Identification of novel haplotype of a cyst nematode resistance gene, GmSNAP18 in soybean [Glycine max (L.) Merr.]

IF 1 4区生物学 Q3 PLANT SCIENCES Indian Journal of Genetics and Plant Breeding Pub Date : 2020-09-22 DOI:10.31742/IJGPB.80.3.5

I. Choi, P. Basnet, Hana Yoo, N. Roy, R. Ramekar, Kyong-Cheul Park

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Abstract

Soybean cyst nematode (SCN) is one of the most damaging pest of soybean. Discovery and characterization of the genes involved in SCN resistance are important in soybean breeding. Soluble NSF attachment protein (SNAP) genes are related to SCN resistance in soybean. SNAP genes include five gene families, and 2 haplotypes of exons 6 and 9 of SNAP18 are considered resistant to the SCN. In present study the haplotypes of GmSNAP18 were surveyed and chacterized in a total of 60 diverse soybean genotypes including Korean cultivars, landraces, and wild-types. The target region of exons 6 and 9 in GmSNAP18 region was amplified and sequenced to examine nucleotide variation. Characterization of 5 haplotypes identified in present study for the GmSNAP18 gene revealed two haplotypes as resistant, 1 as susceptible and two as novel. A total of twelve genotypes showed resistant haplotypes, and 45 cultivars were found susceptible. Interestingly, the two novel haplotypes were present in 3 soybean lines. The information provided here about the haplotypic variation of GmSNAP18 gene can be further explored for soybean breeding to develop resistant varieties.

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大豆抗囊线虫基因GmSNAP18新单倍型的鉴定[j]稳定)

大豆囊线虫是危害大豆最严重的害虫之一。大豆抗SCN基因的发现和鉴定在大豆育种中具有重要意义。可溶性NSF附着蛋白(SNAP)基因与大豆抗SCN有关。SNAP基因包括5个基因家族，SNAP18外显子6和9的2个单倍型被认为对SCN具有抗性。本研究对包括韩国品种、地方品种和野生型在内的60种大豆基因型进行了GmSNAP18单倍型的调查和鉴定。扩增GmSNAP18区域外显子6和9的靶区并测序以检测核苷酸变异。本研究对GmSNAP18基因鉴定的5个单倍型进行了鉴定，结果显示2个为抗性单倍型，1个为易感单倍型，2个为新颖单倍型。共有12个基因型表现出抗性单倍型，45个品种表现出易感。有趣的是，这两种新的单倍型存在于3个大豆系中。本研究提供的GmSNAP18基因单倍型变异信息，可为大豆育种开发抗性品种提供参考。

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来源期刊

Indian Journal of Genetics and Plant Breeding PLANT SCIENCES-

CiteScore

1.80

自引率

10.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Advance the cause of genetics and plant breeding and to encourage and promote study and research in these disciplines in the service of agriculture; to disseminate the knowledge of genetics and plant breeding; provide facilities for association and conference among students of genetics and plant breeding and for encouragement of close relationship between them and those in the related sciences; advocate policies in the interest of the nation in the field of genetics and plant breeding, and facilitate international cooperation in the field of genetics and plant breeding.