DETERMINATION of TOTAL FLAVONOID LEVELS on LEAF STALKS ETHANOL EXTRACT of TARO (Colocasia esculenta[L.]Schott)

Novena Yety Lindawati
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引用次数: 2

Abstract

Taro is plant that has a pseudostalk,cylindrical and light brown bulbs. The shape of leaf is the heart in length. Leaf stalk of taro contains are saponins, flavonoids, tanins, alkaloids, steroids and terpenoids. Leaf stalk of taro can be used as an alternative medicines wound and antioxidants. Flavonoids have an important role in the biological activity of taro leaf stalk. This research aims to determine the flavonoid and total flavonoids content in the taro leaf stalk extract. The extraction is done using maceration method with 70% ethanolas solvent. The extract which is got is used for qualitative and quantitative analysis. Quercetin used as a standard solution. Quantitative analysis using UV-Vis Spectrophotometry on a wavelength of 435.5 nm and operating time at the 29th minute. The results of qualitative test showed that the extract was positive flavonoids. The average concentrations of total flavonoids was 10,2223mg QE/gram extract with %CV value of 0,3051%.
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芋(Colocasia esculenta)叶片乙醇提取物中总黄酮含量的测定
芋头是一种植物,有假茎,圆柱形和浅棕色的球茎。叶子的形状是心脏的长度。芋头叶柄中含有皂苷、黄酮类化合物、单宁、生物碱、类固醇和萜类化合物。芋头叶柄可作为创面和抗氧化剂的替代药物。类黄酮在芋头叶柄的生物活性中具有重要作用。本研究旨在测定芋头叶柄提取物中总黄酮和类黄酮的含量。采用70%乙醇溶剂浸渍法提取。所得提取物用于定性和定量分析。槲皮素作为标准溶液。定量分析采用紫外可见分光光度法,波长为435.5 nm,操作时间为29分钟。定性分析结果表明,提取液中黄酮类化合物呈阳性。总黄酮平均浓度为102223 mg QE/g, %CV值为0.03051%。
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