A Novel Bacterial Culture Media: Fruit Waste Agar

Abstract

The development of microbiology began in the 19th century with the invention of the culture medium. Bacterial culture was the first method to study human microbiota in 1860. Louis pasteur was the first to propagate bacteria on culture media. The culture media provides the essential nutrients such as carbohydrates, protein, vitamins and some growth factors for the proper development of bacteria. Nowadays, these growth medias are prepared by different expensive chemical ingredients in laboratories for research experiments, which ultimately makes our experiments expensive. Instead of using high-cost culture media, fruit waste material could prove to be a good alternate source for the production of low-cost media. The waste generated in household practices and kitchen includes vegetables and fruit waste. It is waste that almost every house generates every day. It can serve as a good source of nutrients and vitamins for in microorganisms. Hence these materials can be used to formulate solid media for the growth of bacteria. In the current study waste material like mango peels, banana peels, lemon peels and ground nut shell have been included to formulate the media. This material was collected from kitchen waste and vegetable market. Comparing the growth of organism (lactobacillus bulgaricus) standard commercial media and fruit waste with agar, it was found that the media prepared from fruit waste serves as a good and inexpensive source of nutrients for many bacteria. Thus, it can good be feather and used commercially for isolation and cultivation of various microorganisms.