Pub Date : 2024-07-11DOI: 10.47262/bl/10.1.20240416
Peptic ulcers are severe digestive tract mucosal lesions. Worldwide, peptic ulcer disease (PUD) increases medical costs and morbidity. PUD is rising in Islamabad, Rawalpindi, and Karachi due to lifestyle and changes in diet. PUD is linked to drug and alcohol use, smoking, lack of exercise, and emotional stress. Infection with Helicobacter pylori, lack of sleep, and obesity also raise ulcer risk. This study examined the lack of PUD research in three main cities of Punjab (Bahawalpur, Multan, and Lahore). These populations were studied for PUD incidence, complications, risk factors, correlations with other diseases, medications, and blood group linkages. Data was collected by a cross-sectional study from November 2022 to June 2023 on peptic ulcer symptoms in participants aged 11 and above. Questionnaires collected demographic, medical, lifestyle, and nutritional data. Heart rate, blood pressure, and H. pylori status were checked. SPSS 25.0 was used to analyze data. Out of 200 participants, 47.5% were men and 52.5% women. There is no correlation between age, gender, or peptic ulcer prevalence in men or women. The sample comprised more rural than urban individuals. Both men and women with peptic ulcers had an O+ blood group. Women had more fever and belly pain. This study shows the prevalence and risk factors of peptic ulcers in urban Pakistan, highlighting the need for prevention and treatment. These findings highlight PUD across genders and suggest future research should consider sample size and self-reporting.
{"title":"Incidence and risk factors associated with peptic ulcer in different cities of Punjab, Pakistan","authors":"","doi":"10.47262/bl/10.1.20240416","DOIUrl":"https://doi.org/10.47262/bl/10.1.20240416","url":null,"abstract":"Peptic ulcers are severe digestive tract mucosal lesions. Worldwide, peptic ulcer disease (PUD) increases medical costs and morbidity. PUD is rising in Islamabad, Rawalpindi, and Karachi due to lifestyle and changes in diet. PUD is linked to drug and alcohol use, smoking, lack of exercise, and emotional stress. Infection with Helicobacter pylori, lack of sleep, and obesity also raise ulcer risk. This study examined the lack of PUD research in three main cities of Punjab (Bahawalpur, Multan, and Lahore). These populations were studied for PUD incidence, complications, risk factors, correlations with other diseases, medications, and blood group linkages. Data was collected by a cross-sectional study from November 2022 to June 2023 on peptic ulcer symptoms in participants aged 11 and above. Questionnaires collected demographic, medical, lifestyle, and nutritional data. Heart rate, blood pressure, and H. pylori status were checked. SPSS 25.0 was used to analyze data. Out of 200 participants, 47.5% were men and 52.5% women. There is no correlation between age, gender, or peptic ulcer prevalence in men or women. The sample comprised more rural than urban individuals. Both men and women with peptic ulcers had an O+ blood group. Women had more fever and belly pain. This study shows the prevalence and risk factors of peptic ulcers in urban Pakistan, highlighting the need for prevention and treatment. These findings highlight PUD across genders and suggest future research should consider sample size and self-reporting.","PeriodicalId":9154,"journal":{"name":"Biomedical Letters","volume":"55 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141658163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-05DOI: 10.47262/bl/10.1.20242161
Monkeypox is a contagious complaint that affects both mortal and beast health and has lately come under the attention of all worlds. A genomic to developments in DNA sequencing, the genomic chart of the contagion has been known, which offers perceptivity into its elaboration and possible modes of transmission across different species. Understanding the complex mechanisms and studying the transmission of monkeypox is pivotal for disseminating the complaint’s spread from beast sources to mortal populations. Global frequency patterns demonstrate the complex connections between source hosts, vectors, and susceptible populations, and the deficit of exploration in Pakistan permits further disquisition into the possible public health counteraccusations. It's matter of great significance to completely explore the inheritable and antigenic parcels of this contagion, with its strong correlation with the etiology of monkeypox. PCR has proven to be a tool for accurate identification in the ongoing fight against this contagious disease. The variety of clinical signs and symptoms, which can vary from mild fever to severe lymphadenopathy, highlights the critical need for effective opinion and treatment strategies. Also, the maturity of available treatment options presently corresponds of probative care and antiviral specifics. Further exploration and cooperative sweats are necessary to increase our understanding and develop feasible therapeutics. This discussion highlights the need for a comprehensive plan to lessen the mischievous goods of monkeypox on the health of people and creatures. Beforehand discovery, visionary surveillance, and substantiation-grounded operation strategies must be put into practice.
