PET-CT tracing and fluorescence imaging to monitor the colonization and distribution of combined transplantation of islets and BMSC

Ling-ling Wei, Shi Jing, Feng Tianhang, Chunyou Lai, Zhang Tianying, Yu-tong Yao, S. Deng, Xiaolun Huang
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引用次数: 1

Abstract

Objective To further observe the efficacy of combined transplantation of islet and bone marrow mesenchymal stem cells (BMSC) in diabetic rats, PET-CT was used to trace cells in vivo to determine the homing and distribution of cells in vivo. Methods Streptozotocin (STZ)was used to construct a rat model of diabetes mellitus. BMSC could be isolated and cultured by full adherence method; islets were isolated by collagenase; Islets and BMSC were labeled with 18F-FDG in vitro. Diabetic rats were randomly divided into 4 groups, 15 rats in each group: A, Control group; B, Stem cell transplantation group; C, Islet Transplantation group; D, Combined transplantation group, a total of four groups, all transplanted through portal vein, PET-CT tracing the distribution of cells transplanted into the body.7 days after transplantation, the livers of each group were taken, and the homing and distribution of transplanted cells were detected by immunofluorescent staining.The SUV was calculated by the analysis of variance of random block, and the difference between groups was compared by t-test. Results PET-CT results showed that BMSC were mainly distributed uniformly in the right liver, and the islets of the pancreas were mainly clustered in terminal branches of hepatic portal vein, and BMSC were around the islets of pancreas, but there was no obvious development in the liver of the control group. Conclusions PET-CT can directly reveal the distribution of islets and BMSC in liver after transplantation through portal vein. Key words: Islet transplantation; Bone marrow mesenchymal stem cell; Positron emission tomography and computed tomography
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PET-CT示踪和荧光成像监测胰岛与骨髓间充质干细胞联合移植的定植和分布
目的为进一步观察胰岛骨髓间充质干细胞(BMSC)联合移植治疗糖尿病大鼠的疗效,采用PET-CT对体内细胞进行追踪,确定细胞在体内的归巢和分布情况。方法采用链脲佐菌素(STZ)建立糖尿病大鼠模型。全贴壁法可分离培养BMSC;用胶原酶分离胰岛;用18F-FDG体外标记胰岛和骨髓间充质干细胞。将糖尿病大鼠随机分为4组,每组15只:A、对照组;B,干细胞移植组;C,胰岛移植组;D、联合移植组,共4组,均经门静脉移植,PET-CT追踪移植体内细胞分布。移植后7 d取各组肝脏,免疫荧光染色检测移植细胞归巢及分布情况。SUV采用随机块方差分析计算,组间差异采用t检验比较。结果PET-CT结果显示,BMSC主要均匀分布于右肝,胰腺胰岛主要聚集在肝门静脉终末支,胰腺胰岛周围有BMSC,对照组肝脏未见明显发展。结论PET-CT可直接显示经门静脉肝移植后胰岛及骨髓间充质干细胞的分布。关键词:胰岛移植;骨髓间充质干细胞;正电子发射断层扫描和计算机断层扫描
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