Uncovering cryptochrome/photolyase gene diversity in aquatic microbiomes exposed to diverse UV-B regimes

IF 1.6 4区 环境科学与生态学 Q3 ECOLOGY Aquatic Microbial Ecology Pub Date : 2020-10-22 DOI:10.3354/ame01947
D. G. Alonso-Reyes, M. E. Farías, V. Albarracín
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引用次数: 7

Abstract

During evolution, microorganisms exposed to high amounts of UV-B irradiation developed fine-tuned photo-enzymes called ‘photolyases’ to cope with DNA damage caused by UV-B. These photoreceptors, belonging to the cryptochrome/photolyase family (CPF), have been well characterized at the genomic and proteomic level in bacteria isolated from a wide range of environments. In this work, we go further towards studying the abundance of CPF in aquatic microbial communities from different geographic regions across the globe. Metagenomics data combined with geo-referenced solar irradiation measurements indicated that the higher the UV-B level in the microbiome’s environment, the higher the abundance of CPF genes and lower the microbial diversity. A connection between CPF abundance and radiation intensity/photoperiod was found. Likewise, cryptochrome-like genes were found to be abundant in most exposed microbiomes, indicating a complementary role to standard photolyases. We observed that CPFs are more likely to be present in dominant taxa of the highly irradiated microbiomes, suggesting an evolutionary force for survival and dominance under extreme solar exposure. This work reports 3 novel CPF clades, proving the potential of global metagenomic analyses in detecting novel proteins.
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揭示暴露于不同UV-B环境下水生微生物群的隐色素/光解酶基因多样性
在进化过程中,暴露于大量UV-B照射下的微生物产生了微调的光酶,称为“光解酶”,以应对UV-B引起的DNA损伤。这些光感受器属于隐色素/光解酶家族(CPF),已经在广泛环境中分离的细菌中在基因组和蛋白质组学水平上得到了很好的表征。在这项工作中,我们将进一步研究全球不同地理区域水生微生物群落中CPF的丰度。宏基因组学数据结合地理参考太阳辐照测量结果表明,微生物组环境中UV-B水平越高,CPF基因的丰度越高,微生物多样性越低。CPF丰度与辐射强度/光周期呈正相关。同样,隐色素样基因在大多数暴露的微生物组中被发现丰富,表明与标准光解酶具有互补作用。我们观察到CPFs更有可能存在于高辐射微生物组的优势分类群中,这表明在极端阳光照射下生存和优势的进化力量。这项工作报道了3个新的CPF分支,证明了全球宏基因组分析在检测新蛋白质方面的潜力。
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来源期刊
Aquatic Microbial Ecology
Aquatic Microbial Ecology 环境科学-海洋与淡水生物学
CiteScore
3.30
自引率
0.00%
发文量
8
审稿时长
3.0 months
期刊介绍: AME is international and interdisciplinary. It presents rigorously refereed and carefully selected Research Articles, Reviews and Notes, as well as Comments/Reply Comments (for details see AME 27:209), Opinion Pieces (previously called ''As I See It'') and AME Specials. For details consult the Guidelines for Authors. Papers may be concerned with: Tolerances and responses of microorganisms to variations in abiotic and biotic components of their environment; microbial life under extreme environmental conditions (climate, temperature, pressure, osmolarity, redox, etc.). Role of aquatic microorganisms in the production, transformation and decomposition of organic matter; flow patterns of energy and matter as these pass through microorganisms; population dynamics; trophic interrelationships; modelling, both theoretical and via computer simulation, of individual microorganisms and microbial populations; biodiversity. Absorption and transformation of inorganic material; synthesis and transformation of organic material (autotrophic and heterotrophic); non-genetic and genetic adaptation; behaviour; molecular microbial ecology; symbioses.
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