Acute Toxicity and the Effects of Mangifera indica on Serum IL-6, and IFN-γ in Breast Cancer-Induced Albino Rats

Abstract

Background: Breast cancer is an uncontrolled growth of breast tissue, which develops in cells lining the milk ducts and lobules, it’s the most common neoplasm in the female. Breast cancer has been declared a universal disaster as it is expected to nearly triple between 2020 and 2030, as most available drugs have not shown any desirable outcome. Aims/Objective: This research aimed to evaluate the acute toxicity and effects of M. indica on serum IL-6, and IFN-γ in cancer-induced albino rats. Materials/Methods: Mangifera indica was subjected to plant identification/authentication and extractions, the acute toxicity was determined using Lorke's method. They are 6 groups of 4 rats each. The groups are normal, positive controls, Ascorbic acids, 500mg, 1000mg and 1500mg M. indica groups. All the groups were induced with 65 mg/kg-1 b.w. of 7,12 Dimethylbenzene-(α) anthracene (DMBA), except Group I and observed for 14 days, before treatment with 100mg of AA (Group III), and 500mg, 1000mg, and 1500mg of extracts (Groups IV- VI) respectively. The rats were sacrificed, 24 hours. after the last treatment. Results: The results of acute toxicity study of the extracts in both phase 1 and 2, has shown no signs of behavioural changes and mortality in all the experimental animals. This has proven that methanolic extracts of M. indica is safe. There was a significant down-regulation of serum IL-6, and INF-γ expressions (P>0.005). Conclusion: This research indicated that M. indica extract is safe and possesses anti-tumor, and immunomodulatory effects, it may be used for breast cancer management.