{"title":"Expression and Visualization of Red Fluorescent Protein (RFP) in Neurospora crassa","authors":"M. Freitag, E. Selker","doi":"10.4148/1941-4765.1124","DOIUrl":null,"url":null,"abstract":"We report the expression of Discosoma red fluorescent protein (RFP) and RFP fusion proteins in Neurospora crassa. RFP was expressed under the control of the Neurospora ccg-1 promoter in transformants with single copies integrated at the his-3 locus by gene targeting. Because this RFP gene, tdimer2(12), contains a 677 bp direct tandem repeat of dsRed, RFP constructs underwent RIP at high frequency in rid strains. Fusion proteins of RFP to the amino terminus of Neurospora heterochromatin protein 1 (HP1) +","PeriodicalId":12490,"journal":{"name":"Fungal Genetics Reports","volume":"25 1","pages":"14-17"},"PeriodicalIF":0.0000,"publicationDate":"2005-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"37","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Fungal Genetics Reports","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.4148/1941-4765.1124","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 37
Abstract
We report the expression of Discosoma red fluorescent protein (RFP) and RFP fusion proteins in Neurospora crassa. RFP was expressed under the control of the Neurospora ccg-1 promoter in transformants with single copies integrated at the his-3 locus by gene targeting. Because this RFP gene, tdimer2(12), contains a 677 bp direct tandem repeat of dsRed, RFP constructs underwent RIP at high frequency in rid strains. Fusion proteins of RFP to the amino terminus of Neurospora heterochromatin protein 1 (HP1) +
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