The Longevity of Sexed Sperm after Sexing with Several Combination of Bovine Serum Albumin (BSA) Concentration

Abstract

The aim of the research was to evaluate the longevity of sexed sperm of local ram after sexing with different combination of Bovine Serum Albumin (BSA) concentration in chilled and post thawed sexed sperm. The object of the research was 10 ejaculated semen from 3 years old local ram. The method was a Completely Randomize Design (CRD) with four treatments combination of BSA concentration on upper layer and bottom layer (T1: 3% and 6%, T2: 4% and 8%, T3: 5% and 10%, dan T4: 6% and 12%), each treatment was repeated 10 times. Result showed that there is significant difference (P<0.05) in sperm longevity both in chilled and post thawed sexed sperm. Sperm longevity in chilled sexed sperm on of upper layer in T1, T2, T3 and T4 were 229.60±16.13, 231.90±10.27, 265.40±15.01, and 196.60±19.21 hours, respectively, as well as on bottom layer in T1, T2, T3 and T4 were 207.70±31.18, 207.90±31.47, 239.10±30.82 and 181.60±31.81 hours, respectively. Sperm longevity in post thawed sexed sperm on upper layer in T1, T2, T3 and T4 were 142.80±2.68, 142.20±1.64, 147.00±3.00, and 142.20±1.64 hours, respectively, and then on bottom layer were 121.80±2.68, 120.60±3.29, 125.40±3.29 and 118.80±1.64 hours, respectively. In conclusion, the combination of BSA concentration of 5% and 10% at upper and bottom layer resulting longest sperm longevity both in chilled and post thawed sexed sperm.