ABSTRACT: Seawater microorganisms impact ecological and biogeochemical cycling on coral reefs and are sensitive indicators of ecosystem status. Microbialization, a shift towards trophic collapse and resultant high microbial biomass, is a global concern on coral reefs. Indeed, macroorganisms can influence microbial processes and community composition on reefs, which is best understood as increased macroalgae resulting in copiotrophic microbial growth and oxygen reduction. Whether or not smaller-scale changes in macroorganisms influence the overlying seawater microbial communities is largely unknown. Here, we assessed seawater microorganisms across 3 coral reefs to understand their connection to reef site and within-reef benthic characteristics. At 3 coral reefs in St. John, US Virgin Islands, we collected 60 ml seawater samples 2 cm above the seafloor, spaced 2 m apart in a grid pattern, and assessed bacterial and archaeal communities via sequencing of small subunit ribosomal RNA genes. Benthic cover within 1 m of each sample was determined at 10 cm resolution through photogrammetry. Our results reveal that overall reef site overwhelmingly shapes microbial community structure, while within-reef benthic cover surrounding sample locations has minimal influence. However, ecospheres as areas that reflect the small-scale effects of benthic cover directly under each sample, significantly explain as much as 12.1% of within-reef microbial variation and may even outweigh variation attributable to reef site alone. These findings provide new insights into fine-scale spatial variability in reef seawater microbiomes that are crucial for the use of microorganisms as indicators of microbialization and coral reef health.
{"title":"Near-benthic coral reef picoplankton vary at fine scales decoupled from benthic cover","authors":"Austin Greene, Yogesh Girdhar, Amy Apprill","doi":"10.3354/ame02011","DOIUrl":"https://doi.org/10.3354/ame02011","url":null,"abstract":"ABSTRACT: Seawater microorganisms impact ecological and biogeochemical cycling on coral reefs and are sensitive indicators of ecosystem status. Microbialization, a shift towards trophic collapse and resultant high microbial biomass, is a global concern on coral reefs. Indeed, macroorganisms can influence microbial processes and community composition on reefs, which is best understood as increased macroalgae resulting in copiotrophic microbial growth and oxygen reduction. Whether or not smaller-scale changes in macroorganisms influence the overlying seawater microbial communities is largely unknown. Here, we assessed seawater microorganisms across 3 coral reefs to understand their connection to reef site and within-reef benthic characteristics. At 3 coral reefs in St. John, US Virgin Islands, we collected 60 ml seawater samples 2 cm above the seafloor, spaced 2 m apart in a grid pattern, and assessed bacterial and archaeal communities via sequencing of small subunit ribosomal RNA genes. Benthic cover within 1 m of each sample was determined at 10 cm resolution through photogrammetry. Our results reveal that overall reef site overwhelmingly shapes microbial community structure, while within-reef benthic cover surrounding sample locations has minimal influence. However, ecospheres as areas that reflect the small-scale effects of benthic cover directly under each sample, significantly explain as much as 12.1% of within-reef microbial variation and may even outweigh variation attributable to reef site alone. These findings provide new insights into fine-scale spatial variability in reef seawater microbiomes that are crucial for the use of microorganisms as indicators of microbialization and coral reef health.","PeriodicalId":8112,"journal":{"name":"Aquatic Microbial Ecology","volume":"26 1","pages":""},"PeriodicalIF":1.4,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142210031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Georgie E. Humphries, Mariapaola Ambrosone, Zabdiel Roldan-Ayala, Maximillian Brown, Dianne I. Greenfield
ABSTRACT: A growing body of literature has highlighted the importance of phytoplankton-bacterial associations to marine and estuarine ecological and biogeochemical function, but their population linkages remain sparsely characterized within urban estuaries. Since many developed coastlines are heavily impacted by anthropogenic nutrient inputs, elucidating their phytoplankton-bacterial dynamics provides insight into nutrient cycling, productivity, and can help inform water quality management. This study compared surface (0.5 m depth) physical water quality, cell abundances of major phytoplankton taxa and bacteria, as well as concentrations of chlorophyll a (chl a) and dissolved organic matter (DOM) in the nitrogen (N)-enriched Western Long Island Sound (WLIS), USA, between mid-channel and shore sites (in 2020 and 2021). Shore bacterial and phytoplankton abundances as well as DOM concentrations (primarily dissolved organic N and carbon [DOC]), were significantly higher than mid-channel, especially during summer, indicative of terrestrial loading influencing microbial assemblages as well as N and C cycling. Abundances of key phytoplankton taxa were better indicators of bacterial abundances than chl a, as bacterial abundances positively and significantly correlated with those of dinoflagellates, especially the most common genera Prorocentrum (mid-channel, shore) and Heterocapsa (shore only), but not with diatoms. However, pennate diatom abundances negatively and significantly correlated with DOC concentrations in the mid-channel. Results highlight the impact of terrestrial inputs on WLIS microbial assemblage dynamics, presumably by favoring bacteria and dinoflagellate population coupling, as well as shed new ecological insight into how phytoplankton and bacterial communities respond to nutrient loadings in urban estuaries.
