{"title":"Isolation of Nervous Necrosis Virus from Hybrid Grouper (Epinephelus fuscoguttatus♀ × Epinephelus lanceolatus♂) Cultured in Guangxi, China","authors":"Qing Yu, Mingzhu Liu, Shina Wei, Hehe Xiao, Siting Wu, Xianling Qin, Deqiang Shi, Siqiao Li, Taixia Wang, Pengfei Li","doi":"10.3147/JSFP.54.16","DOIUrl":null,"url":null,"abstract":"― Outbreaks of suspected viral nervous necrosis emerged in cultured groupers in Guangxi, China in August 2018. Histopathology showed severe vacuolation in the brain tissue of the diseased juvenile hybrid grouper ( Epinephelus fuscoguttatus ♀ × Epinephelus lanceolatus ♂ ). A nervous necrosis virus (NNV) was isolated from pooled sample of the brain and eyes using a grouper cell line EAGB. Cytopathic effect with multiple cytoplasmic vacuolation cells developed in EAGB cells. The coat protein gene sequence of the isolate (GNNV-Gx) showed that it belonged to RGNNV genotype and was most closely related to that of a tiger grouper NNV registered in GenBank. The infectivity of the isolate decreased remarkably at pH 3 and pH 10 and was susceptible to 60 ° C.","PeriodicalId":51052,"journal":{"name":"Fish Pathology","volume":"9 1","pages":""},"PeriodicalIF":0.6000,"publicationDate":"2019-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"14","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Fish Pathology","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.3147/JSFP.54.16","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"FISHERIES","Score":null,"Total":0}
引用次数: 14
Abstract
― Outbreaks of suspected viral nervous necrosis emerged in cultured groupers in Guangxi, China in August 2018. Histopathology showed severe vacuolation in the brain tissue of the diseased juvenile hybrid grouper ( Epinephelus fuscoguttatus ♀ × Epinephelus lanceolatus ♂ ). A nervous necrosis virus (NNV) was isolated from pooled sample of the brain and eyes using a grouper cell line EAGB. Cytopathic effect with multiple cytoplasmic vacuolation cells developed in EAGB cells. The coat protein gene sequence of the isolate (GNNV-Gx) showed that it belonged to RGNNV genotype and was most closely related to that of a tiger grouper NNV registered in GenBank. The infectivity of the isolate decreased remarkably at pH 3 and pH 10 and was susceptible to 60 ° C.
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