Potential impact of Paracentrotus lividus extract on diabetic rat models induced by high fat diet/streptozotocin

Abstract

Antioxidant therapy has been thought to be effectual for the prevention and treatment of various diseases including diabetes. Therefore, the present study was designed to investigate the potency of Paracentrotus lividus extract (PLE) for alleviating the complications that resulted after induction of the diabetic rat models (T1DM and T2DM) using high fat diet (HFD)/streptozotocin (STZ). Thirty six male Wistar albino rats were assigned into normal control, T1DM and T2DM untreated, and PLE treated diabetic rat groups. Induction of T1DM was performed by streptozotocin injection (60 mg/kg of dissolved in sodium citrate buffer, 0.1 mol/L, i.p). T2DM induction through 4 weeks of high fat diet (HFD) intervention was followed by a single low dosage of STZ (30 mg/kg dissolved in 0.1 mol/L citrate buffer at pH 4.5, i.p). Both diabetic rat models showed a significant increase in serum; levels of fasting glucose, total protein, bilirubin, activities of arginase, transaminases (AST and ALT), alkaline phosphatase (ALP), γ glutamyl transferase (GGT), lipid profile parameters, and liver malondialdehyde (MDA). However, T1DM and T2DM rats have decreased levels of serum insulin, and liver glucose 6 phosphate dehydrogenase (G6PD), glutathione reduced (GSH), nitric oxide (NO), and antioxidant enzymes. Furthermore, the present study showed the hypoglycemic, hypolipidemic, and antioxidant potency of the PLE as confirmed by its ability for ameliorating most of the alterations caused in the studied parameters of diabetic rats. In conclusion, PLE may be useful as therapy against oxidative stress and liver damage in both types of diabetes mellitus and is therefore recommended for further studies.