猴痘是一种影响人类和野兽健康的传染性疾病，最近受到了全世界的关注。随着 DNA 测序技术的发展，人们已经知道了这种传染病的基因组图谱，这为了解其详细情况和在不同物种间的可能传播方式提供了线索。了解猴痘的复杂机制并研究其传播方式，对于将猴痘从野兽传染源传播到人类至关重要。全球频率模式显示了源宿主、病媒和易感人群之间的复杂联系，而巴基斯坦缺乏这方面的探索，因此可以进一步研究可能的公共卫生对策。这种传染病与猴痘的病因学密切相关，因此全面探索这种传染病的可遗传性和抗原性是非常重要的。事实证明，PCR 是目前抗击这种传染病的准确鉴定工具。临床症状和体征多种多样，从轻微发热到严重淋巴结肿大不等，这凸显了对有效意见和治疗策略的迫切需要。此外，现有治疗方案的成熟度目前与感化治疗和抗病毒特异性治疗相对应。有必要进行进一步的探索和合作，以加深我们的理解并开发可行的疗法。以上讨论强调了制定全面计划的必要性，以减少猴痘对人类和生物健康的危害。事先发现、有远见的监控和有依据的行动策略必须付诸实践。
{"title":"Human monkeypox virus: A review on the globally emerging virus","authors":"","doi":"10.47262/bl/10.1.20242161","DOIUrl":"https://doi.org/10.47262/bl/10.1.20242161","url":null,"abstract":"Monkeypox is a contagious complaint that affects both mortal and beast health and has lately come under the attention of all worlds. A genomic to developments in DNA sequencing, the genomic chart of the contagion has been known, which offers perceptivity into its elaboration and possible modes of transmission across different species. Understanding the complex mechanisms and studying the transmission of monkeypox is pivotal for disseminating the complaint’s spread from beast sources to mortal populations. Global frequency patterns demonstrate the complex connections between source hosts, vectors, and susceptible populations, and the deficit of exploration in Pakistan permits further disquisition into the possible public health counteraccusations. It's matter of great significance to completely explore the inheritable and antigenic parcels of this contagion, with its strong correlation with the etiology of monkeypox. PCR has proven to be a tool for accurate identification in the ongoing fight against this contagious disease. The variety of clinical signs and symptoms, which can vary from mild fever to severe lymphadenopathy, highlights the critical need for effective opinion and treatment strategies. Also, the maturity of available treatment options presently corresponds of probative care and antiviral specifics. Further exploration and cooperative sweats are necessary to increase our understanding and develop feasible therapeutics. This discussion highlights the need for a comprehensive plan to lessen the mischievous goods of monkeypox on the health of people and creatures. Beforehand discovery, visionary surveillance, and substantiation-grounded operation strategies must be put into practice.","PeriodicalId":9154,"journal":{"name":"Biomedical Letters","volume":"9 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141385789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-14DOI: 10.47262/bl/10.1.20231211
With the accumulation of data, magnesium-based degradable metal, iron-based degradable metal and zinc-based degradable metal implantable interventional devices have entered the clinic or carried out human experimental studies, and the future prospects are promising. In this paper, the definition, biodegradability and biocompatibility criteria and their classification are reviewed, and the research status and unsolved scientific problems of magnesium-based degradable metals, iron-based degradable metals and zinc-based degradable metals are introduced, and the future development opportunities and challenges of degradable metals are prospected. With a deeper understanding of scientific issues such as mechanical adaptation, degradation adaptation and tissue adaptation of degradable metal implants, more new materials, new technologies and new methods of degradable metals will be developed in the future, so as to effectively realize the precise adaptation of the two events of degradable metal material degradation and body tissue repair in time and geometric space.
