PURELY CLEAR KINETIC SPECTROPHOTOMETRIC PROCESS FOR BROMHEXINE HYDROCHLORIDE EVALUATION IN PHARMACEUTICAL PREPARATIONS

Abstract

For the evaluation of Bromhexine hydrochloride (BRO) in pure drugs as well as their pharmaceutical preparations, a successful, sensitivity spectrophotometric method has been proved as well as checked. The methodology is focused on the creation of the Schiff base by pdimethylaminobenzaldehyde (PDMB), giving a bright yellow color to the reaction of the drug with the reagent . The yellow colored absorbance of species was assessed at its maximum absorption λmax of 420 nm. The Beer’s law was obeyed in the 10-60 μg/ml as the concentration range. The optical values were measured to be 8.693 x10 (L/mol/cm), 0.0005 (μg/cm2), Molar absorption coefficient as well as Sandell sensitivity respectively. The LOD and the LOQ for the suggested methodology were measured 3.280μg/ml, 4.114 μg/ml, respectively. To maximize the reaction conditions, all the variables were studied. The interference for the suggested method was identified in the existence of traditional excipients of pharmaceutics. By testing BRO in its pharmaceutical formulations, the validity of the approach was checked and objectively tested by statistical tests for its accuracy. Strong recoveries were obtained by the method developed; the results obtained in its pharmaceutical dosage forms were critically evaluated and used effectively for determining BRO.