milk samples were taken from the affected quarters of the mammary gland (1 sample from each cow) in compliance with generally accepted sanitary rules. The sensitivity of bacterial isolates to the drug was determined by diffusion in agar using standard discs with benzylpenicillin.
Results. A clinical examination of 127 Simmental dairy cows of different periods of lactation was carried out. During the clinical-diagnostic examination of animals, 8 cows with clinically pronounced, acute course of mastitis were found. According to the nature of the exudate in 3 cows, mastitis was serous, in 5 cows it was purulent-catarrhal. For serous mastitis, the CM test gave a positive result – thickening, the milk solution looks like a gel. In case of purulent catarrh, a sample with the CMT gave a strongly positive result – the mixture thickened, the gel took on a certain shape and became very viscous. The CMT revealed 12 cows with suspected latent mastitis. Representatives of opportunistic microflora – Streptococcus agalactiae, Staphylococcus aureus, and Escherichia coli bacteria were isolated and identified from mastitis milk. The summarized results of the clinical trial of the drugs Revozyn RTU 400 mg/ml and Procillin® 30 % on cows suffering from the clinical form of mastitis were as follows. On the 10th day of the experiment, a milk sample with the California mastitis test showed that all milk samples from cows of both groups (with the exception of one milk sample from a cow from the control group) gave a negative result (the solution remained liquid, blue or gray and homogeneous, without clots). Taking into account the results of the CMT and the number of somatic cells in the milk after the therapy, the therapeutic effectiveness of the veterinary medicinal product Revozyn RTU 400 mg/ml in the treatment of various forms of mastitis in lactating cows was 100 %, and the one of the comparative drug Procillin® 30 % was 83.3 % .
Conclusions. A clinical study of the veterinary medicinal product Revozyn RTU 400 mg/ml, suspension for injections, manufactured by Eurovet Animal Health B.V. (Netherlands) established its effectiv
{"title":"Effectiveness of a new means of etiotropic therapy of mastits in cows during the lactation period","authors":"Taras Stetsko, Larysa Ostrovska, Yevhen Kostyschyn, Orest Katsaraba, Lidiia-Mariia Kostyshyn, Dmytro Morozenko","doi":"10.15587/2519-8025.2023.284845","DOIUrl":"https://doi.org/10.15587/2519-8025.2023.284845","url":null,"abstract":"The aim: is to study the therapeutic effectiveness of the new veterinary medicinal product Revozyn RTU 400 mg/ml (suspension for injections), manufactured by Eurovet Animal Health B.V. (Netherlands), in the treatment of acute (clinical) and hidden (subclinical) mastitis in cows during lactation.
 Materials and methods. The research was conducted on dairy cows of the Simmental breed with a milk productivity of 6000-6500 kg of milk per lactation (F \"Pchany-Denkovich\", Pchany village, Stryi district, Lviv region). To confirm the diagnosis of \"acute mastitis\" and to identify cows with hidden (subclinical) mastitis, as well as to establish the effectiveness of the researched drug after treatment, a sample was taken with the California Mastitis Test (CMT), manufactured by Bayer Animal Health GmbH, Germany. For bacteriological research, milk samples were taken from the affected quarters of the mammary gland (1 sample from each cow) in compliance with generally accepted sanitary rules. The sensitivity of bacterial isolates to the drug was determined by diffusion in agar using standard discs with benzylpenicillin.
 Results. A clinical examination of 127 Simmental dairy cows of different periods of lactation was carried out. During the clinical-diagnostic examination of animals, 8 cows with clinically pronounced, acute course of mastitis were found. According to the nature of the exudate in 3 cows, mastitis was serous, in 5 cows it was purulent-catarrhal. For serous mastitis, the CM test gave a positive result – thickening, the milk solution looks like a gel. In case of purulent catarrh, a sample with the CMT gave a strongly positive result – the mixture thickened, the gel took on a certain shape and became very viscous. The CMT revealed 12 cows with suspected latent mastitis. Representatives of opportunistic microflora – Streptococcus agalactiae, Staphylococcus aureus, and Escherichia coli bacteria were isolated and identified from mastitis milk. The summarized results of the clinical trial of the drugs Revozyn RTU 400 mg/ml and Procillin® 30 % on cows suffering from the clinical form of mastitis were as follows. On the 10th day of the experiment, a milk sample with the California mastitis test showed that all milk samples from cows of both groups (with the exception of one milk sample from a cow from the control group) gave a negative result (the solution remained liquid, blue or gray and homogeneous, without clots). Taking into account the results of the CMT and the number of somatic cells in the milk after the therapy, the therapeutic effectiveness of the veterinary medicinal product Revozyn RTU 400 mg/ml in the treatment of various forms of mastitis in lactating cows was 100 %, and the one of the comparative drug Procillin® 30 % was 83.3 % .
 Conclusions. A clinical study of the veterinary medicinal product Revozyn RTU 400 mg/ml, suspension for injections, manufactured by Eurovet Animal Health B.V. (Netherlands) established its effectiv","PeriodicalId":21636,"journal":{"name":"ScienceRise: Biological Science","volume":"4 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136365307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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