{"title":"Recent progress in the application of biodegradable metal implants","authors":"","doi":"10.47262/bl/10.1.20231211","DOIUrl":"https://doi.org/10.47262/bl/10.1.20231211","url":null,"abstract":"With the accumulation of data, magnesium-based degradable metal, iron-based degradable metal and zinc-based degradable metal implantable interventional devices have entered the clinic or carried out human experimental studies, and the future prospects are promising. In this paper, the definition, biodegradability and biocompatibility criteria and their classification are reviewed, and the research status and unsolved scientific problems of magnesium-based degradable metals, iron-based degradable metals and zinc-based degradable metals are introduced, and the future development opportunities and challenges of degradable metals are prospected. With a deeper understanding of scientific issues such as mechanical adaptation, degradation adaptation and tissue adaptation of degradable metal implants, more new materials, new technologies and new methods of degradable metals will be developed in the future, so as to effectively realize the precise adaptation of the two events of degradable metal material degradation and body tissue repair in time and geometric space.","PeriodicalId":9154,"journal":{"name":"Biomedical Letters","volume":"187 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139836761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-14DOI: 10.47262/bl/10.1.20231211
With the accumulation of data, magnesium-based degradable metal, iron-based degradable metal and zinc-based degradable metal implantable interventional devices have entered the clinic or carried out human experimental studies, and the future prospects are promising. In this paper, the definition, biodegradability and biocompatibility criteria and their classification are reviewed, and the research status and unsolved scientific problems of magnesium-based degradable metals, iron-based degradable metals and zinc-based degradable metals are introduced, and the future development opportunities and challenges of degradable metals are prospected. With a deeper understanding of scientific issues such as mechanical adaptation, degradation adaptation and tissue adaptation of degradable metal implants, more new materials, new technologies and new methods of degradable metals will be developed in the future, so as to effectively realize the precise adaptation of the two events of degradable metal material degradation and body tissue repair in time and geometric space.
{"title":"Recent progress in the application of biodegradable metal implants","authors":"","doi":"10.47262/bl/10.1.20231211","DOIUrl":"https://doi.org/10.47262/bl/10.1.20231211","url":null,"abstract":"With the accumulation of data, magnesium-based degradable metal, iron-based degradable metal and zinc-based degradable metal implantable interventional devices have entered the clinic or carried out human experimental studies, and the future prospects are promising. In this paper, the definition, biodegradability and biocompatibility criteria and their classification are reviewed, and the research status and unsolved scientific problems of magnesium-based degradable metals, iron-based degradable metals and zinc-based degradable metals are introduced, and the future development opportunities and challenges of degradable metals are prospected. With a deeper understanding of scientific issues such as mechanical adaptation, degradation adaptation and tissue adaptation of degradable metal implants, more new materials, new technologies and new methods of degradable metals will be developed in the future, so as to effectively realize the precise adaptation of the two events of degradable metal material degradation and body tissue repair in time and geometric space.","PeriodicalId":9154,"journal":{"name":"Biomedical Letters","volume":"81 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139776843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-06DOI: 10.47262/bl/9.2.20230220
Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS) is a disabling and chronic disease, importantly related to the current COVID-19 pandemic. Currently, there are no specific laboratory tests to directly diagnose ME/CFS. In this work, the use of impedance spectroscopy is studied as a potential technique for the diagnosis of ME/CFS. A specific device for the electrical characterization of peripheral blood mononuclear cells was designed and implemented. Impedance spectroscopy measurements in the range from 1 Hz to 500 MHz were carried out after the osmotic stress of the samples with sodium chloride solution at 1M concentration. The evolution in time after the osmotic stress at two specific frequencies (1.36 kHz and 154 kHz) was analyzed. The device showed its sensitivity to the presence of cells and the evolution of the osmotic processes. Higher values of impedance (around 15% for both the real and imaginary part) were measured at 1.36 kHz in ME/CFS patients compared to control samples. No significant difference was found between patient samples and control samples at 154 kHz. Results help to further understand the diagnosis of ME/CFS patients and the relation of their blood samples with bioimpedance measurements.
