{"title":"Image formation in three‐photon fluorescence microscopy","authors":"C. Sheppard","doi":"10.1002/1361-6374(199609)4:3<124::AID-BIO2>3.0.CO;2-2","DOIUrl":null,"url":null,"abstract":"Image formation in three-photon fluorescence microscopy is analysed. The point spread function, three-dimensional optical transfer function and axial resolution are considered. The results are generalized to multiphoton fluorescence. Three-photon fluorescence is particularly amenable to resolution improvement by use of pupil filters.","PeriodicalId":100176,"journal":{"name":"Bioimaging","volume":"102 3 Suppl 1","pages":"124-128"},"PeriodicalIF":0.0000,"publicationDate":"1996-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"16","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bioimaging","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1002/1361-6374(199609)4:3<124::AID-BIO2>3.0.CO;2-2","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 16
Abstract
Image formation in three-photon fluorescence microscopy is analysed. The point spread function, three-dimensional optical transfer function and axial resolution are considered. The results are generalized to multiphoton fluorescence. Three-photon fluorescence is particularly amenable to resolution improvement by use of pupil filters.
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