High-throughput screening of blood samples based on structured illumination on-chip imaging

Abstract

We demonstrate a high-throughput fluorescent on-chip imaging platform, using structured illumination that can rapidly screen large volumes of scattering media such as undiluted whole blood samples (e.g., ~ 0.3-0.7 mL) for detection of fluorescent micro-objects at low concentrations (e.g., ≤ 50-100 particles/mL). This imaging modality might especially be useful for rare cell applications involving whole blood samples, such as detection of circulating tumor cells.