Pub Date : 2023-01-01DOI: 10.47743/achi-2023-1-0005
A. Sani, Aisha Kabir
The powdered roots and stem bark of Jatropha curcas were extracted using 95% ethanol. The ethanol extracts obtained from parts of plants were partitioned into fractions using chloroform, distilled water, ethyl acetate, methanol and n-hexane solvents of varying polarity. The crude ethanol extract and five soluble fractions of both the roots and stem bark were tested against plasmodium falciparum. The results shown a good anti-plasmodial potency at high concentration of the fraction of the roots and stem bark of J. curcas. Chloroform and n-hexane soluble fractions recorded good activity on the test organisms with 94.3% and 97.1% inhibition. The results indicated that the roots and stem bark extracts contained active compounds which have anti-malaria property. Test for the presence of secondary metabolites in the plant extracts revealed the presence of saponins, flavonoids, steroids, tannins and alkaloids.
{"title":"ANTIPLASMODIAL ACTIVITY AND PHYTOCHEMICAL ANALYSIS OF EXTRACTS FROM THE ROOTS AND STEM BARK OF JATROPHA CURCAS","authors":"A. Sani, Aisha Kabir","doi":"10.47743/achi-2023-1-0005","DOIUrl":"https://doi.org/10.47743/achi-2023-1-0005","url":null,"abstract":"The powdered roots and stem bark of Jatropha curcas were extracted using 95% ethanol. The ethanol extracts obtained from parts of plants were partitioned into fractions using chloroform, distilled water, ethyl acetate, methanol and n-hexane solvents of varying polarity. The crude ethanol extract and five soluble fractions of both the roots and stem bark were tested against plasmodium falciparum. The results shown a good anti-plasmodial potency at high concentration of the fraction of the roots and stem bark of J. curcas. Chloroform and n-hexane soluble fractions recorded good activity on the test organisms with 94.3% and 97.1% inhibition. The results indicated that the roots and stem bark extracts contained active compounds which have anti-malaria property. Test for the presence of secondary metabolites in the plant extracts revealed the presence of saponins, flavonoids, steroids, tannins and alkaloids.","PeriodicalId":6958,"journal":{"name":"Acta Chemica Iasi","volume":"19 1","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75454757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.47743/achi-2023-1-0001
Maha Nasr, M. Al-Rufaie
The goal of this study is to develop a simple and precise spectrophotometric method for the quantitative determination of chlorpromazine hydrochloride (CPZ.HCL) by its reaction with platinum tetrachloride (PtC) in aqueous solutions (acidic medium) using UV-Vis spectrophotometry. Accordingly, a blue complex was formed having a characteristic band at 533 nm. To obtain high sensitivity and good stability, optimal reaction conditions were investigated, like the influence of pH, temperature, time, and the optimal concentration of platinum tetrachloride, as well as calibration curves were generated. Results indicated the possibility of using this method in estimating the drug above, as the calibration curve was subject to Beer's law for the range 5-75 µg/mL, and the molar absorptivity, Sandell's sensitivity, detection limit, and quantitative limit were 1563.32 L/mole·cm, 0.2272 µg/cm2 , 0.5102 and 1.7007 µg/mL respectively. The proposed procedure has also been successfully applied for CPZ·HCL quantification in the pharmaceutical form.
