Study on the Apoptosis Effect Induced by Isothiocyanates in Broccoli on Human Gastric Adenoma Cells (SGC-7901)

Abstract

To investigate the pro-apoptosis effect of isothiocyanates(ITCS) on human gastric adenoma cells SGC- 7901 and its mechanism. METHODS: SGC-7901 was treated with different concentrations of ITCS. MTT assay was used to evaluate the influence of ITCS on cell proliferation. Flow cytometry was used to test reactive oxygen species (ROS) levels, intracellular mitochondrial transmembrane potential (Deltapsim), and hypodiploid apoptosis peak in both SGC-7901. RESULTS: ITCS obviously inhibited proliferation of SGC-7901 cells. When treated with 0, 15, 30, 60, 120, 240 mug /mL of ITCS for 24 h, intracellular ROS levels of SGC-7901 were (1.6 plusmn 0.5)%, (2.0 plusmn 0.3)%, (5.5 plusmn0.4)%, (25.8 plusmn1.4)%, (83.7 plusmn 1.2)% and (97.4 plusmn 4.2)%, respectively; and Deltapsim were (98.6 plusmn4.3)%, (98.4 plusmn 4.8)%, (95.7 plusmn 5.4)%, (92.6 plusmn 4.0)%, (74.0 plusmn 5.6)% and (63.7 plusmn4.0)%, respectively; when treated with 0, 60, 120, 240 mug /mL of ITCS for 48 h, cell apoptotic rates of SGC-7901 were (4.3 plusmn1.6)%, (9.1 plusmn 3.8)%, (20.1 plusmn 4.2)% and (55.4 plusmn 4.9)%, respectively. CONCLUSIONS: ITCS generates ROS in both SGC-7901, which causes mitochondrial membrane permeabilization and Deltapsim decrease, therefore, leads to apoptosis.