Obtaining viable Azospirillum brasilense SR80 cells encapsulated in alginate hydrogel

A. V. Kovrizhnikov, T. Pylaev, A. M. Zaharevich, S. Konnova, M. Kupryashina
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Abstract

Significant researches aimed at the greening of agro-industrial production are focused on obtaining immobilized bacterial preparations with preserved proliferative function and metabolic activity. Herein, we investigated the possibility of bacteria of the genus Azospirillum to be immobilized in Ca-alginate beads. A. brasilense SR80 cells, encapsulated in an alginate hydrogel, were obtained using the “soft” immobilization method based on physical binding. We demonstrated the retained respiratory activity and growth ability of the bacteria during immobilization, thus confirming the advantageous prospects of alginate templates for azospirilla encapsulation.
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用海藻酸盐水凝胶包封可活的巴西氮螺旋菌SR80细胞
在农业工业生产的绿色化方面,重要的研究集中在获得具有保留增殖功能和代谢活性的固定化细菌制剂上。在此,我们研究了氮螺旋菌属细菌在海藻酸钙珠中固定化的可能性。采用基于物理结合的“软”固定化方法,将巴西螺SR80细胞包封在海藻酸盐水凝胶中。我们证明了细菌在固定化过程中保持了呼吸活性和生长能力,从而证实了海藻酸盐模板用于偶氮螺旋藻包封的有利前景。
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