Kevin McCluskey, Daren Brown, E. Bredeweg, Scott E Baker
{"title":"Predicting the Identities of su(met-2) and met-3 in Neurospora crassa by Genome Resequencing","authors":"Kevin McCluskey, Daren Brown, E. Bredeweg, Scott E Baker","doi":"10.4148/1941-4765.2183","DOIUrl":"https://doi.org/10.4148/1941-4765.2183","url":null,"abstract":"","PeriodicalId":12490,"journal":{"name":"Fungal Genetics Reports","volume":"23 10","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139686492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Many filamentous ascomycete fungi reproduce primarily asexually, with only occasional sexual generations. This can lead to a departure from the 1:1 mating type ratio that is expected in obligate sexual populations. The relaxed selection on sexual traits also can lead to a decrease in the frequency of female fertile strains in field populations, while male fertility does not similarly decrease since male gametes also can serve as asexual spores. Both changes ultimately impact the strength of genetic drift in populations. The frequency of female sterility likely increases with the time since the last generation of sexual reproduction, such that it can be used to estimate the relative frequency of sexual reproduction. Here I provide additional details relevant to Leslie and Klein’s (1996) model of mixed sexual reproduction and vegetative propagation as related to the frequency of female sterility. This includes new or modified equations that allow for simpler calculations of i) two estimates of relative Ne, ii) the expected range of hermaphrodite frequencies during the cycles of mixed reproduction, and iii) the relative frequency of sex. These equations also are included in spreadsheet templates into which researchers can directly enter frequencies computed from their population data to estimate these parameters for their own populations. These resources will make the results of Leslie and Klein (1996) more accessible and should increase the use of this model in evaluating the frequency of sexual reproduction of filamentous fungi.
{"title":"On computing relative effective population size estimates and parameters from an equilibrium cycle of hermaphrodite frequency fluctuation due to mixed reproductive modes in filamentous fungi","authors":"C. Toomajian","doi":"10.4148/1941-4765.2176","DOIUrl":"https://doi.org/10.4148/1941-4765.2176","url":null,"abstract":"Many filamentous ascomycete fungi reproduce primarily asexually, with only occasional sexual generations. This can lead to a departure from the 1:1 mating type ratio that is expected in obligate sexual populations. The relaxed selection on sexual traits also can lead to a decrease in the frequency of female fertile strains in field populations, while male fertility does not similarly decrease since male gametes also can serve as asexual spores. Both changes ultimately impact the strength of genetic drift in populations. The frequency of female sterility likely increases with the time since the last generation of sexual reproduction, such that it can be used to estimate the relative frequency of sexual reproduction. Here I provide additional details relevant to Leslie and Klein’s (1996) model of mixed sexual reproduction and vegetative propagation as related to the frequency of female sterility. This includes new or modified equations that allow for simpler calculations of i) two estimates of relative Ne, ii) the expected range of hermaphrodite frequencies during the cycles of mixed reproduction, and iii) the relative frequency of sex. These equations also are included in spreadsheet templates into which researchers can directly enter frequencies computed from their population data to estimate these parameters for their own populations. These resources will make the results of Leslie and Klein (1996) more accessible and should increase the use of this model in evaluating the frequency of sexual reproduction of filamentous fungi.","PeriodicalId":12490,"journal":{"name":"Fungal Genetics Reports","volume":"13 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81899501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The split-marker (SM) protocol has become a popular method for production of knockout mutations in fungi. We used Southern hybridization to compare the performance and efficiency of the SM protocol with the more traditional double-crossover intact marker (IM) method for creating deletions of the mating type genes in Fusarium graminearum. Both methods successfully produced knockouts at a rate of between 24 and 75%: the SM method produced mutants more efficiently for larger constructs (>1 kb), but it was similar to IM for a smaller construct that was 865 bp. Both methods also produced strains with additional ectopic integrations at a similar rate of approximately 10%, but on average the SM produced a higher number of independent integrations in those strains. Ectopic integrations produce off-site mutations, and strains with multiple integrations are less desirable since it is more difficult to remove them by backcrossing. Southern hybridizations will be generally superior to PCR to identify strains with fewer ectopic integrations for experimental purposes.