摘要：越来越多的文献强调了浮游植物与细菌的关联对海洋和河口生态及生物地球化学功能的重要性，但在城市河口，它们的种群联系仍然很少被描述。由于许多发达海岸线受到人为营养物质输入的严重影响，阐明其浮游植物-细菌动态有助于深入了解营养物质循环和生产力，并有助于为水质管理提供信息。本研究比较了美国氮（N）富集的西长岛湾（WLIS）中河道和海岸站点（2020 年和 2021 年）的表层（0.5 米深）物理水质、主要浮游植物类群和细菌的细胞丰度以及叶绿素 a (chl a) 和溶解有机物 (DOM) 的浓度。海岸细菌和浮游植物丰度以及 DOM 浓度（主要是溶解有机氮和碳 [DOC]）明显高于河道中段，尤其是在夏季，这表明陆地负荷对微生物群落以及氮和碳循环产生了影响。与叶绿素 a 相比，主要浮游植物类群的丰度是细菌丰度的更好指标，因为细菌丰度与甲藻丰度呈显著正相关，尤其是最常见的 Prorocentrum 属（河道中部、沿岸）和 Heterocapsa 属（仅沿岸），但与硅藻不相关。不过，笔形硅藻的丰度与中游河道的 DOC 浓度呈显著负相关。研究结果突显了陆地输入对 WLIS 微生物群落动态的影响（可能是通过促进细菌和甲藻的种群耦合），并对浮游植物和细菌群落如何应对城市河口的营养负荷提出了新的生态见解。
{"title":"Shore and mid-channel surveys reveal distinct phytoplankton-bacterial population associations along an urban estuary","authors":"Georgie E. Humphries, Mariapaola Ambrosone, Zabdiel Roldan-Ayala, Maximillian Brown, Dianne I. Greenfield","doi":"10.3354/ame02012","DOIUrl":"https://doi.org/10.3354/ame02012","url":null,"abstract":"ABSTRACT: A growing body of literature has highlighted the importance of phytoplankton-bacterial associations to marine and estuarine ecological and biogeochemical function, but their population linkages remain sparsely characterized within urban estuaries. Since many developed coastlines are heavily impacted by anthropogenic nutrient inputs, elucidating their phytoplankton-bacterial dynamics provides insight into nutrient cycling, productivity, and can help inform water quality management. This study compared surface (0.5 m depth) physical water quality, cell abundances of major phytoplankton taxa and bacteria, as well as concentrations of chlorophyll <i>a</i> (chl <i>a</i>) and dissolved organic matter (DOM) in the nitrogen (N)-enriched Western Long Island Sound (WLIS), USA, between mid-channel and shore sites (in 2020 and 2021). Shore bacterial and phytoplankton abundances as well as DOM concentrations (primarily dissolved organic N and carbon [DOC]), were significantly higher than mid-channel, especially during summer, indicative of terrestrial loading influencing microbial assemblages as well as N and C cycling. Abundances of key phytoplankton taxa were better indicators of bacterial abundances than chl <i>a</i>, as bacterial abundances positively and significantly correlated with those of dinoflagellates, especially the most common genera <i>Prorocentrum</i> (mid-channel, shore) and <i>Heterocapsa</i> (shore only), but not with diatoms. However, pennate diatom abundances negatively and significantly correlated with DOC concentrations in the mid-channel. Results highlight the impact of terrestrial inputs on WLIS microbial assemblage dynamics, presumably by favoring bacteria and dinoflagellate population coupling, as well as shed new ecological insight into how phytoplankton and bacterial communities respond to nutrient loadings in urban estuaries.","PeriodicalId":8112,"journal":{"name":"Aquatic Microbial Ecology","volume":"259 1","pages":""},"PeriodicalIF":1.4,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142210027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ABSTRACT: Cryptophytes are eukaryotic microalgae found in a variety of aquatic environments, from tea-colored ponds and lakes to the blue-water open ocean. To broaden the range of their spectral absorption beyond the limits of chlorophyll a, cryptophytes contain phycobiliprotein (PBP) accessory pigments. Hemiselmis pacifica contains the PBP cryptophyte-phycocyanin 577 (Cr-PC 577), which allows it to absorb green to orange wavelengths of light. Here, we characterized variability in PBP absorbance and growth rates of H. pacifica when this species was grown in nutrient-rich environments of differing spectral quality but equal light intensity. Two weeks after a shift from white to green light, H. pacifica altered the absorbance of its Cr-PC 577 to increase capture of green photons. Further, these complementary shifts were reversible when cultures were returned to the white-light environment, and the timescale of the reversal was faster than the original shift (~1 wk). Growth rates of H. pacifica in green light (0.25 d-1) were lower than in white-light controls (0.32 d-1), but not significantly different from cells grown in red light (0.27 d-1). The ability to adjust quickly to changes in light quality may confer an ecological advantage to cryptophytes when their environment is affected by processes such as eutrophication, deforestation/afforestation, or browning.
