{"title":"An Overview on the Main Technical Processes of Restriction-Site Associated DNA Sequencing (RAD-Seq)","authors":"Liao QingQing, Momo Li, Jiefei Qiu, Chen Fenfen, Chen Min, Qilin Wang, Guangyu Li, Guo Aiwei","doi":"10.12783/DTMSE/AMEME2020/35548","DOIUrl":null,"url":null,"abstract":"Restriction site-associated DNA sequencing (RAD-seq) is a critical enabling genotyping method based on whole-genome restriction sites developed on the basis of second-generation sequencing and nowadays is widely applicable to a variety of biological problems in many species where traditional cytogenetic methods have failed. As sequencing costs have dropped, RAD-Seq has moved from being primarily a SNP discovery tool to being a method for high-throughput genotyping of populations. In this paper, the main technical processes of RAD-seq has been briefly summarized and great potential will been hold to clarify more development and application in researches.","PeriodicalId":11124,"journal":{"name":"DEStech Transactions on Materials Science and Engineering","volume":"9 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2021-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"DEStech Transactions on Materials Science and Engineering","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.12783/DTMSE/AMEME2020/35548","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Restriction site-associated DNA sequencing (RAD-seq) is a critical enabling genotyping method based on whole-genome restriction sites developed on the basis of second-generation sequencing and nowadays is widely applicable to a variety of biological problems in many species where traditional cytogenetic methods have failed. As sequencing costs have dropped, RAD-Seq has moved from being primarily a SNP discovery tool to being a method for high-throughput genotyping of populations. In this paper, the main technical processes of RAD-seq has been briefly summarized and great potential will been hold to clarify more development and application in researches.