{"title":"Bioimpedance spectroscopy characterization of Myalgic Encephalomyelitis/ Chronic Fatigue Syndrome (ME/CFS) peripheral blood mononuclear cells","authors":"","doi":"10.47262/bl/9.2.20230220","DOIUrl":"https://doi.org/10.47262/bl/9.2.20230220","url":null,"abstract":"Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS) is a disabling and chronic disease, importantly related to the current COVID-19 pandemic. Currently, there are no specific laboratory tests to directly diagnose ME/CFS. In this work, the use of impedance spectroscopy is studied as a potential technique for the diagnosis of ME/CFS. A specific device for the electrical characterization of peripheral blood mononuclear cells was designed and implemented. Impedance spectroscopy measurements in the range from 1 Hz to 500 MHz were carried out after the osmotic stress of the samples with sodium chloride solution at 1M concentration. The evolution in time after the osmotic stress at two specific frequencies (1.36 kHz and 154 kHz) was analyzed. The device showed its sensitivity to the presence of cells and the evolution of the osmotic processes. Higher values of impedance (around 15% for both the real and imaginary part) were measured at 1.36 kHz in ME/CFS patients compared to control samples. No significant difference was found between patient samples and control samples at 154 kHz. Results help to further understand the diagnosis of ME/CFS patients and the relation of their blood samples with bioimpedance measurements.","PeriodicalId":9154,"journal":{"name":"Biomedical Letters","volume":"80 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135351640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-13DOI: 10.47262/bl/9.2.20230429
MicroRNAs (miRNAs) are a class of small, non-coding RNA molecules that have been shown to be involved in a wide range of biological processes, including cancer. miRNAs are known to regulate the expression of genes, and their dysregulation has been linked to the development of cancer. In recent years a great deal of attention is received by miRNAs due to their potential as biomarkers for cancer. Biomarkers are measurable indicators of a biological state, and they can be used to diagnose, monitor, and treat diseases. miRNAs can be detected in biological fluids such as blood and saliva. This makes them ideal candidates for early cancer detection and monitoring. We herein reviewed current methods for the isolation of circulating miRNAs. Provide the most recent update about clinical trials aiming at using miRNAs as biomarkers for cancer. Additionally, we highlighted some pitfalls that should be realized to take advantage of the massive potential of miRNAs as a cancer biomarker. However, the potential of miRNAs as cancer biomarkers is very promising but advancements in factors such as miRNA isolation methods, and the type of samples are critical to incorporate miRNA-based diagnostic and prognostic markers in modern-day treatment regimens for cancer. This review concludes that miRNAs have enormous clinical significance as cancer biomarkers and recommends carefully selecting methods for the isolation of miRNAs based on the type of sample, and the downstream applications to generate clinically relevant results.
{"title":"MicroRNAs: The next generation of cancer biomarkers","authors":"","doi":"10.47262/bl/9.2.20230429","DOIUrl":"https://doi.org/10.47262/bl/9.2.20230429","url":null,"abstract":"MicroRNAs (miRNAs) are a class of small, non-coding RNA molecules that have been shown to be involved in a wide range of biological processes, including cancer. miRNAs are known to regulate the expression of genes, and their dysregulation has been linked to the development of cancer. In recent years a great deal of attention is received by miRNAs due to their potential as biomarkers for cancer. Biomarkers are measurable indicators of a biological state, and they can be used to diagnose, monitor, and treat diseases. miRNAs can be detected in biological fluids such as blood and saliva. This makes them ideal candidates for early cancer detection and monitoring. We herein reviewed current methods for the isolation of circulating miRNAs. Provide the most recent update about clinical trials aiming at using miRNAs as biomarkers for cancer. Additionally, we highlighted some pitfalls that should be realized to take advantage of the massive potential of miRNAs as a cancer biomarker. However, the potential of miRNAs as cancer biomarkers is very promising but advancements in factors such as miRNA isolation methods, and the type of samples are critical to incorporate miRNA-based diagnostic and prognostic markers in modern-day treatment regimens for cancer. This review concludes that miRNAs have enormous clinical significance as cancer biomarkers and recommends carefully selecting methods for the isolation of miRNAs based on the type of sample, and the downstream applications to generate clinically relevant results.","PeriodicalId":9154,"journal":{"name":"Biomedical Letters","volume":"11 6 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135310103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-11DOI: 10.47262/bl/9.2.20230518
Noel Shamaun, M. I. Fareed, Keziah Shaheen, Muhammad Ameer Moaavia, Aksa Khalid, Sonana Nadeem, Sehrish Naz, M. Fareed, MA Moaavia
