Molecular analysis of tetracycline resistant gene in gram-negative bacteria isolated from dairy farms

{"title":"Molecular analysis of tetracycline resistant gene in gram-negative bacteria isolated from dairy farms","authors":"","doi":"10.47262/6.2.20200724","DOIUrl":null,"url":null,"abstract":"The present study was conducted from April 2019 to June 2019 in order to detect the tetracycline A resistant Gene in gram negative bacteria. A total of 40 buffalo's milk samples were collected randomly by aseptic technique, brought to laboratory. They were inoculated on Blood and MacConkey agars and then incubated at 37°C for 24 hours whereby growth of colonies were further confirmed with catalase test, Coagulase test, Oxidase test, Gram staining and API 20 E kit. Bacterial DNA was isolated using the boiling method. The Tet A gene (210 bp) was amplified in thermal cycler and run on 1.8-gram agarose gel with 50 kb ladder. The most predominant bacterial colonies observed were of Escherichia coli (10 (33.3%) followed by Klebsiella pneumonia 5 (16.7%), Klebsiella spp. 5 (16.7 %), Pseudomonas spp. 10 (33.3%) and prevalence of tetracycline A gene was 8 (26.7%).","PeriodicalId":9154,"journal":{"name":"Biomedical Letters","volume":"87 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2020-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biomedical Letters","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.47262/6.2.20200724","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

Abstract

The present study was conducted from April 2019 to June 2019 in order to detect the tetracycline A resistant Gene in gram negative bacteria. A total of 40 buffalo's milk samples were collected randomly by aseptic technique, brought to laboratory. They were inoculated on Blood and MacConkey agars and then incubated at 37°C for 24 hours whereby growth of colonies were further confirmed with catalase test, Coagulase test, Oxidase test, Gram staining and API 20 E kit. Bacterial DNA was isolated using the boiling method. The Tet A gene (210 bp) was amplified in thermal cycler and run on 1.8-gram agarose gel with 50 kb ladder. The most predominant bacterial colonies observed were of Escherichia coli (10 (33.3%) followed by Klebsiella pneumonia 5 (16.7%), Klebsiella spp. 5 (16.7 %), Pseudomonas spp. 10 (33.3%) and prevalence of tetracycline A gene was 8 (26.7%).
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
奶牛场革兰氏阴性菌中四环素耐药基因的分子分析
本研究于2019年4月至2019年6月进行,目的是检测革兰氏阴性菌中四环素A耐药基因。采用无菌法随机采集40份水牛乳样品,送入实验室。分别接种于Blood琼脂和MacConkey琼脂上,37℃孵育24小时,通过过氧化氢酶、凝固酶、氧化酶、革兰氏染色和API 20e试剂盒进一步证实菌落的生长。采用煮沸法分离细菌DNA。在热循环器中扩增Tet A基因(210 bp),在1.8 g琼脂糖凝胶上以50 kb阶梯运行。结果显示,主要菌落为大肠埃希菌(10个)(33.3%),其次为肺炎克雷伯菌5(16.7%)、克雷伯菌5(16.7%)、假单胞菌10(33.3%),四环素A基因感染率为8个(26.7%)。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
自引率
0.00%
发文量
0
期刊最新文献
Incidence and risk factors associated with peptic ulcer in different cities of Punjab, Pakistan Human monkeypox virus: A review on the globally emerging virus Recent progress in the application of biodegradable metal implants Recent progress in the application of biodegradable metal implants Bioimpedance spectroscopy characterization of Myalgic Encephalomyelitis/ Chronic Fatigue Syndrome (ME/CFS) peripheral blood mononuclear cells
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1