Development of antifungal paper from Trametes versicolor containing orange oil against fungal skin infections: A model based on in vitro studies

Sirimas Sukmas, T. Aewsiri, N. Matan
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Abstract

Introduction: Mould infection on the human skin is one of important effects of mould on human health. Skin mould infection is normally caused by Aspegillus, Penicillium and Rhizopus. To date, orange oil has been applied in the present study to inhibit mould infection by adding it into fungal paper. Fungal paper made from Trametes versicolor was used to absorb and slow down the release of orange oil vapour. Therefore, the objective of this work was to investigate the effect of orange oil in fungal paper on growth of infected skin mould as a model in vitro test. Methods: Orange oil and the main component (limonene) was prepared in the Malt Extract Agar (MEA) and added into fungal paper made from T. versicolor at concentrations of 0 (control), 20, 40, 60, 80 and 100 μg g−1. Each of mycelium and spores of Aspergillus niger, Aspergillus flavus, Penicillium chrysogenum and Rhizopus spp were placed on the MEA. A spore of mould was sprayed on the fungal paper. Results: Using 60 μg g−1 of orange oil in MEA and 80 μg g−1 of orange oil in the fungal paper exhibited complete inhibition of growth of all moulds in this test. Limonene alone could not inhibit all moulds in this test; therefore, some minor components of orange can play a significant role in mould growth inhibition. Moreover, fungal paper made from T. versicolor exhibited slow release of orange oil components with active to inhibit mould at least 30 days. Conclusions: Thus, this research demonstrated the potential of fungal paper containing orange oil to control the growth of infected moulds and that this combination could be applied in medicine to control infection of skin in the future. *Correspondence to: Narumol Matan, Food Science and Technology, School of Agricultural Technology, Walailak University, Nakhon Si Thammarat 80160, Thailand and Research Center of Excellence in Innovation of Essential oil, Walailak University, Nakhon Si Thammarat 80160, Thailand, Tel: 6675672359; Fax: 6675672971; E-mail: nnarumol@wu.ac.th; nnarumol@yahoo.com
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含橙油的彩板草抗真菌纸的研制:基于体外研究的模型
人体皮肤霉菌感染是霉菌对人体健康的重要影响之一。皮肤霉菌感染通常是由曲霉、青霉和根霉引起的。迄今为止,本研究已将橙油添加到真菌纸中,以抑制霉菌感染。用彩板菌制成的真菌纸来吸收和减缓橙油蒸汽的释放。因此,本研究以真菌纸中的橙油为模型,探讨其对感染皮肤霉菌生长的影响。方法:在麦芽提取物琼脂(MEA)中制备橙油和主要成分柠檬烯,分别以0(对照)、20、40、60、80和100 μg−1的浓度加入到由花枝霉制成的真菌纸中。将黑曲霉、黄曲霉、黄化青霉和根霉的菌丝体和孢子分别置于MEA上。将霉菌孢子喷在真菌纸上。结果:在MEA中添加60 μg−1的橙油,在真菌纸中添加80 μg−1的橙油,对本试验中所有霉菌的生长均有完全的抑制作用。单用柠檬烯不能抑制所有霉菌;因此,橙中的一些微量成分可以起到显著的抑制霉菌生长的作用。此外,以紫皮霉为原料制成的真菌纸,其橙油成分释放缓慢,对霉菌的抑制作用至少为30天。结论:本研究证明了含橙油真菌纸具有控制感染霉菌生长的潜力,该组合可在未来用于医学上控制皮肤感染。*通讯:Narumol Matan,食品科学与技术,Walailak大学农业技术学院,Nakhon Si Thammarat 80160,泰国;Narumol Matan, Walailak大学精油创新卓越研究中心,Nakhon Si Thammarat 80160,泰国,电话:6675672359;传真:6675672971;电子邮件:nnarumol@wu.ac.th;nnarumol@yahoo.com
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