Study of Bone Marrow Cells During First Three Months Old Rats

Abstract

Haematopoiesis is very complicated process of formation, development, and differentiation of bone marrow cells and its evaluation is very valuable diagnostic tool for the veterinary practitioner. So, the aim of study is to evaluate the healthy bone marrow cytology during first three months old. Ninety pups were involved throughout the study according to study design. Growing pups were kept with their moms till the weaning day, then divided into three main groups (1, 2, and 3 months old). After reaching the appropriate study age, bone marrow samples were obtained, and bone marrow smears were carried out and stained with MGGS. The results revealed presence of all types of haematopoietic cells and there are increase in different erythroid progenitor and myeloid progenitor with the age progress. Furthermore, the smears showed different cell line production with different differentiation stages of both erythroid and myeloid cells. In conclusion the bone marrow cells differentiation and proliferation are related to age progress independently. Haematopoietic cells differentiation and proliferation are regulated by bone marrow microenvironment. Myeloid cells are directly related to age progress.