{"title":"Complementary chromatic acclimation by shifts in phycobiliprotein spectral absorption in the cryptophyte Hemiselmis pacifica","authors":"Ian D. Jin, Tammi L. Richardson","doi":"10.3354/ame02010","DOIUrl":"https://doi.org/10.3354/ame02010","url":null,"abstract":"ABSTRACT: Cryptophytes are eukaryotic microalgae found in a variety of aquatic environments, from tea-colored ponds and lakes to the blue-water open ocean. To broaden the range of their spectral absorption beyond the limits of chlorophyll <i>a</i>, cryptophytes contain phycobiliprotein (PBP) accessory pigments. <i>Hemiselmis pacifica</i> contains the PBP cryptophyte-phycocyanin 577 (Cr-PC 577), which allows it to absorb green to orange wavelengths of light. Here, we characterized variability in PBP absorbance and growth rates of <i>H. pacifica</i> when this species was grown in nutrient-rich environments of differing spectral quality but equal light intensity. Two weeks after a shift from white to green light, <i>H. pacifica</i> altered the absorbance of its Cr-PC 577 to increase capture of green photons. Further, these complementary shifts were reversible when cultures were returned to the white-light environment, and the timescale of the reversal was faster than the original shift (~1 wk). Growth rates of <i>H. pacifica</i> in green light (0.25 d<sup>-1</sup>) were lower than in white-light controls (0.32 d<sup>-1</sup>), but not significantly different from cells grown in red light (0.27 d<sup>-1</sup>). The ability to adjust quickly to changes in light quality may confer an ecological advantage to cryptophytes when their environment is affected by processes such as eutrophication, deforestation/afforestation, or browning.","PeriodicalId":8112,"journal":{"name":"Aquatic Microbial Ecology","volume":"33 1","pages":""},"PeriodicalIF":1.4,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142210028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
ABSTRACT: With a ubiquitous presence in marine ecosystems, Labyrinthulomycetes protists (LP) play critical ecological roles in oceanic habitats. Recently, some LP strains have been suggested to survive in low-salinity environments, but their distribution in freshwaters was largely unknown. This study investigated LP abundance and diversity dynamics along a fresh-saltwater gradient in 2 seasons. LP were detected in all samples. Although LP abundance in freshwaters (typically 104 to 105 copies l-1) was significantly lower than that in saline waters, their abundance still corresponded to that of previously reported LP in some coastal waters, suggesting their potentially essential roles in riverine ecosystems. High-throughput sequencing analyses identified 110, 54, and 146 LP amplicon sequence variants (ASVs) in fresh, brackish, and saline waters, respectively. Canonical correspondence analysis and variance partitioning analysis further indicated that salinity and temperature were the most significant environmental factors to affect LP community structure. Notably, most of the dominant ASVs in fresh/brackish waters were annotated to a rarely reported Labyrinthulida family, Amphitraemidae, and a newly identified river cluster of the order Thraustochytrida, which were significantly different from those of saline waters. Finally, the metabolic capabilities of the detected LP genera suggest that LP likely play diverse ecological roles in riverine ecosystems.