{"title":"UV–VISIBLE SPECTROPHOTOMETRIC DETERMINATION OF CHLORPROMAZINE HYDROCHLORIDE FROM PHARMACEUTICALS USING PLATINUM (IV) CHLORIDE","authors":"Maha Nasr, M. Al-Rufaie","doi":"10.47743/achi-2023-1-0001","DOIUrl":"https://doi.org/10.47743/achi-2023-1-0001","url":null,"abstract":"The goal of this study is to develop a simple and precise spectrophotometric method for the quantitative determination of chlorpromazine hydrochloride (CPZ.HCL) by its reaction with platinum tetrachloride (PtC) in aqueous solutions (acidic medium) using UV-Vis spectrophotometry. Accordingly, a blue complex was formed having a characteristic band at 533 nm. To obtain high sensitivity and good stability, optimal reaction conditions were investigated, like the influence of pH, temperature, time, and the optimal concentration of platinum tetrachloride, as well as calibration curves were generated. Results indicated the possibility of using this method in estimating the drug above, as the calibration curve was subject to Beer's law for the range 5-75 µg/mL, and the molar absorptivity, Sandell's sensitivity, detection limit, and quantitative limit were 1563.32 L/mole·cm, 0.2272 µg/cm2 , 0.5102 and 1.7007 µg/mL respectively. The proposed procedure has also been successfully applied for CPZ·HCL quantification in the pharmaceutical form.","PeriodicalId":6958,"journal":{"name":"Acta Chemica Iasi","volume":"36 1","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81385382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.47743/achi-2023-1-0004
T. Jassim, Raisan Kadhim Taresh, A. Jawad, M. Al-Rufaie
In this manuscript, to determine resorcinol (RES) in pure and pharmaceutical forms, we developed a simple colorimetric method based on the formation of environmentally friendly silver nanoparticles (oxidizing agent). The electron cloud formed silver nanoparticles (AgNPs) in an alkaline medium and then reacted (RES) in the presence of Polyvinylpyrrolidone as a stabilizer in nanometer dimensions. It can oscillate on particle surfaces when dispersed in a liquid medium. It showed adequate absorption at a wavelength of 416 nm. Lambert-Beer's law is linear from 1.6 - 12 ppm, and the analysis method was simple, sensitive and highly selective.
{"title":"SPECTROPHOTOMETRIC COLORIMETRIC DETERMINATION FOR RESORCINOL DURING SILVER NANOPARTICLES FORMATION WITH A FOCUSED PLASMON RESONANCE SURFACE USING POLYVINYL PYRROLIDONE AS STABILIZER","authors":"T. Jassim, Raisan Kadhim Taresh, A. Jawad, M. Al-Rufaie","doi":"10.47743/achi-2023-1-0004","DOIUrl":"https://doi.org/10.47743/achi-2023-1-0004","url":null,"abstract":"In this manuscript, to determine resorcinol (RES) in pure and pharmaceutical forms, we developed a simple colorimetric method based on the formation of environmentally friendly silver nanoparticles (oxidizing agent). The electron cloud formed silver nanoparticles (AgNPs) in an alkaline medium and then reacted (RES) in the presence of Polyvinylpyrrolidone as a stabilizer in nanometer dimensions. It can oscillate on particle surfaces when dispersed in a liquid medium. It showed adequate absorption at a wavelength of 416 nm. Lambert-Beer's law is linear from 1.6 - 12 ppm, and the analysis method was simple, sensitive and highly selective.","PeriodicalId":6958,"journal":{"name":"Acta Chemica Iasi","volume":"41 1","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79393733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.47743/achi-2023-1-0006
Baneen Sami Tarif, Zainab Hussein AL-Hellawi
A metabolic condition known as type 2 diabetes mellitus (T2DM) has spread all over the world. Pancreatic insulin resistance and β-cell dysfunction are its defining features. In 2021, almost 537 million people worldwide had diabetes. The prevalence of hypertension (HTN) in T2DM more than 50% result from uncontrolled blood glucose. The prevalence of microvascular and macrovascular complications in T2DM with HTN, more common than in those without HTN. Hyperglycemia is related to overproduction of reactive oxygen species (ROS) and oxidative stress (OS). The etiology of metabolic disorders is associated with OS. On the other hand, OS increases the risk of cardiovascular disease (CVD) by causing endothelial dysfunction. An important source of ROS is xanthine oxidase (XOD), while uric acid (UA) has two properties: pro- oxidant and antioxidant. Sixty T2DM patients with and without hypertension participated in this research. These patients were compared to a control group of thirty people. Lipid profile, XOD and UA were determined in this research. The results of the study showed a significant increase in XOD and UA values in T2DM patients compared to controls. The elevated OS agents and lipids biomarkers mainly in T2DM group could contribute to increasing cardiometabolic risks and weakening the overall health status of the participants. Triglyceride (TG), total cholesterol (TC), LDL-c and HDL-c abnormalities are risk factors of CVD. Dyslipidemia and elevated HbA1c in diabetic patients can be considered as a very high-risk group for CVD.
