Comparative proteome analysis in hot pepper (Capsicum annuum L.) after space flight

Abstract

Hot pepper (Capsicum annuum L.) is an important cropall over the world. To explore and identify differentially expressedproteins of hot pepper after space flight, three space-induced mutants(Y1, Y2 and Y3), which obtained new traits after space flightcompared with their control lines (W1 and W2), were analyzed usingcomparative proteome analysis. In this study, leaf morphologicalcharacteristics of five kinds of hot pepper variations were evaluatedby scanning electron microscopy (SEM) and transmission electronmicroscopy (TEM). Results showed that obvious changes of cellularstructure were seen in space induced mutants. Thirty nine out ofthe 255 differentially expressed proteins were successfully sequenced.Among these 39 proteins, 31 were homologous with known proteins,and the others were set as a hypothetical protein. All identifiedproteins were further classified into six groups including proteinmetabolism, energy metabolism and photosynthesis. In conclusion,these findings will benefit for the research of mutagenic mechanismsat the level of proteomics, and provide an effective way for new hotpepper breeding.