Adenosine deaminase (ADA) is a functional enzyme that transforms deoxyadenosine and adenosine into deoxyinosine and inosine respectively. ADA deficiency causes toxic purine degradation byproducts to build up in the body, which has a particularly negative impact on lymphocytes and results in adenosine deaminase-deficient severe combined immunodeficiency. Different in silico techniques including threading, ab initio and homology modeling for 3D structure prediction were applied for the prediction of ADA structures. Following the three-dimensional structure prediction analyses, an extensive computational assessment of all predicted structures for reliability was performed. The overall quality factor of the predicted ADA structures was observed 62.45% in the predicted 3D models. A Ramachandran plot was created, and 94.80% of the residues were found in the allowed and favored regions of the protein structure plot. The molecular docking analyses were performed in order to identify the potential therapeutic medication targets against ADA. The virtually examined molecules through a virtually high throughput screening may have the ability the regulation the ADA activity. The least binding energy was calculated through the molecular docking analyses and the energy values were observed -8.7 Kcal/mol. The binding residues (Lys-367, Glu-424, Asp-422, Phe-381, Ile-377, Ser-430 and Glu-374) were conserved in all the interactional analyses of the docked complexes. Finding the effective binding domain in a protein three-dimensional structure is crucial for understanding of its structural makeup and determining its functions.
{"title":"Computational 3D structure prediction followed by molecular docking to reveal the novel drug targets against ADA","authors":"Noel Shamaun, M. I. Fareed, Keziah Shaheen, Muhammad Ameer Moaavia, Aksa Khalid, Sonana Nadeem, Sehrish Naz, M. Fareed, MA Moaavia","doi":"10.47262/bl/9.2.20230518","DOIUrl":"https://doi.org/10.47262/bl/9.2.20230518","url":null,"abstract":"Adenosine deaminase (ADA) is a functional enzyme that transforms deoxyadenosine and adenosine into deoxyinosine and inosine respectively. ADA deficiency causes toxic purine degradation byproducts to build up in the body, which has a particularly negative impact on lymphocytes and results in adenosine deaminase-deficient severe combined immunodeficiency. Different in silico techniques including threading, ab initio and homology modeling for 3D structure prediction were applied for the prediction of ADA structures. Following the three-dimensional structure prediction analyses, an extensive computational assessment of all predicted structures for reliability was performed. The overall quality factor of the predicted ADA structures was observed 62.45% in the predicted 3D models. A Ramachandran plot was created, and 94.80% of the residues were found in the allowed and favored regions of the protein structure plot. The molecular docking analyses were performed in order to identify the potential therapeutic medication targets against ADA. The virtually examined molecules through a virtually high throughput screening may have the ability the regulation the ADA activity. The least binding energy was calculated through the molecular docking analyses and the energy values were observed -8.7 Kcal/mol. The binding residues (Lys-367, Glu-424, Asp-422, Phe-381, Ile-377, Ser-430 and Glu-374) were conserved in all the interactional analyses of the docked complexes. Finding the effective binding domain in a protein three-dimensional structure is crucial for understanding of its structural makeup and determining its functions.","PeriodicalId":9154,"journal":{"name":"Biomedical Letters","volume":"25 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78148136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-30DOI: 10.47262/bl/9.2.20230717
The human body and brain are affected by light both visually and non-visually. Light has extraordinary impact on large group of physiological capabilities, and encompass neuroendocrine regulation, sleep, alertness, cognition, and ocular reflexes, as well as phase-shifting and synchronization of the circadian framework. The blue light exposure is significant for keeping living organisms, cognitive performance prosperity and sharpness. The human eyes may suffer from excessive exposure of the blue light. The lack of light has a negative impact on sleep quality and alertness as well as mood, seasonal affective disorder and neurocognitive cycles. Early morning exposure to strong light delays the peak of melatonin production and alters cortisol, GH, PRL, and nocturnal vasopressin emission. Metabolic capabilities including the reducing levels of glucose resistance and diminished insulin sensitivity are horribly affected by night light exposure. Type 2 diabetes risk increases in an old populace due to the elevation in night light exposure. Ladies presented to night-light moves had sporadic monthly cycles that were much of the time related to dysmenorrhea and metabolic disorder insulin obstruction and liberation of glucose digestion. Estrus cycles, ovulation, sperm production, implantation, and the development of pregnancy are also affected by the desynchronizing effect of altered light signals on the circadian peripheral clocks in female and male conceptive tissues. DNA is harmed directly by UVB radiation. The present effort is to investigate and summarize the non-visual and alerting effect of light on the physiology of the human body.