{"title":"TYPE 2 DIABETES MULLITUS PATIENTS WITH AND WITHOUT HYPERTENSION: XANTHINE OXIDASE ACTIVITY AND URIC ACID CONCENTRATION","authors":"Baneen Sami Tarif, Zainab Hussein AL-Hellawi","doi":"10.47743/achi-2023-1-0006","DOIUrl":"https://doi.org/10.47743/achi-2023-1-0006","url":null,"abstract":"A metabolic condition known as type 2 diabetes mellitus (T2DM) has spread all over the world. Pancreatic insulin resistance and β-cell dysfunction are its defining features. In 2021, almost 537 million people worldwide had diabetes. The prevalence of hypertension (HTN) in T2DM more than 50% result from uncontrolled blood glucose. The prevalence of microvascular and macrovascular complications in T2DM with HTN, more common than in those without HTN. Hyperglycemia is related to overproduction of reactive oxygen species (ROS) and oxidative stress (OS). The etiology of metabolic disorders is associated with OS. On the other hand, OS increases the risk of cardiovascular disease (CVD) by causing endothelial dysfunction. An important source of ROS is xanthine oxidase (XOD), while uric acid (UA) has two properties: pro- oxidant and antioxidant. Sixty T2DM patients with and without hypertension participated in this research. These patients were compared to a control group of thirty people. Lipid profile, XOD and UA were determined in this research. The results of the study showed a significant increase in XOD and UA values in T2DM patients compared to controls. The elevated OS agents and lipids biomarkers mainly in T2DM group could contribute to increasing cardiometabolic risks and weakening the overall health status of the participants. Triglyceride (TG), total cholesterol (TC), LDL-c and HDL-c abnormalities are risk factors of CVD. Dyslipidemia and elevated HbA1c in diabetic patients can be considered as a very high-risk group for CVD.","PeriodicalId":6958,"journal":{"name":"Acta Chemica Iasi","volume":"20 1","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78654282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.47743/achi-2023-1-0002
A. M. Saad, Z. Motaweq, A. Al-Marzoqi
: One of the most prevalent prostatic diseases among young and middle-aged men is prostatitis. The prostate gland infections constitute a significant problem that affects men at all ages. Due to shortcomings in the diagnostic procedures, the prevalence of this type of prostate gland inflammation is still unknown. The goal of this investigation is to quantify the level of two anti-inflammatory and pro-inflammatory cytokines such as Interleukin 10 and Interleukin 1α, respectively in the framework of prostatic gland infections sickness. The result showed an increase of cytokine concentration (level) such as IL-10 and IL-1α in the serum of prostatitis patients. Average serum IL-10 and IL-1 α levels in serum of patients was 332.01± 9.07 and 54.55±7.69, respectively compared with control was 205.98±17.41 and 55.25±13.11. IL-10 and IL-1α concentrations in patient sera were significantly higher than those in control sera, respectively, and this demonstrated their significance .
