In vitro Anti-microbial Activity and Wound Healing Evaluation of Acacia Gum Arabia Aqueous Cream

Abstract

Objective: The aim of the present study was to characterize the antimicrobial properties of Acacia Gum Arabia crude extracts and Acacia Gum Arabia aqueous cream against certain selected quality control microorganisms. Methodology: A serial concentrations were prepared 2.5%, 5%, 10%, 15%, and 20% of Acacia Gum Arabia. An aqueous cream with 5% concentration of Acacia Gum Arabia were prepared and evaluated. A quality control tests were conducted to check the stability, organoleptic properties and pH of the cream. An in vitro evaluation against Gram positive bacteria (Bacillus subtilis and Staphylococcus aureus), Gram negative bacteria (Escherichia coli, and Pseudomonas aeroginosa) and yeast (Candida albicans). The Minimum Inhibitory Concentration (MIC) were determined. Results: Out of sex (6) tested microorganisms, it is found that 5% is the optimum concentration that most of the microorganisms are reported to shows zone of inhibitions. A further experiments were conducted for minimum inhibitory concentrations (MIC). The MIC values are found to be Proteus 1.25%, Shigella 2.5-1.5%, Staphylococcus epidermidis 1.25%, and Klebsiella 2.5%. The quality control tests for Acacia Gum Arabia cream reveals that for the spreadability test the measured diameter of each types (spreadability), in which the average diameter of aqueous cream and acacia cream ware 2.4 and 2.2 ± 0.14, respectively. The topical treatment with Acassia gum cream showed significant healing effect on excision wounds and demonstrated an important role in the inflammation process by increasing antioxidant enzyme activities, thereby accelerating the wound healing process and reducing tissue injury. Conclusion: it can be concluded that Acacia Gum Arabia demonstrate an antibacterial activity for certain types of bacterias. And the Acacia Gum Arabia cream can be used as an antibacterial cream.