{"title":"Non-visual and alerting impact of light on the physiology of human body","authors":"","doi":"10.47262/bl/9.2.20230717","DOIUrl":"https://doi.org/10.47262/bl/9.2.20230717","url":null,"abstract":"The human body and brain are affected by light both visually and non-visually. Light has extraordinary impact on large group of physiological capabilities, and encompass neuroendocrine regulation, sleep, alertness, cognition, and ocular reflexes, as well as phase-shifting and synchronization of the circadian framework. The blue light exposure is significant for keeping living organisms, cognitive performance prosperity and sharpness. The human eyes may suffer from excessive exposure of the blue light. The lack of light has a negative impact on sleep quality and alertness as well as mood, seasonal affective disorder and neurocognitive cycles. Early morning exposure to strong light delays the peak of melatonin production and alters cortisol, GH, PRL, and nocturnal vasopressin emission. Metabolic capabilities including the reducing levels of glucose resistance and diminished insulin sensitivity are horribly affected by night light exposure. Type 2 diabetes risk increases in an old populace due to the elevation in night light exposure. Ladies presented to night-light moves had sporadic monthly cycles that were much of the time related to dysmenorrhea and metabolic disorder insulin obstruction and liberation of glucose digestion. Estrus cycles, ovulation, sperm production, implantation, and the development of pregnancy are also affected by the desynchronizing effect of altered light signals on the circadian peripheral clocks in female and male conceptive tissues. DNA is harmed directly by UVB radiation. The present effort is to investigate and summarize the non-visual and alerting effect of light on the physiology of the human body.","PeriodicalId":9154,"journal":{"name":"Biomedical Letters","volume":"18 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89908899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-21DOI: 10.47262/bl/9.1.20230421
Signal transducer and activator of transcription 3 (STAT3) is a transcription factor, that contains a DNA-binding domain, N-terminal domain, and SH2 domain. The dysregulation of STAT3 activity has been associated with various diseases, such as chronic inflammation and autoimmune disorders. In cancer, STAT3 is often constitutively activated and promotes tumor cell survival, proliferation, and immune evasion. Various bioinformatics approaches were employed to predict the 3D structure of STAT3, followed by a comprehensive evaluation of the predicted model. 3D predicted structure of the target protein revealed an overall quality factor of 94. 45%. It was also observed through the Ramachandran plot that 1.26% residues of the predicted structure of STAT3 were present in the outlier region of the protein structure. Computational docking studies were done to identify the novel drug targets against STAT3. The screened compound via high throughput virtual screening may have the potential to regulate the activity of STAT3. The lowest binding energy of -8.7 Kcal/mol was observed. His-457, Tyr-456, Lys-488, Pro-487, Gln-326, Leu-459, Lys-244, Gln-247 conserved residues were observed. The structural insight and functional determination of STAT3 depend on the identification of the potent binding domain in protein 3D structure.