{"title":"STUDY ROLE OF INTERLEUKIN-10 AND INTERLEUKIN-1Α ON PATIENTS WITH PROSTATITIS IN BABYLON PROVINCE, IRAQ","authors":"A. M. Saad, Z. Motaweq, A. Al-Marzoqi","doi":"10.47743/achi-2023-1-0002","DOIUrl":"https://doi.org/10.47743/achi-2023-1-0002","url":null,"abstract":": One of the most prevalent prostatic diseases among young and middle-aged men is prostatitis. The prostate gland infections constitute a significant problem that affects men at all ages. Due to shortcomings in the diagnostic procedures, the prevalence of this type of prostate gland inflammation is still unknown. The goal of this investigation is to quantify the level of two anti-inflammatory and pro-inflammatory cytokines such as Interleukin 10 and Interleukin 1α, respectively in the framework of prostatic gland infections sickness. The result showed an increase of cytokine concentration (level) such as IL-10 and IL-1α in the serum of prostatitis patients. Average serum IL-10 and IL-1 α levels in serum of patients was 332.01± 9.07 and 54.55±7.69, respectively compared with control was 205.98±17.41 and 55.25±13.11. IL-10 and IL-1α concentrations in patient sera were significantly higher than those in control sera, respectively, and this demonstrated their significance .","PeriodicalId":6958,"journal":{"name":"Acta Chemica Iasi","volume":"89 1","pages":""},"PeriodicalIF":0.5,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91296311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.47743/achi-2022-2-0007
Ancuța-Veronica Lupăescu, Laura Ion-Darie, B. Petre, F. Ursachi, M. Oroian
: The identification of compounds with antioxidant activity is considered to be of great interest to both the pharmaceutical and cosmetic industries. Bee products such as pollen, propolis, beeswax and royal jelly have long been used for their medicinal and health-enhancing properties. Bee pollen is a raw product rich in secondary metabolites and high concentrations of phytochemicals and nutrients. Protein is contained in the pollen matrix at an average of 22.7% and its content varies among plant species. The present study aimed to analyze the protein content of linden and hawthorn pollen. After a delipidation step, pollen samples were extracted using ultrasonic energy and three different extraction buffers. After quantification by the Bradford colorimetric method, protein extracts were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Fourier transform infrared spectroscopy. Finally, we evaluated the total antioxidant capacity of pollen extracts using the iron(III) reduced antioxidant capacity assay.
{"title":"PROTEOMIC ANALYSIS AND ANTIOXIDANT EVALUATION OF ULTRASOUND - ASSISTED POLLEN EXTRACTS","authors":"Ancuța-Veronica Lupăescu, Laura Ion-Darie, B. Petre, F. Ursachi, M. Oroian","doi":"10.47743/achi-2022-2-0007","DOIUrl":"https://doi.org/10.47743/achi-2022-2-0007","url":null,"abstract":": The identification of compounds with antioxidant activity is considered to be of great interest to both the pharmaceutical and cosmetic industries. Bee products such as pollen, propolis, beeswax and royal jelly have long been used for their medicinal and health-enhancing properties. Bee pollen is a raw product rich in secondary metabolites and high concentrations of phytochemicals and nutrients. Protein is contained in the pollen matrix at an average of 22.7% and its content varies among plant species. The present study aimed to analyze the protein content of linden and hawthorn pollen. After a delipidation step, pollen samples were extracted using ultrasonic energy and three different extraction buffers. After quantification by the Bradford colorimetric method, protein extracts were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Fourier transform infrared spectroscopy. Finally, we evaluated the total antioxidant capacity of pollen extracts using the iron(III) reduced antioxidant capacity assay.","PeriodicalId":6958,"journal":{"name":"Acta Chemica Iasi","volume":"36 1","pages":""},"PeriodicalIF":0.5,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73207696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.47743/achi-2022-1-0003
Hussen Abdu, D. Isabel, Adgo Tsegaye Abebaw, Taddesse Mengesha Abi
: The increasing of toxic heavy metal and nitrate ions contamination in water and food systems worldwide has become a core problem. Therefore, the development of real-time, highly sensitive and selective, simple technique for nitrate and toxic heavy metals ions (mercury, copper and arsenic) detection in water and food at ultralow concentrations are important for maintaining their safe deliveries to consumers. A highly efficient fluorescent chemosensor-based on polyaniline supported g-C 3 N 4 /CeO 2 nanocomposite for selective heavy metal and nitrate ions have been successfully developed in this research, by in situ polymerization method. The structural, morphological, and optical properties of the synthesized nanocomposites were characterized by using powder X-ray diffraction (XRD), Fourier- Transform Infrared spectroscopy (FT-IR), Scanning Electron Microscope (SEM), Photoluminescence (PL) and UV-Vis spectroscopy. In the absence of metal and nitrate ions, the nanocomposites exhibit high fluorescence intensity. However, the strong coordination of the basic sites to metal and nitrate ions, causes fluorescence quenching via photoinduced electron transfer and static quenching leading to the qualitative and quantitative detection of metal and nitrate ions. This fluorescent chemosensor exhibits high selectivity toward arsenic (III), copper (II), mercury (II) and nitrate ion. The sensor was more sensitive for copper (II) ion than arsenic (III), mercury (II) and nitrate ions because (II) ion at (3.25x10 4 M -1 ) compared to 8.12 x 10 3 M -1 , 2.93 x 10 4 M -1 and 3.19 x 10 2 M -1 for arsenic (III), mercury (II) and nitrate ions respectively. The practical use of this sensor for arsenic (III), copper (II), mercury (II) and nitrate ions determination in Coca-cola, tap water, milk and lettuce samples respectively, were also applied. The amounts of mercury and nitrate concentrations measured in milk and lettuce were 56.66 μM and 3.18 mM, exceeding the allowable limits stated by WHO (0.1 μM for mercury and 5.9 μM for nitrate, respectively).
