Determination of the Active Chemical Compounds and the Antibacterial Activity of Various Fractions of Lawsonia inermis L.

S. Mulyaningsih, Febriyati Adji Rachmadani
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Abstract

Lawsonia inermis L., or henna leaves, are usually used to treat wounds on the skin. Lawsonia inermis contain naphthoquinones, flavonoids, tannins, and saponins. The antibacterial activity of L. inermis leaf extract in various solvents has been extensively studied. However, which component is responsible for the antibacterial activity is still unknown. This study was intended to investigate the antibacterial effect of L. inermis fractions against Staphylococcus aureus and to discover the antibacterial chemical class in the most active fraction. The methanol extract was fractionated with n-hexane and ethyl acetate subsequently. The antibacterial activity of various fractions was tested using the well diffusion method. TLC-bioautography was used to identify the class of active chemicals as antibacterial agents. Antibacterial activity against S. aureus was highest in the ethyl acetate fraction. TLC-bioautography of the ethyl acetate fraction showed inhibition areas at Rf values of 0.25 and 0.53, respectively, indicating the naphthoquinones and phenolic compounds groups. In conclusion, naphthoquinones and phenolic compounds are suggested to contribute to the antibacterial effect of the ethyl acetate fraction of L. inermis leaves.
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刺槐不同部位活性成分及抑菌活性的测定。
指甲花通常用于治疗皮肤上的伤口。月桂草含有萘醌类、类黄酮、单宁和皂苷。人们对羊草叶提取物在各种溶剂中的抑菌活性进行了广泛的研究。然而,哪一种成分是抗菌活性的原因尚不清楚。摘要本研究旨在研究牛乳草提取物对金黄色葡萄球菌的抑菌作用,并找出其活性部位的抑菌化学类。甲醇提取物经正己烷和乙酸乙酯分馏。采用孔扩散法测定各组分的抑菌活性。采用薄层-生物自显影法鉴别抗菌活性物质的种类。乙酸乙酯部位对金黄色葡萄球菌的抑菌活性最高。乙酸乙酯部分的tlc生物图谱显示,抑制区分别为0.25和0.53,表明其为萘醌类和酚类化合物基团。综上所述,槲寄生叶乙酸乙酯部位的抑菌作用可能与萘醌类和酚类化合物有关。
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