STAT3 (Signal transducer and activator of transcription 3)是一种转录因子，包含dna结合域、n端结构域和SH2结构域。STAT3活性的失调与多种疾病有关，如慢性炎症和自身免疫性疾病。在癌症中，STAT3经常被组成性激活，促进肿瘤细胞存活、增殖和免疫逃避。采用多种生物信息学方法预测STAT3的三维结构，然后对预测模型进行综合评估。三维预测的目标蛋白结构显示，总体质量因子为94。45%。通过Ramachandran图也观察到1.26%的预测结构残基存在于STAT3蛋白结构的离群区。通过计算对接研究来确定针对STAT3的新型药物靶点。通过高通量虚拟筛选筛选的化合物可能具有调节STAT3活性的潜力。最低结合能为-8.7 Kcal/mol。观察到His-457、Tyr-456、Lys-488、Pro-487、Gln-326、Leu-459、Lys-244、Gln-247等保守残基。STAT3的结构洞察和功能确定依赖于蛋白质三维结构中有效结合域的识别。
{"title":"Structural insights and computational molecular docking to explore novel therapeutic drug targets of STAT3","authors":"","doi":"10.47262/bl/9.1.20230421","DOIUrl":"https://doi.org/10.47262/bl/9.1.20230421","url":null,"abstract":"Signal transducer and activator of transcription 3 (STAT3) is a transcription factor, that contains a DNA-binding domain, N-terminal domain, and SH2 domain. The dysregulation of STAT3 activity has been associated with various diseases, such as chronic inflammation and autoimmune disorders. In cancer, STAT3 is often constitutively activated and promotes tumor cell survival, proliferation, and immune evasion. Various bioinformatics approaches were employed to predict the 3D structure of STAT3, followed by a comprehensive evaluation of the predicted model. 3D predicted structure of the target protein revealed an overall quality factor of 94. 45%. It was also observed through the Ramachandran plot that 1.26% residues of the predicted structure of STAT3 were present in the outlier region of the protein structure. Computational docking studies were done to identify the novel drug targets against STAT3. The screened compound via high throughput virtual screening may have the potential to regulate the activity of STAT3. The lowest binding energy of -8.7 Kcal/mol was observed. His-457, Tyr-456, Lys-488, Pro-487, Gln-326, Leu-459, Lys-244, Gln-247 conserved residues were observed. The structural insight and functional determination of STAT3 depend on the identification of the potent binding domain in protein 3D structure.","PeriodicalId":9154,"journal":{"name":"Biomedical Letters","volume":"90 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82456590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-13DOI: 10.47262/bl/9.2.20230408
Long noncoding RNA (lncRNA) has been recently revealed as a main regulatory molecule, implicating many cellular functions. Studies showed that lncRNA is abnormally expressed and involved in the progression and tumorigenesis of glioma. The present study identified a novel lncRNA associated with glioma, glioma stem-like cells (GSCs) and then revealed their potential functions. During the screening of lncRNAs, we found lncRNA RP5-821D11.7 (lncRNA-RP5) overexpress in GSCs compared to glioma cells. Lentivirus-mediated shRNA for lncRNA-RP5 was constructed and transfected into glioma cells. Transfected stable glioma cells were transplanted into nude mice and tumor growth was determined. Knockdown of lncRNA-RP5 significantly inhibits proliferation, migration and reduces epithelial-mesenchymal transition (EMT) by activating the Wnt/β-catenin pathway. Additionally, the results showed that lncRNA RP5 knockdown enhances cell apoptosis through endoplasmic reticulum stress. Therefore, this study may provide a better understanding and demonstrates that lncRNA-RP5 may be a potential therapeutic target in glioma.
{"title":"Long non-coding RNA RP5-821D11.7 promotes proliferation, migration, and epithelial-mesenchymal transition in glioma and glioma stem-like cells","authors":"","doi":"10.47262/bl/9.2.20230408","DOIUrl":"https://doi.org/10.47262/bl/9.2.20230408","url":null,"abstract":"Long noncoding RNA (lncRNA) has been recently revealed as a main regulatory molecule, implicating many cellular functions. Studies showed that lncRNA is abnormally expressed and involved in the progression and tumorigenesis of glioma. The present study identified a novel lncRNA associated with glioma, glioma stem-like cells (GSCs) and then revealed their potential functions. During the screening of lncRNAs, we found lncRNA RP5-821D11.7 (lncRNA-RP5) overexpress in GSCs compared to glioma cells. Lentivirus-mediated shRNA for lncRNA-RP5 was constructed and transfected into glioma cells. Transfected stable glioma cells were transplanted into nude mice and tumor growth was determined. Knockdown of lncRNA-RP5 significantly inhibits proliferation, migration and reduces epithelial-mesenchymal transition (EMT) by activating the Wnt/β-catenin pathway. Additionally, the results showed that lncRNA RP5 knockdown enhances cell apoptosis through endoplasmic reticulum stress. Therefore, this study may provide a better understanding and demonstrates that lncRNA-RP5 may be a potential therapeutic target in glioma.","PeriodicalId":9154,"journal":{"name":"Biomedical Letters","volume":"41 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136106988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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