[In vitro Cultivation of Functioning Passaged Ciliated Epithelium for Trachea Tissue Engineering].

Abstract

BACKGROUND Currently all tissue engineered trachea transplants had no ciliated epithelium until transplantation, and long-term temporary lack of mucociliary clearance leads to patients' condition decline and reduced life quality in postoperative period. So, the need for a better cultivation method and studying ciliated epithelium growth characteristics in cell culture increased rapidly. OBJECTIVE The aim of our study was to investigate cultivation offunctionally complete passaged ciliated epitheliumfor trachea tissue engineering. METHODS Human ciliated epithelium isolated from intraoperative bioptate was used for culturing in the special complex medium with morphological and functional characteristics evaluation. RESULTS Ciliated epithelial cell-groups were obtained by culturing in the special complex medium. Generated cell-groups had ciliary activity and showed well-coordinated movement with functional characteristics similar to native epithelial tissue. The basic parameters of cell-activity were studied. CONCLUSION Thus our study provides a new insight for the problem of ciliated epithelium in vitro culturing as well as developing the optimal laboratory method.