Laser speckle spectroscopy—a new method for using small swimming organisms as biomonitors

J. Cole, M. Tinker
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引用次数: 4

Abstract

A novel method has been devised for the study of swimming organisms by using speckle patterns produced by their scattering of coherent laser light. The speckle patterns show fluctuations in space and time which may be correlated with the activity of the organisms. The fluctuations give an immediate indication of mobility and a more detailed analysis of the frequency spectrum of the speckle fluctuations shows characteristic resonance-like features which are specific to the organism. The speckle patterns produced by several protozoans, including Paramecium bursaria, Entosiphon sulcatum, and by the alga Chlamydomonas reinhardii and the rotifer Brachionus calyciflorus have been studied. Laser speckle spectroscopy (LSS) allows a rapid non-invasive monitoring of the activity of the organisms and could find application in ecotoxicity studies and environmental biomonitoring. The results presented here are the first reports of LSS and its use in this way and demonstrate its viability and potential for further development.
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激光散斑光谱——一种利用小型游动生物作为生物监测仪的新方法
一种新的方法已经被设计出来用于研究游泳生物,利用它们的相干激光散射产生的斑点图案。斑点图案显示了时空的波动,这可能与生物体的活动有关。这些波动可以立即显示出迁移率,对散斑波动的频谱进行更详细的分析,可以显示出生物体特有的特征共振。本文研究了几种原生动物,包括滑囊草履虫、沟内虹吸、reinhardii衣藻和萼花臂轮虫所产生的斑点图案。激光散斑光谱(LSS)可以对生物活性进行快速无创监测,在生态毒性研究和环境生物监测中具有广泛的应用前景。本文介绍的结果是LSS及其以这种方式使用的首次报告,并展示了其可行性和进一步发展的潜力。
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