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Construction, Cloning, and Overexpression of Staphylococcal Enterotoxin B Gene Synthetic (SEBsyn) in pET-28a(+): Pre-development Bacterial-Toxin Therapy for Cancer 葡萄球菌肠毒素B基因合成(SEBsyn)在pET-28a(+)中的构建、克隆和过表达:发育前细菌毒素治疗癌症
Pub Date : 2023-01-17 DOI: 10.2991/absr.k.220406.065
Achmad Rodiansyah, M. Tan, H. Nugrahapraja
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引用次数: 1
Proceedings of the International Conference of Tropical Studies and Its Applications (ICTROPS 2022) 国际热带研究及其应用会议论文集(ICTROPS 2022)
Pub Date : 2023-01-01 DOI: 10.2991/978-94-6463-180-7
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引用次数: 0
Growth Assessment of Broiler Chickens Given Bitter Leaves (Vernonia amygdalina) as Phyto-additive, Potentially Antimicrobial Agents of Lipids and Amino Acids 苦叶作为植物添加剂、潜在的脂类和氨基酸抗菌剂对肉鸡生长的影响
Pub Date : 2022-01-01 DOI: 10.2991/absr.k.220207.005
J. S. Mandey, M. Sompie, F. Wolayan
This research was conducted to evaluate the growth of broiler chickens given bitter leaves (Vernonia amygdalina) in the drink as phytoadditive, potentially antimicrobial agents of lipids and amino acids. Amino acids of bitter leaves were analyzed by HPLC method, the fatty acid and fat content of bitter leaves were analyzed by A.O.A.C. Official Methods 2012. Antibacterial analysis of bitter leaves was prepared in three concentrations (2.5%, 5% and 10%), and analyzed with disc diffusion method. A total of 200 D.O.C. broiler chicks were treated under a standard broiler management program until 7 days of age. The experiment was conducted as 4x5 completely randomized arrangement. The experiment lasted 28 days from. Bitter leaves juice was blended and given to broilers through drinking water in four kinds of treatment: 0, 10, 20 and 30 ml/L drinking water. All chickens were fed diet contain 70% commercial feed, 27% broken corn and 3% coconut oil and given ad libitum. Results showed that the bitter leaves contain high linolenic, linoleic and palmitic fatty acid, and phenylalanine, serine, isoleucine, glycine and arginine amino acids. Bitter leaves showed the power of antibacterial activity for S. aureus. Bitter leaves as feed additives in drinking water of broiler caused feed intake and carcass percentage were non significantly different. Final weight, WG, SGR, GE, FCR were highly significantly increased. Abdominal fat was highly significantly decreased, and there was no effect on the giblet. It can be concluded that bitter leaf can be used as phytoaddive in broiler diet.
本研究以苦杏仁叶为植物添加剂,对肉仔鸡的生长进行了评价。苦杏仁叶是一种潜在的抗脂质和氨基酸剂。采用高效液相色谱法分析苦叶中氨基酸含量,采用A.O.A.C.官方方法2012分析苦叶中脂肪酸和脂肪含量。制备苦叶3种浓度(2.5%、5%和10%)的抑菌分析,采用圆盘扩散法进行分析。选取200只D.O.C.肉鸡雏鸡,按标准肉鸡管理方案处理至7日龄。实验采用4x5完全随机安排。试验期28 d。将苦叶汁配制成0、10、20、30 ml/L 4种处理方式,分别饲喂肉鸡。饲粮中添加70%的商品饲料、27%的碎玉米和3%的椰子油,并随意饲喂。结果表明,苦叶中含有较高的亚麻酸、亚油酸和棕榈酸脂肪酸,以及苯丙氨酸、丝氨酸、异亮氨酸、甘氨酸和精氨酸等氨基酸。苦叶对金黄色葡萄球菌具有较强的抑菌活性。在肉鸡饮水中添加苦叶对采食量和胴体率无显著影响。末重、WG、SGR、GE、FCR均极显著提高。腹部脂肪极显著减少,对赤霉素无影响。由此可见,苦叶可作为肉仔鸡饲粮中的植物添加剂。
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引用次数: 1
Gentamicin Resistance on Escherichia coli Isolated from Cats 猫源大肠杆菌对庆大霉素的耐药性研究
Pub Date : 2022-01-01 DOI: 10.2991/absr.k.220309.090
Yamin Yaddi, Safika, F. Pasaribu
Antibiotic resistance is currently an important problem in the field of animal and human health. The main triggering factor for the incidence of bacterial resistance is wisdom in the use of antibiotics. Handling cases of bacterial infections in cats still make antibiotics be the main choice. One of the antibiotics that is still used in cases of bacterial infection in cats is gentamicin. The use of this antibiotic is oriented towards treating ear infections (otitis). Bacteria that are often found as the cause of otitis cases come from Gram-positive and Gram-negative bacteria. Resistance to bacteria in the ear cavity can occur through uncontrolled use of antibiotics or interactions with other bacteria that are resistant. Interactions between bacteria that cause the transfer of genetic material carrying resistant traits have been widely reported in Gram-negative bacteria through plasmid intermediaries. Body licking behavior in cats allows interactions between bacteria from different habitats, especially Escherichia coli from the digestive tract. Escherichia coli has been reported to have developed resistance in various animals and humans. The study aimed to measure the resistance level of Escherichia coli isolated from cats to gentamicin. The method used in this research is disk diffusion Kirby-Bauer. The results of this study show that 56 samples of cat rectal swab are isolated from Escherichia coli and confirmed by biochemical tests. There are 11 samples (19.64%) showing resistance and 45 (80.36%) susceptible samples. These results indicate that gentamicin can still be one of the antibiotics choice in the treatment of cases of Escherichia coli infection in both the ear cavity and digestive tract..
