Study on the Apoptosis Effect Induced by Isothiocyanates in Broccoli on Human Gastric Adenoma Cells (SGC-7901)

2008 2nd International Conference on Bioinformatics and Biomedical Engineering Pub Date : 2008-12-18 DOI:10.1109/FBIE.2008.50

Z. Xiang, Ji Yu-bin, Ji Chen-feng, Yu Lei, L. Lang

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Abstract

To investigate the pro-apoptosis effect of isothiocyanates(ITCS) on human gastric adenoma cells SGC- 7901 and its mechanism. METHODS: SGC-7901 was treated with different concentrations of ITCS. MTT assay was used to evaluate the influence of ITCS on cell proliferation. Flow cytometry was used to test reactive oxygen species (ROS) levels, intracellular mitochondrial transmembrane potential (Deltapsim), and hypodiploid apoptosis peak in both SGC-7901. RESULTS: ITCS obviously inhibited proliferation of SGC-7901 cells. When treated with 0, 15, 30, 60, 120, 240 mug /mL of ITCS for 24 h, intracellular ROS levels of SGC-7901 were (1.6 plusmn 0.5)%, (2.0 plusmn 0.3)%, (5.5 plusmn0.4)%, (25.8 plusmn1.4)%, (83.7 plusmn 1.2)% and (97.4 plusmn 4.2)%, respectively; and Deltapsim were (98.6 plusmn4.3)%, (98.4 plusmn 4.8)%, (95.7 plusmn 5.4)%, (92.6 plusmn 4.0)%, (74.0 plusmn 5.6)% and (63.7 plusmn4.0)%, respectively; when treated with 0, 60, 120, 240 mug /mL of ITCS for 48 h, cell apoptotic rates of SGC-7901 were (4.3 plusmn1.6)%, (9.1 plusmn 3.8)%, (20.1 plusmn 4.2)% and (55.4 plusmn 4.9)%, respectively. CONCLUSIONS: ITCS generates ROS in both SGC-7901, which causes mitochondrial membrane permeabilization and Deltapsim decrease, therefore, leads to apoptosis.

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西兰花异硫氰酸酯诱导人胃腺瘤细胞(SGC-7901)凋亡的研究

探讨异硫氰酸酯(ITCS)对人胃腺瘤细胞SGC- 7901的促凋亡作用及其机制。方法:用不同浓度的ITCS处理SGC-7901。采用MTT法评价ITCS对细胞增殖的影响。流式细胞术检测SGC-7901细胞的活性氧(ROS)水平、细胞内线粒体跨膜电位(Deltapsim)和次二倍体凋亡峰值。结果:ITCS对SGC-7901细胞增殖有明显抑制作用。在0、15、30、60、120、240杯/mL的ITCS作用24 h时，SGC-7901细胞内ROS水平分别为(1.6 plusmn 0.5)%、(2.0 plusmn 0.3)%、(5.5 plusmn0.4)%、(25.8 plusmn1.4)%、(83.7 plusmn 1.2)%和(97.4 plusmn 4.2)%;和Deltapsim分别为(98.6 plusmn4.3)%、(98.4 plusmn4.8)%、(95.7 plusmn5.4)%、(92.6 plusmn4.0)%、(74.0 plusmn5.6)%和(63.7 plusmn4.0)%;0、60、120、240 mg /mL的ITCS作用48 h, SGC-7901细胞凋亡率分别为(4.3 plusmn1.6)%、(9.1 plusmn 3.8)%、(20.1 plusmn 4.2)%和(55.4 plusmn 4.9)%。结论:ITCS在SGC-7901中均产生ROS，导致线粒体膜通透性和Deltapsim降低，从而导致细胞凋亡。

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2008 2nd International Conference on Bioinformatics and Biomedical Engineering

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