{"title":"Relative efficiency of split-marker versus double-crossover replacement protocols for production of deletion mutants in strain PH-1 of Fusarium graminearum","authors":"S. Bec, Gabdiel Yulfo-Soto, L. Vaillancourt","doi":"10.4148/1941-4765.2175","DOIUrl":"https://doi.org/10.4148/1941-4765.2175","url":null,"abstract":"The split-marker (SM) protocol has become a popular method for production of knockout mutations in fungi. We used Southern hybridization to compare the performance and efficiency of the SM protocol with the more traditional double-crossover intact marker (IM) method for creating deletions of the mating type genes in Fusarium graminearum. Both methods successfully produced knockouts at a rate of between 24 and 75%: the SM method produced mutants more efficiently for larger constructs (>1 kb), but it was similar to IM for a smaller construct that was 865 bp. Both methods also produced strains with additional ectopic integrations at a similar rate of approximately 10%, but on average the SM produced a higher number of independent integrations in those strains. Ectopic integrations produce off-site mutations, and strains with multiple integrations are less desirable since it is more difficult to remove them by backcrossing. Southern hybridizations will be generally superior to PCR to identify strains with fewer ectopic integrations for experimental purposes.","PeriodicalId":12490,"journal":{"name":"Fungal Genetics Reports","volume":"146 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79661124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-11-25DOI: 10.1201/9781003067436-14
M. Farman, S. Leong
{"title":"Genetic Analysis and Mapping of Avirulence Genes in Magnaporthe grisea","authors":"M. Farman, S. Leong","doi":"10.1201/9781003067436-14","DOIUrl":"https://doi.org/10.1201/9781003067436-14","url":null,"abstract":"","PeriodicalId":12490,"journal":{"name":"Fungal Genetics Reports","volume":"46 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79252444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Biology of Fungi","authors":"C. J. Bos","doi":"10.1201/9781003067436-1","DOIUrl":"https://doi.org/10.1201/9781003067436-1","url":null,"abstract":"","PeriodicalId":12490,"journal":{"name":"Fungal Genetics Reports","volume":"19 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80080692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
PROFESSIONAL EDUCATION • Board Certification: Maternal and Fetal Medicine, American Board of Obstetrics and Gynecology (2021) • Board Certification: Obstetrics and Gynecology, American Board of Obstetrics and Gynecology (2018) • Fellowship: UC Irvine Medical Genetics Fellowship (2018) CA • Fellowship: UC Irvine Maternal Fetal Medicine Fellowship (2018) CA • Residency: UC Irvine Obstetrics and Gynecology Residency (2014) CA • Medical Education: University of California at San Francisco School of Medicine (2010) CA
{"title":"Mutation","authors":"C. J. Bos, D. Stadler","doi":"10.1201/9781003067436-2","DOIUrl":"https://doi.org/10.1201/9781003067436-2","url":null,"abstract":"PROFESSIONAL EDUCATION • Board Certification: Maternal and Fetal Medicine, American Board of Obstetrics and Gynecology (2021) • Board Certification: Obstetrics and Gynecology, American Board of Obstetrics and Gynecology (2018) • Fellowship: UC Irvine Medical Genetics Fellowship (2018) CA • Fellowship: UC Irvine Maternal Fetal Medicine Fellowship (2018) CA • Residency: UC Irvine Obstetrics and Gynecology Residency (2014) CA • Medical Education: University of California at San Francisco School of Medicine (2010) CA","PeriodicalId":12490,"journal":{"name":"Fungal Genetics Reports","volume":"2 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81781855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Excellence by Simplicity: Life and Contributions of Professor Ramesh Maheshwari (1940-2019)","authors":"K. Adhvaryu","doi":"10.4148/1941-4765.2171","DOIUrl":"https://doi.org/10.4148/1941-4765.2171","url":null,"abstract":"","PeriodicalId":12490,"journal":{"name":"Fungal Genetics Reports","volume":"26 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78876355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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