在世界范围内，水和食品系统中有毒重金属和硝酸盐离子污染的增加已成为一个核心问题。因此，开发实时、高灵敏度、高选择性、简单的技术，以超低浓度检测水和食物中的硝酸盐和有毒重金属离子(汞、铜和砷)，对于确保其安全交付给消费者至关重要。本研究采用原位聚合的方法，成功制备了一种基于聚苯胺负载的g- c3n4 / ceo2纳米复合材料的高效荧光化学传感器，用于选择性重金属和硝酸盐离子。采用粉末x射线衍射(XRD)、傅里叶变换红外光谱(FT-IR)、扫描电镜(SEM)、光致发光(PL)和紫外可见光谱(UV-Vis)对合成的纳米复合材料的结构、形貌和光学性能进行了表征。在不含金属离子和硝酸盐离子的情况下，纳米复合材料具有较高的荧光强度。然而，碱性位点与金属和硝酸盐离子的强配位，通过光致电子转移和静态猝灭导致荧光猝灭，从而对金属和硝酸盐离子进行定性和定量检测。该荧光化学传感器对砷(III)、铜(II)、汞(II)和硝酸盐离子具有高选择性。该传感器对铜(II)离子比砷(III)、汞(II)和硝酸盐离子更敏感，因为(II)离子对砷(III)、汞(II)和硝酸盐离子的灵敏度分别为(3.25 × 10.4 M -1)、8.12 × 10.3 M -1、2.93 × 10.4 M -1和3.19 × 10.2 M -1，而对铜(II)离子的灵敏度为(3.25 × 10.4 M -1)。该传感器还应用于可口可乐、自来水、牛奶和生菜样品中砷(III)、铜(II)、汞(II)和硝酸盐离子的测定。牛奶和生菜中汞和硝酸盐的浓度分别为56.66 μM和3.18 mM，超过了世界卫生组织规定的允许限值(汞和硝酸盐的浓度分别为0.1 μM和5.9 μM)。
{"title":"POLYANILINE SUPPORTED g-C3N4/CeO2 FLUORESCENT CHEMOSENSOR FOR SELECTED HEAVY METAL AND NITRATE IONS DETERMINATION","authors":"Hussen Abdu, D. Isabel, Adgo Tsegaye Abebaw, Taddesse Mengesha Abi","doi":"10.47743/achi-2022-1-0003","DOIUrl":"https://doi.org/10.47743/achi-2022-1-0003","url":null,"abstract":": The increasing of toxic heavy metal and nitrate ions contamination in water and food systems worldwide has become a core problem. Therefore, the development of real-time, highly sensitive and selective, simple technique for nitrate and toxic heavy metals ions (mercury, copper and arsenic) detection in water and food at ultralow concentrations are important for maintaining their safe deliveries to consumers. A highly efficient fluorescent chemosensor-based on polyaniline supported g-C 3 N 4 /CeO 2 nanocomposite for selective heavy metal and nitrate ions have been successfully developed in this research, by in situ polymerization method. The structural, morphological, and optical properties of the synthesized nanocomposites were characterized by using powder X-ray diffraction (XRD), Fourier- Transform Infrared spectroscopy (FT-IR), Scanning Electron Microscope (SEM), Photoluminescence (PL) and UV-Vis spectroscopy. In the absence of metal and nitrate ions, the nanocomposites exhibit high fluorescence intensity. However, the strong coordination of the basic sites to metal and nitrate ions, causes fluorescence quenching via photoinduced electron transfer and static quenching leading to the qualitative and quantitative detection of metal and nitrate ions. This fluorescent chemosensor exhibits high selectivity toward arsenic (III), copper (II), mercury (II) and nitrate ion. The sensor was more sensitive for copper (II) ion than arsenic (III), mercury (II) and nitrate ions because (II) ion at (3.25x10 4 M -1 ) compared to 8.12 x 10 3 M -1 , 2.93 x 10 4 M -1 and 3.19 x 10 2 M -1 for arsenic (III), mercury (II) and nitrate ions respectively. The practical use of this sensor for arsenic (III), copper (II), mercury (II) and nitrate ions determination in Coca-cola, tap water, milk and lettuce samples respectively, were also applied. The amounts of mercury and nitrate concentrations measured in milk and lettuce were 56.66 μM and 3.18 mM, exceeding the allowable limits stated by WHO (0.1 μM for mercury and 5.9 μM for nitrate, respectively).","PeriodicalId":6958,"journal":{"name":"Acta Chemica Iasi","volume":"156 1","pages":""},"PeriodicalIF":0.5,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73160799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.47743/achi-2022-2-0006
Nasr Maha Salah, Al-Rufaie Mohauman Mohammad