{"title":"Salinity gradient differentiates potential novel ecotypes and diversity of Labyrinthulomycetes protists along the Haihe River, northern China","authors":"Mohan Bai, Jiaqian Li, Xueyan Ding, Xiuping Liu, Yaodong He, Guangyi Wang","doi":"10.3354/ame02009","DOIUrl":"https://doi.org/10.3354/ame02009","url":null,"abstract":"ABSTRACT: With a ubiquitous presence in marine ecosystems, Labyrinthulomycetes protists (LP) play critical ecological roles in oceanic habitats. Recently, some LP strains have been suggested to survive in low-salinity environments, but their distribution in freshwaters was largely unknown. This study investigated LP abundance and diversity dynamics along a fresh-saltwater gradient in 2 seasons. LP were detected in all samples. Although LP abundance in freshwaters (typically 10<sup>4</sup> to 10<sup>5</sup> copies l<sup>-1</sup>) was significantly lower than that in saline waters, their abundance still corresponded to that of previously reported LP in some coastal waters, suggesting their potentially essential roles in riverine ecosystems. High-throughput sequencing analyses identified 110, 54, and 146 LP amplicon sequence variants (ASVs) in fresh, brackish, and saline waters, respectively. Canonical correspondence analysis and variance partitioning analysis further indicated that salinity and temperature were the most significant environmental factors to affect LP community structure. Notably, most of the dominant ASVs in fresh/brackish waters were annotated to a rarely reported Labyrinthulida family, Amphitraemidae, and a newly identified river cluster of the order Thraustochytrida, which were significantly different from those of saline waters. Finally, the metabolic capabilities of the detected LP genera suggest that LP likely play diverse ecological roles in riverine ecosystems.","PeriodicalId":8112,"journal":{"name":"Aquatic Microbial Ecology","volume":"8 1","pages":""},"PeriodicalIF":1.4,"publicationDate":"2024-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141503577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Karine Felix Ribeiro, Ng Haig They, Marla Sonaira Lima, Michele Bertoni Mann, Ana Paula Guedes Frazzon, Jeverson Frazzon, Leandro Duarte, Luciane Oliveira Crossetti
ABSTRACT: We explored the ecological drivers of β-diversity patterns in bacterial assemblages sharing a same ecosystem but from different habitats (planktonic and periphytic) and belonging to different functional groups (bacteria and the subgroup cyanobacteria). Assemblages were characterized based on the 16S rRNA gene in a subtropical lake system comprising 5 lakes at a maximum distance of 50 km. We measured the influence of environmental heterogeneity and geographic distance (as a proxy of dispersal) on β-diversity and its components (species replacement and species loss). Bacterial membership clearly differed between planktonic and periphytic assemblages, with most operational taxonomical units being exclusive to a single habitat. Species replacement was the major component explaining the β-diversity patterns of bacteria regardless of habitat and functional group, which was mainly influenced by environmental heterogeneity in all cases. Moreover, when compared to planktonic ones, periphytic assemblages presented higher species replacement rates with geographic distance. In conclusion, our results highlight species sorting as the major driver for bacteria and the subgroup cyanobacteria in both habitat types, with a minor influence of the dispersal limitation for periphytic assemblages, which can be explained due to their substrate-attached trait.