抗生素耐药性是当前动物和人类健康领域的一个重要问题。细菌耐药发生的主要触发因素是使用抗生素的智慧。处理猫的细菌感染病例仍然使抗生素成为主要选择。庆大霉素是目前仍用于治疗猫细菌感染的抗生素之一。使用这种抗生素是为了治疗耳部感染(中耳炎)。引起中耳炎的细菌通常来自革兰氏阳性菌和革兰氏阴性菌。对耳腔内细菌的耐药性可以通过不受控制地使用抗生素或与其他耐药细菌相互作用而发生。细菌之间的相互作用导致携带抗性性状的遗传物质通过质粒媒介在革兰氏阴性细菌中转移已被广泛报道。猫的舔身体行为允许来自不同栖息地的细菌之间的相互作用,特别是来自消化道的大肠杆菌。据报道,大肠杆菌在各种动物和人类中产生了耐药性。这项研究旨在测量从猫身上分离出的大肠杆菌对庆大霉素的耐药性水平。本研究采用的方法是圆盘扩散Kirby-Bauer法。本研究结果表明,56份猫直肠拭子样品分离出大肠杆菌,并经生化试验证实。耐药11份(19.64%),敏感45份(80.36%)。这些结果表明庆大霉素仍可作为治疗耳腔和消化道大肠杆菌感染病例的首选抗生素之一。
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引用次数: 0
The Effect of Nano Zinc Dietary Supplementation on Growth Performance of KUB Chickens 饲粮中添加纳米锌对KUB鸡生长性能的影响
Pub Date : 2022-01-01 DOI: 10.2991/absr.k.220207.018
N. D. Larasati, B. Ariyadi, N. D. Dono
Current trial was intended to study the effects of nano zinc dietary supplementation on the growth performance of KUB Chickens. Two hundred KUB chicks were distributed randomly into five treatment levels with four replications, and ten chicks in each replicate pen. The dietary nano zinc treatments were: basal diet without nano zinc supplementation (NZ-0) and a basal diet supplementation with nanoparticle zinc oxide at concentrations of 15 ppm (NZ-1), 30 ppm (NZ2), 45 ppm (NZ-3), or 60 ppm (NZ-4). The observed variables were body weight gain, feed intake, and feed conversion ratio (FCR), based on the 10 weeks rearing period. The pooled data were analyzed statistically with one way ANOVA and followed subsequently with Duncan’s test for the data with significant difference. Results indicated that dietary supplementation of 60 ppm nano zinc increased body weight gain (P<0.01) and feed intake (P<0.05), and reduced FCR (P<0.05). Taken together, our data indicated that the growth performance of the KUB chickens improved when the diet were supplemented with 60 ppm nanoparticle zinc
本试验旨在研究饲粮中添加纳米锌对KUB鸡生长性能的影响。试验将200只KUB雏鸡随机分为5个处理水平,每4个重复,每个重复栏10只鸡。饲粮纳米锌处理为:在基础饲粮中不添加纳米锌(NZ-0)和在基础饲粮中添加浓度为15 ppm (nz1)、30 ppm (NZ2)、45 ppm (NZ-3)或60 ppm (NZ-4)的纳米氧化锌。观察的变量为体重增重、采食量和饲料系数(FCR),以10周饲养期为基础。合并后的数据采用单因素方差分析进行统计学分析,对有显著差异的数据采用Duncan检验。结果表明,饲粮中添加60 ppm纳米锌可提高猪体增重(P<0.01)和采食量(P<0.05),降低饲料转化率(P<0.05)。综上所述,我们的数据表明,当饲粮中添加60 ppm纳米颗粒锌时,KUB鸡的生长性能得到改善
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引用次数: 0
Effects of Concentration and Soaking Duration of Shallot Extract on Yield and Growth of Sweet Potato (Ipomoea batatas L.) 葱浸膏浓度和浸泡时间对甘薯产量和生长的影响
Pub Date : 2022-01-01 DOI: 10.2991/978-94-6463-086-2_3
Sipa Nurul Laely, H. H. Nafi'ah, S. Maesyaroh, Jajang Supriatna
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引用次数: 0
Small-scale Fisheries Management Strategy on the Eastern Coast of the Bangka Regency, Indonesia 印度尼西亚邦加摄政东海岸的小规模渔业管理战略
Pub Date : 2022-01-01 DOI: 10.2991/978-94-6463-086-2_80
E. Bidayani, Y. Mardyani, Kurniawan, S. Aisyah, Fahri Setiawan