: Trifluoperazine hydrochloride (TFPH) is widely used in the treatment of some diseases such as hysteria, vomiting and schizophrenia. This study aims to develop a fast, easy, sensitive, accurate and economical spectrophotometric method for the determination of TFPH. This method included the reaction of TFPH with Diammonium Cerium Nitrate (CAN) in a medium with a pH value of 1 to form a complex. Parameters influencing the stability of the resulting complex (i.e. time; and temperature) were also investigated. The method depended on the UV spectrum at the wavelength of (499 nm) and the correlation coefficient of (R 2 = 0.9826). The results showed that the method has a good accuracy as (Rec% = 98.925), detection limit (0.408) and quantitative limit (1.361). The linearity range was 2-80 µg/mL. Moreover, this method has been successfully applied for the determination of the drug in its pure form and in its pharmaceutical preparation.
{"title":"SPECTROPHOTOMETRIC DETERMINATION OF TRIFLUOPERAZINE HYDROCHLORIDE USING DIAMMONIUM CERIUM NITRATE IN ITS PURE AND PHARMACEUTICAL FORM","authors":"Nasr Maha Salah, Al-Rufaie Mohauman Mohammad","doi":"10.47743/achi-2022-2-0006","DOIUrl":"https://doi.org/10.47743/achi-2022-2-0006","url":null,"abstract":": Trifluoperazine hydrochloride (TFPH) is widely used in the treatment of some diseases such as hysteria, vomiting and schizophrenia. This study aims to develop a fast, easy, sensitive, accurate and economical spectrophotometric method for the determination of TFPH. This method included the reaction of TFPH with Diammonium Cerium Nitrate (CAN) in a medium with a pH value of 1 to form a complex. Parameters influencing the stability of the resulting complex (i.e. time; and temperature) were also investigated. The method depended on the UV spectrum at the wavelength of (499 nm) and the correlation coefficient of (R 2 = 0.9826). The results showed that the method has a good accuracy as (Rec% = 98.925), detection limit (0.408) and quantitative limit (1.361). The linearity range was 2-80 µg/mL. Moreover, this method has been successfully applied for the determination of the drug in its pure form and in its pharmaceutical preparation.","PeriodicalId":6958,"journal":{"name":"Acta Chemica Iasi","volume":"9 1","pages":""},"PeriodicalIF":0.5,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79616745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.47743/achi-2022-1-0002
Siyum Fikadu, T. Molla, Shewarega Yared, Abraham A. Getahun
: Wheat is one of the major cereal crops of the world ranking second after rice both in area coverage and production among cereal grains. It is also an important cereal crop in Ethiopia in terms of cultivated area, production and consumption, which is the fourth most widely grown crop after teff, maize, and sorghum. It has great importance in the human diet, contributing as a direct source of energy, protein, vitamins, minerals and fiber. However, this cereal crop is vulnerable to degradation by aflatoxins mainly by Aspergillus flavus, Aspergillus Parasiticus and Aspergillus nomius, which have adverse health effects on humans and livestock that ingest aflatoxin contaminated food products and feeds. The contamination of aflatoxins can occur through its growth, harvest, transport and storage. Aflatoxins are carcinogenic substances and are extremely toxic to humans when consuming aflatoxin contaminated foods. Therefore, this study has been undertaken to determine aflatoxin (B1, B2, G1 and G2) in wheat samples collected from two market places (namely, Arada and Azezo) of Gondar town, by using LC-MS/MS. The extraction solvents were acetonitrile and water (84:16 v/v %). Good linearity from the calibration curve was obtained in standard solution of aflatoxins in range between 0.1 and 15 ng/mL, with regression coefficient (R 2 ) values of > 0.999. Limit of detection of the aflatoxins were 0.1208, 0.0302, 0.0328 and 0.1272 µg/kg for aflatoxin B1, B2, G1 and G2, respectively. The average percentage recoveries of spiked samples were range between 70.80 and 77.23 %. Aflatoxins were not detected in both wheat samples from Arada and Azezo. 15 pre-harvest 15 post-harvest