{"title":"Species sorting as the major driver of turnover for both planktonic and periphytic bacteria and the subgroup cyanobacteria in a subtropical lake system","authors":"Karine Felix Ribeiro, Ng Haig They, Marla Sonaira Lima, Michele Bertoni Mann, Ana Paula Guedes Frazzon, Jeverson Frazzon, Leandro Duarte, Luciane Oliveira Crossetti","doi":"10.3354/ame02008","DOIUrl":"https://doi.org/10.3354/ame02008","url":null,"abstract":"ABSTRACT: We explored the ecological drivers of β-diversity patterns in bacterial assemblages sharing a same ecosystem but from different habitats (planktonic and periphytic) and belonging to different functional groups (bacteria and the subgroup cyanobacteria). Assemblages were characterized based on the <i>16S rRNA</i> gene in a subtropical lake system comprising 5 lakes at a maximum distance of 50 km. We measured the influence of environmental heterogeneity and geographic distance (as a proxy of dispersal) on β-diversity and its components (species replacement and species loss). Bacterial membership clearly differed between planktonic and periphytic assemblages, with most operational taxonomical units being exclusive to a single habitat. Species replacement was the major component explaining the β-diversity patterns of bacteria regardless of habitat and functional group, which was mainly influenced by environmental heterogeneity in all cases. Moreover, when compared to planktonic ones, periphytic assemblages presented higher species replacement rates with geographic distance. In conclusion, our results highlight species sorting as the major driver for bacteria and the subgroup cyanobacteria in both habitat types, with a minor influence of the dispersal limitation for periphytic assemblages, which can be explained due to their substrate-attached trait.","PeriodicalId":8112,"journal":{"name":"Aquatic Microbial Ecology","volume":"64 1","pages":""},"PeriodicalIF":1.4,"publicationDate":"2024-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141197917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Linxue Qi, Xiuhong Liu, Yongqing Gao, Qing Yang, Ziheng Wang, Nan Zhang, Xueying Su
ABSTRACT: Microalgal-bacterial symbiotic wastewater treatment systems (MBSWTSs) have received widespread attention due to their capacity to achieve high pollutant removal efficiency during wastewater treatment, with low energy consumption requirements, efficient carbon sequestration, and wastewater resource utilization. This paper provides an overview of the treatment performance and current research status of MBSWTSs, including a detailed summary of the mechanisms of nitrogen, phosphorus, and carbon removal by MBSWTSs and the interactions between bacteria and microalgae. In particular, this review focuses on the influence of operational parameters on the regulation of the symbiotic system, such as the microalgal:bacterial ratio, N:P ratio, external carbon source, dissolved oxygen concentration, aeration mode, and light availability. Among these factors, the microalgal:bacterial ratio, carbon source, and light availability have an important influence on microalgal-bacterial competition, as well as the trophic mode of the system, biomass production, and the capacity for the process to be practically applied on a large scale. MBSWTSs still have some challenging aspects that have hindered their development and application, such as the unknown mechanism of microalgal-bacterial co-metabolism, limited previous practical applications, algal contamination, and harvesting difficulties. To overcome these challenges, future research requires a multidisciplinary approach, incorporating life sciences, material science, and other disciplines. Comprehensive research should be conducted on the metabolic mechanisms of MBSWTSs, the optimization of process performance and waste resource utilization, providing a theoretical and practical foundation for the practical application of MBSWTSs.
{"title":"Interactions between bacteria and microalgae in microalgal-bacterial symbiotic wastewater treatment systems: mechanisms and influencing factors","authors":"Linxue Qi, Xiuhong Liu, Yongqing Gao, Qing Yang, Ziheng Wang, Nan Zhang, Xueying Su","doi":"10.3354/ame02007","DOIUrl":"https://doi.org/10.3354/ame02007","url":null,"abstract":"ABSTRACT: Microalgal-bacterial symbiotic wastewater treatment systems (MBSWTSs) have received widespread attention due to their capacity to achieve high pollutant removal efficiency during wastewater treatment, with low energy consumption requirements, efficient carbon sequestration, and wastewater resource utilization. This paper provides an overview of the treatment performance and current research status of MBSWTSs, including a detailed summary of the mechanisms of nitrogen, phosphorus, and carbon removal by MBSWTSs and the interactions between bacteria and microalgae. In particular, this review focuses on the influence of operational parameters on the regulation of the symbiotic system, such as the microalgal:bacterial ratio, N:P ratio, external carbon source, dissolved oxygen concentration, aeration mode, and light availability. Among these factors, the microalgal:bacterial ratio, carbon source, and light availability have an important influence on microalgal-bacterial competition, as well as the trophic mode of the system, biomass