Genotoxic Effects of Karamenderes River (Canakkale/Turkey) Water Samples on Vicia faba L. Plant.

Meric Can Bayrak, C. Aki
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Abstract

In this research the effects of Karamenderes River’s water s amples which collected from six different locations have been used for determining the genoto xic potential by the meaning of mitotic index analysis in Vicia faba L . In this research root tip t est were used for mitotic index measuring. For comparing the effects of six different location s of Karamenderes River’s water samples. Water samples have been applied on four weeks old V. faba p lantlets. After 24 and 48 hours exposure time root tip test were realized. All experiments were carried out under controlled conditions in the plant growth chamber. According to our research results, it seems that water samples which collected from si x different locations have been effec ted mit otic index differently. When we compare with control group, decreasing in mitotic index in V. faba have b een shown especially in station five and six after 48 hours exposure time. After 24 hours of application de creasing has been observed as 43,99% and 50,30% respectively when compared with the c ontrol group. After 48 hours of application decreasing has been observed as 47,11% and 51,07% resp ectively when compared with the control group. According to our research results, it was found that mitosis is affected at different levels in V. faba plantlets depending on the water quality and applicat ion time in the six stations of Karamenderes River. Keywords: Vicia faba , Karamenderes River, Mitotic DOI : 10.7176/ 7-04-04
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Karamenderes河(Canakkale/土耳其)水样对蚕豆植物的遗传毒性效应
本研究采用有丝分裂指数法测定了卡拉蒙德勒斯河6个不同地点的水样对蚕豆的基因毒性势的影响。本研究采用根尖试验测定有丝分裂指数。为了比较Karamenderes河六个不同地点的水样的影响。水样应用于四周龄蚕豆苗。暴露24小时和48小时后进行根尖试验。所有实验均在植物生长室内受控条件下进行。根据我们的研究结果,从六个不同地点采集的水样对生物指数的影响似乎不同。与对照组相比,暴露48 h后蚕豆有丝分裂指数明显下降,尤其是第5和第6工位。施用24 h后,与对照组相比,分别减少43.99%和50.30%。施用48h后,与对照组相比,分别下降了47.11%和51.07%。根据我们的研究结果,在Karamenderes河6个站点,不同水质和施药时间对蚕豆苗有丝分裂有不同程度的影响。关键词:蚕豆,Karamenderes河,有丝分裂DOI: 10.7176/ 7-04-04
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