{"title":"DETERMINATION OF AFLATOXIN IN COMMERCIALLY AVAILABLE WHEAT SAMPLES FROM MARKETS OF GONDAR TOWN USING LC-MS/MS","authors":"Siyum Fikadu, T. Molla, Shewarega Yared, Abraham A. Getahun","doi":"10.47743/achi-2022-1-0002","DOIUrl":"https://doi.org/10.47743/achi-2022-1-0002","url":null,"abstract":": Wheat is one of the major cereal crops of the world ranking second after rice both in area coverage and production among cereal grains. It is also an important cereal crop in Ethiopia in terms of cultivated area, production and consumption, which is the fourth most widely grown crop after teff, maize, and sorghum. It has great importance in the human diet, contributing as a direct source of energy, protein, vitamins, minerals and fiber. However, this cereal crop is vulnerable to degradation by aflatoxins mainly by Aspergillus flavus, Aspergillus Parasiticus and Aspergillus nomius, which have adverse health effects on humans and livestock that ingest aflatoxin contaminated food products and feeds. The contamination of aflatoxins can occur through its growth, harvest, transport and storage. Aflatoxins are carcinogenic substances and are extremely toxic to humans when consuming aflatoxin contaminated foods. Therefore, this study has been undertaken to determine aflatoxin (B1, B2, G1 and G2) in wheat samples collected from two market places (namely, Arada and Azezo) of Gondar town, by using LC-MS/MS. The extraction solvents were acetonitrile and water (84:16 v/v %). Good linearity from the calibration curve was obtained in standard solution of aflatoxins in range between 0.1 and 15 ng/mL, with regression coefficient (R 2 ) values of > 0.999. Limit of detection of the aflatoxins were 0.1208, 0.0302, 0.0328 and 0.1272 µg/kg for aflatoxin B1, B2, G1 and G2, respectively. The average percentage recoveries of spiked samples were range between 70.80 and 77.23 %. Aflatoxins were not detected in both wheat samples from Arada and Azezo. 15 pre-harvest 15 post-harvest","PeriodicalId":6958,"journal":{"name":"Acta Chemica Iasi","volume":"18 1","pages":""},"PeriodicalIF":0.5,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86787036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.47743/achi-2022-2-0005
Eswadi Aya Refat, AL-Hellawi Zainab Hussein
: Type 2 Diabetes Mellitus (T2DM) is a common chronic metabolic disease that spread worldwide. Uncontrolled T2DM cause many complications such as neurological diseases and lower limb amputations, in addition to other health problems. Insulin resistance and low-grade chronic inflammation in adipose tissue is a characteristic of people with obesity and diabetes. In fact, T2DM is inflammatory cytokines are involved in the pathophysiology of this disease. About 1.4 million Iraqis suffer from diabetes. In this study, some clinical characteristics and parameters level sixty patients with T2DM (32 male and 28 female) and thirty as controls were quantified. There are no significant differences between patients and controls in both age and BMI. There are expected significant increase in FBG, HbA1c and HOMA-IR in T2DM patients than healthy groups. There