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引用次数: 0
Effect of Condensed Tannin and Myristic Acid in Corn Straw-Based Complete Feeds on NH3 Concentration and Microbial Protein Synthesis 玉米秸秆全料中浓缩单宁和肉豆蔻酸对NH3浓度和微生物蛋白合成的影响
Pub Date : 2022-01-01 DOI: 10.2991/absr.k.220401.007
S. Chuzaemi, M. Mashudi, P. Ndaru, A. Huda, Eko Agus Siswoyo
This study aimed to determine the effect of condensed tannin (CT) and myristic acid in corn straw-based complete feed on NH 3 concentration and in vitro synthesis of microbial protein. The materials used in this study were corn straw, CT, myristic acid, coffee husk rice bran, soybean meal, copra meal, and palm kernel meal. The method used for this research was an experiment in the laboratory using Randomized Block Design (RBD), which consisted of four treatments and three replications. The treatments in this study were T1 (complete feed (40% corn straw + 60% concentrate), T2 (complete feed (40% corn straw + 60% concentrate) + CT 30 g/kg DM + myristic acid 20 g/kg DM), T3 (complete feed (40% corn straw + 60% concentrate) + CT 30 g/kg DM + myristic acid 30 g/kg DM), and T4 (complete feed (40% corn straw + 60% concentrate) + CT 30 g/ kg DM + myristic acid 40 g/kg DM). The results showed that the addition of CT and myristic acid in the complete feed had no significant effect (P>0.05) on NH 3 concentration. It could be concluded that the addition of CT and myristic acid could increase the nutrient content of the feed. Also, the addition level of CT 30 g/kg DM and myristic acid 40 g/kg DM (T4) gave the best result on synthesis protein microbial efficiency and NH 3 concentration.
本试验旨在研究玉米秸秆全料饲料中添加浓缩单宁(CT)和肉豆蔻酸对nh3浓度和体外微生物蛋白合成的影响。本研究使用的材料为玉米秸秆、CT、肉豆蔻酸、咖啡壳米糠、豆粕、椰粕、棕榈仁粕。本研究采用随机区组设计(RBD)的实验室实验方法,包括4个处理和3个重复。试验处理为T1(全饲料(40%玉米秸秆+ 60%精料)、T2(全饲料(40%玉米秸秆+ 60%精料)+ CT 30 g/kg DM +肉豆蔻酸20 g/kg DM)、T3(全饲料(40%玉米秸秆+ 60%精料)+ CT 30 g/kg DM +肉豆蔻酸30 g/kg DM)、T4(全饲料(40%玉米秸秆+ 60%精料)+ CT 30 g/kg DM +肉豆蔻酸40 g/kg DM)。结果表明,在全料中添加CT和肉豆蔻酸对nh3浓度无显著影响(P < 0.05)。由此可见,添加CT和肉豆蔻酸可提高饲料的营养成分含量。此外,添加30 g/kg DM的CT和40 g/kg DM (T4)的肉豆蔻酸对合成蛋白质微生物效率和nh3浓度的影响效果最好。
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引用次数: 2
Models of the Relationships between Egg Dimension and Egg Quality of Alabio Duck 阿拉比鸭蛋尺寸与蛋品质关系模型的研究
Pub Date : 2022-01-01 DOI: 10.2991/absr.k.220309.027
L. Hartati, Nurul Muawanah, Muhammad Riyadhi, D. Biyatmoko, A. Sulaiman, G. Simanungkalit
