Effect of Neem Extract with Zinc Oxide Nanoparticles for Burn Wound Treatment

Abstract

A study was conducted to determine the clinical efficiency of neem extract with Zinc Oxide (ZnO) nanoparticles on burn wound healing treatment in Rabbit model. Sixteen (16) male New Zealand white rabbits were assigned into four groups, each with four rabbits, and were kept together for 28 days. Group T0 as the control; T1 represented burn healing using neem extract; T2 represented burn healing using neem extract with ZnO nanoparticles; and T3 represented burn healing using a commercial drug (Bactrocin®). Data were accumulated on the healing rate, healing time, and WBC count. Healing rate was calculated at weekly interval and the healing time was observed regularly and calculated from day 7 at weekly interval and WBC count were investigated using an auto analyzer. The result showed that the healing rate (%) were T2 group= 93.75±1.25, T3 group=86.75±0.479, T1 group=84.25±1.75, and T0 group=70.75±2.25. Among this result the healing rate increased significantly in T2 (93.75±1.25) group, better by T3 (86.75±0.479), good by T1 (84.25±1.75) and worst by T0 (70.75±2.25) group. The application of neem extract with ZnO nanoparticles have also showed the quickest healing time (24.5±0.64 day) significantly (P<0.01). At weekly interval WBC count (cells/µl) was varied significantly, the highest value was discovered in the T2 group. The study's overall findings demonstrated that using neem extract with ZnO nanoparticles resulted in faster wound healing with a shorter time requirement. It indicates that wound healing without the use of antibiotics was possible and using neem extract can be advised for the treatment of burn wounds.