is a significant decline (p = 0.003) in serum IL-24 level in T2DM patients as compared with the controls. The low level of interleukin-24 in patients with T2DM has serious consequences. Despite the neuropathies in patients and the disease-causing nerve damage and non-healing of wounds, the decrease of this interleukin leads to may decrease in the patients’ immunity. The skin acts as a barrier against harmful microbes on several levels: colonization, pH, mechanical/physical and immunological IL-24 significant decrease (p < 0.001) in patients with type 2 diabetes and those who suffer from diabetic foot (DF) ulcers in particular affects the healing of ulcers and poorly in wound healing. There is a significant decline (p = 0.03) in serum IL-29 level in T2DM patients as compared with controls. The decreased IL-29 levels in patients may be defect in immune system particularly patients with T2DM, where IL-29 is secreted from T-helper that affects keratinocytes and activated against viruses. In contrast, an IL-29 level decrease in patients with DF ulcers induce an inflammatory response.
{"title":"IL-24 AND IL-29 IN T2DM WITH AND WITHOUT DIABETIC FOOT ULCERS","authors":"Eswadi Aya Refat, AL-Hellawi Zainab Hussein","doi":"10.47743/achi-2022-2-0005","DOIUrl":"https://doi.org/10.47743/achi-2022-2-0005","url":null,"abstract":": Type 2 Diabetes Mellitus (T2DM) is a common chronic metabolic disease that spread worldwide. Uncontrolled T2DM cause many complications such as neurological diseases and lower limb amputations, in addition to other health problems. Insulin resistance and low-grade chronic inflammation in adipose tissue is a characteristic of people with obesity and diabetes. In fact, T2DM is inflammatory cytokines are involved in the pathophysiology of this disease. About 1.4 million Iraqis suffer from diabetes. In this study, some clinical characteristics and parameters level sixty patients with T2DM (32 male and 28 female) and thirty as controls were quantified. There are no significant differences between patients and controls in both age and BMI. There are expected significant increase in FBG, HbA1c and HOMA-IR in T2DM patients than healthy groups. There is a significant decline (p = 0.003) in serum IL-24 level in T2DM patients as compared with the controls. The low level of interleukin-24 in patients with T2DM has serious consequences. Despite the neuropathies in patients and the disease-causing nerve damage and non-healing of wounds, the decrease of this interleukin leads to may decrease in the patients’ immunity. The skin acts as a barrier against harmful microbes on several levels: colonization, pH, mechanical/physical and immunological IL-24 significant decrease (p < 0.001) in patients with type 2 diabetes and those who suffer from diabetic foot (DF) ulcers in particular affects the healing of ulcers and poorly in wound healing. There is a significant decline (p = 0.03) in serum IL-29 level in T2DM patients as compared with controls. The decreased IL-29 levels in patients may be defect in immune system particularly patients with T2DM, where IL-29 is secreted from T-helper that affects keratinocytes and activated against viruses. In contrast, an IL-29 level decrease in patients with DF ulcers induce an inflammatory response.","PeriodicalId":6958,"journal":{"name":"Acta Chemica Iasi","volume":"63 1","pages":""},"PeriodicalIF":0.5,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74361043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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