This research aimed to establish the relationship models between the length and width of Alabio duck (Anas platyrhynchos Borneo) eggs and the weight of eggshells, albumen, and yolk. Data were collected from 500 eggs and were further examined using Pearson’s correlation and regression analysis. The results showed that egg length was positively correlated with eggshell, albumen, and yolk weights (r=0.28, 0.53, 0.52, respectively; P<0.05). These results were lower than the correlation between egg width and eggshell, albumen, and yolk weights (r=0.46, 0.66, 0.46, respectively; P<0.05). Egg length (x1) and egg width (x2) conjointly had a significant relationship with the weight of shell, albumen, and yolk, with a model of y = -2.59 + 0.34x1 + 0.15 x2 (R2=0.23; P<0.05); y = -51.98 + 0.49x1 + 2.06 x2 (R2=0.56; P<0.05); and y = -32.43 + 0.45x1 + 0.63x2 (R2=0.38; P<0.05), respectively. It was concluded that the length and width of Alabio duck eggs offer the potential to estimate albumen and yolk's weight.
本研究旨在建立鸭蛋长、蛋宽与蛋壳、蛋白、蛋黄重量的关系模型。从500个鸡蛋中收集数据,并进一步使用Pearson相关和回归分析进行检验。结果表明:蛋长与蛋壳、蛋白和蛋黄质量呈正相关(r分别为0.28、0.53、0.52);P < 0.05)。这些结果均低于蛋宽与蛋壳、蛋白和蛋黄质量的相关性(r分别为0.46、0.66、0.46;P < 0.05)。蛋长(x1)和蛋宽(x2)与壳重、蛋白重和蛋黄重均显著相关,模型为y = -2.59 + 0.34x1 + 0.15 x2 (R2=0.23;P < 0.05);y = -51.98 + 0.49x1 + 2.06 x2 (R2=0.56;P < 0.05);y = -32.43 + 0.45x1 + 0.63x2 (R2=0.38;分别为P < 0.05)。由此可见,阿拉比鸭蛋的长度和宽度可作为估算蛋中蛋白和蛋黄重量的指标。
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引用次数: 0
Diversity of Eel (Glass Eel) Based on Morphometric Measurements in the Konaweha River, Southeast Sulawesi 基于形态测量的苏拉威西东南部科纳威哈河鳗鲡(玻璃鳗)多样性
Pub Date : 2022-01-01 DOI: 10.2991/absr.k.220309.095
Utama K. Pangerang, S. A. Lawelle, L. Fekri, M. Idris, Jumadi Lolon Marthen
Eel has economic value and nutritional content needed by the body. The demand for eel continues to increase both in fresh and processed forms. The activity of using eel in the waters of the Konaweha River is still very low; this is related to the low understanding of the community regarding the economic value, nutritional content, cultivation process and processed eel. This study aims to determine the diversity of species of glass eel caught in the Konaweha River estuary, Southeast Sulawesi. Identification of the species of glass eel is determined based on the morphometric character of the percentage value of the ratio of the length of the ano-dorsal length (AD) and the total length (TL) AD/TL (%). There were two species of glass eel caught during the study, namely Anguilla bicolor pacific (49.21%) and Anguilla marmorata (50.79%). Counseling related to utilization and management needs to be applied so that eel fish resources can be sustainable and used continuously.
鳗鱼具有经济价值和人体所需的营养成分。对新鲜和加工鳗鱼的需求继续增加。科纳威哈河水域利用鳗鱼的活动仍然很低;这与社区对鳗鱼的经济价值、营养成分、养殖过程和加工过程的了解较低有关。本研究旨在确定在苏拉威西岛东南部科纳威哈河河口捕获的玻璃鳗物种的多样性。玻璃鳗的种类鉴定是根据其无背长(AD)与总长度(TL)之比的形态计量学特征百分比值(AD /TL)确定的。研究期间捕获了两种玻璃鳗,分别是双色太平洋安圭拉(49.21%)和黑鳗(50.79%)。为使鳗鱼资源得到可持续利用和持续利用,需要对利用和管理进行咨询。
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引用次数: 1
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Advances in Biological Sciences Research
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