Emergence of Storm Resistant Mechanisms in Pseudomonas aeruginosa Isolated from Burn Patients Hospitalized in Ghotbeddin Shirazi Burn Hospital

Abstract

Introduction: Pseudomonas aeruginosa is a bacterium has capability of presenting different policies against antimicrobial agents. These capacities make this bacterium as one of the most important cause of nosocomial infection especially among burned patients. Methods: In this cross sectional study, total of 253 hospitalized burned patients (During September 2013 to November 2014) in Ghotbeddin Shirazi were evaluated for P. aeruginosa. Confirmed isolates tested for Metallo-β-lactamase (MBLs) producing and related genes bla IMPI, II , bla SPM , bla KHM , bla VIM , efflux pump activity, and OprD mutations. Results: From total of 56 (22%) P. aeruginosa isolates, 32 (57.14%) were MBLs while in genetic evaluation, only bla IMPII, bla KHM and bla VIM genes were detected. While 52 (92.8%) isolates had MexAB-OprM gene but 44(78.6%) isolates had efflux pump activity. In OprD sequencing evaluation it has been proved that the most imipenem resistance isolates have mutations in this related gene. Based on the results it has been proved that the circulating P. aeruginosa isolates in Shiraz burn center are armed with the most potential resistant mechanisms. Conclusion: According to that the most isolates have the potential of express different mechanisms of resistance, it is highly recommended to evaluate the circulating infectious agents periodically and determine their resistant algorithm to control them before being resistant and being dominant in health centers.