Comparison of Two Analytical Methods for Detecting (1-3)-β-D-Glucan in Pure Fungal Cultures and in Home Dust Samples

The Open Allergy Journal Pub Date : 2008-07-09 DOI:10.2174/1874838400801010026

Y. Iossifova, T. Reponen, M. Daines, L. Levin, G. Hershey

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引用次数: 22

Abstract

There are two methods available for the analysis of (1-3)-￻ -D-glucan: the Limulus Amebocyte Lysate assay (LAL) and the inhibition Enzyme Immunoassay (EIA). The aim of this study was to compare the accuracy and specificity of these two methods in detecting eight alpha and beta-glucan standards, and their sensitivity for the analysis of (1-3)-￻ - D-glucan content of common indoor fungal species and indoor dust samples. The results show that the LAL assay is more accurate, specific, and sensitive in measuring linear and branched ￻ -D-glucans than the EIA. The greatest LAL-analyzed (1-3)-� -D-glucan content per spore (241 pg/spore) was found with E. nigrum, which also had the largest spore size (28 μm). The biomass-normalized (1-3)-� -D-glucan content of fungal spores from pure cultures was within similar range with the two assays but no correlation was found between the results from the two assays. In contrast, there was a significant correlation between the EIA and LAL-measured (1-3)-� -D-glucan concentrations (� g/m 2 of floor area) in field dust sam- ples.

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纯真菌培养物与家庭粉尘样品中(1-3)-β- d -葡聚糖两种检测方法的比较

目前有两种分析(1-3)-￻- d -葡聚糖的方法:鲎试剂溶栓法(LAL)和抑制酶免疫法(EIA)。本研究的目的是比较这两种方法检测8种α -葡聚糖和β -葡聚糖标准的准确性和特异性，以及它们对室内常见真菌和室内粉尘样品中(1-3)-￻- d -葡聚糖含量的敏感性。结果表明，与EIA相比，LAL法在测量线性和支链￻- d -葡聚糖方面具有更高的准确性、特异性和敏感性。l - 1-3 - d -葡聚糖含量最高的是E. nigrum，其孢子大小也最大(28 μm)。纯培养真菌孢子的生物量归一化(1-3)- d -葡聚糖含量与两种测定结果在相似范围内，但两种测定结果之间没有相关性。相比之下，在田间粉尘样品中，EIA与lal测量的(1-3)- α - d -葡聚糖浓度(-g / m2)之间存在显著相关性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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The Open Allergy Journal

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