Effects of Coconut, Groundnut and Tigernut Milk-Based Extenders on Fresh and Chilleduda Ram Semen in Maiduguri, Nigeria

M. Bamanga, A. Abba, A. Mustapha, M. M. Bukar, M. Waziri, V. Maina, A. Y. Ribadu, J. D. Amin
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Abstract

This study was conducted to determine the effects of coconut, groundnut and Tigernut milk based extenders on some characteristics of Uda ram semen in Maiduguri, Nigeria. A total of 96 semen samples were collected using electro-ejaculator from six Uda rams, twice a week, for 8 weeks. Semen was pooled and divided into 4 aliquots. One aliquot was diluted with Oviplus® solution and egg yolk (Oviplus® + egg yolk) and served as control. The other 3 aliquots were extended with coconut, groundnut and tigernut milk-based extenders respectively. The samples were chilled to 5°C and evaluated for individual motility, livability and morphologic abnormalities. These evaluations were made immediately, at 24, 48 and at 72 hours post extension. The average semen volume, individual motility, livability, morphologic abnormalities, pH and concentration of semen analyzed immediately after collection were 1.35 ± 0.1 ml, 80.9 ± 1.5 (%), 75.8 ± 2.0 (%), 8.0 ± 0.8 (%), 6.9 ± 0.1 and 3.2 ± 0.2 (x109/ml) respectively. Furthermore, it was found that coconut, ground nut and tiger nut milkbased extenders maintained good semen quality of Uda rams till 48 hours post extension when chilled at 5°C and that the tiger nut milk based extenders have better semen preservative ability than the coconut and the ground nut milk based extenders.
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椰子、花生和虎坚果乳基增稠剂对尼日利亚迈杜古里新鲜公羊和子母羊精液的影响
在尼日利亚迈杜古里地区,研究了椰子、花生和虎坚果乳为基础的膨化剂对乌达公羊精液某些特性的影响。用电射精器采集6只乌达公羊96份精液样本,每周2次,连续8周。收集精液,分成4等份。用Oviplus®溶液和蛋黄(Oviplus®+蛋黄)稀释其中一份作为对照。另外3个等价物分别添加椰子、花生和虎坚果乳基增稠剂。将样品冷冻至5°C,评估个体运动性、宜居性和形态异常。这些评价是在延长后24、48和72小时立即进行的。采集后即刻分析的平均精液体积、个体活力、存活率、形态异常、pH值和精液浓度分别为1.35±0.1 ml、80.9±1.5(%)、75.8±2.0(%)、8.0±0.8(%)、6.9±0.1和3.2±0.2 (x109/ml)。此外,在5℃冷藏条件下,以椰子、坚果粉和虎坚果乳为基础的膨化剂在延长48 h后仍能保持良好的雄田公羊精液质量,并且虎坚果乳为基础的膨化剂比椰子和坚果粉为基础的膨化剂具有更好的精液保存能力。
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