production, and the capacity for the process to be practically applied on a large scale. MBSWTSs still have some challenging aspects that have hindered their development and application, such as the unknown mechanism of microalgal-bacterial co-metabolism, limited previous practical applications, algal contamination, and harvesting difficulties. To overcome these challenges, future research requires a multidisciplinary approach, incorporating life sciences, material science, and other disciplines. Comprehensive research should be conducted on the metabolic mechanisms of MBSWTSs, the optimization of process performance and waste resource utilization, providing a theoretical and practical foundation for the practical application of MBSWTSs.","PeriodicalId":8112,"journal":{"name":"Aquatic Microbial Ecology","volume":"14 1","pages":""},"PeriodicalIF":1.4,"publicationDate":"2024-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140585344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
EL Harvey, H Yang, E Castiblanco, M Coolahan, G Dallmeyer-Drennen, N Fukuda, E Greene, M Gonsalves, S Smith, KE Whalen
Viruses that infect phytoplankton are abundant in all regions of the global ocean. Despite their ubiquity, little is understood regarding how biotic interactions can alter virus infection success as well as the fate of phytoplankton hosts. In previous work, the bacterially derived compound 2-heptyl-4-quinolone (HHQ) has been shown to protect the cosmopolitan coccolithophore Emiliania huxleyi from virus-induced mortality. The present study explores the potential mechanisms through which protection is conferred. Using a suite of transmission electron microscopy and physiological diagnostic staining techniques, we show that when E. huxleyi is exposed to HHQ, viruses can gain entry into cells but viral replication and release is inhibited. These findings are supported by a smaller burst size, as well as lower infectious and total virus production when the host is treated with nanomolar concentrations of HHQ. Additionally, diagnostic staining results indicate that programmed cell death markers commonly associated with viral infection are not activated when infected E. huxleyi are exposed to HHQ. Together, these results suggest that the ability of HHQ to inhibit infectious viral production protects the alga not from getting infected, but from cell lysis. This work identifies a new mechanistic role of bacterial quorum sensing molecules in mediating viral infections in marine microbial systems.
{"title":"Quorum sensing signal disrupts viral infection dynamics in the coccolithophore Emiliania huxleyi","authors":"EL Harvey, H Yang, E Castiblanco, M Coolahan, G Dallmeyer-Drennen, N Fukuda, E Greene, M Gonsalves, S Smith, KE Whalen","doi":"10.3354/ame01998","DOIUrl":"https://doi.org/10.3354/ame01998","url":null,"abstract":"Viruses that infect phytoplankton are abundant in all regions of the global ocean. Despite their ubiquity, little is understood regarding how biotic interactions can alter virus infection success as well as the fate of phytoplankton hosts. In previous work, the bacterially derived compound 2-heptyl-4-quinolone (HHQ) has been shown to protect the cosmopolitan coccolithophore Emiliania huxleyi from virus-induced mortality. The present study explores the potential mechanisms through which protection is conferred. Using a suite of transmission electron microscopy and physiological diagnostic staining techniques, we show that when E. huxleyi is exposed to HHQ, viruses can gain entry into cells but viral replication and release is inhibited. These findings are supported by a smaller burst size, as well as lower infectious and total virus production when the host is treated with nanomolar concentrations of HHQ. Additionally, diagnostic staining results indicate that programmed cell death markers commonly associated with viral infection are not activated when infected E. huxleyi are exposed to HHQ. Together, these results suggest that the ability of HHQ to inhibit infectious viral production protects the alga not from getting infected, but from cell lysis. This work identifies a new mechanistic role of bacterial quorum sensing molecules in mediating viral infections in marine microbial systems.","PeriodicalId":8112,"journal":{"name":"Aquatic Microbial Ecology","volume":"70 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135673197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
HC Petersen, R. Sapkota, AL Hiillos, BW Hansen, GT Banta, KE Knott
{"title":"Spatial and temporal dynamics of coastal benthic microbial communities along a salinity gradient","authors":"HC Petersen, R. Sapkota, AL Hiillos, BW Hansen, GT Banta, KE Knott","doi":"10.3354/ame02002","DOIUrl":"https://doi.org/10.3354/ame02002","url":null,"abstract":"","PeriodicalId":8112,"journal":{"name":"Aquatic Microbial Ecology","volume":"79 1","pages":""},"PeriodicalIF":1.4,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78694495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
F. Yan, J. Wang, J. Dong, Y. Zhu, J. Yan, J. Zhang, H. Lu, Z. Yu, G. Xu
{"title":"Compositional responses of aquatic bacterial communities and their network interactions to phytoplankton-derived dissolved organic matter from freshwater ponds","authors":"F. Yan, J. Wang, J. Dong, Y. Zhu, J. Yan, J. