Cell, protoplast and tissue culture of short-rotation species

Abstract

The undertakings of this activity are described, including workshops, tree form survey, and, in depth, an example of research undertaken: gamma-irradiated and non-irradiated cell suspensions of hybrid poplar NM1 (Populus nigra × P. maximowiczii) were used to select cells that had a higher tolerance to the active ingredient in the herbicide Roundup. Numerous cell lines were recovered that were able to grow, at about 60% of the control lines, in 10 mm glyphosate. These cells were transferred to an embedded single-cell regeneration system which was used to recover calli regenerated in the presence of 10 mM glyphosate. These were transferred to glyphosate-free medium for shoot elongation.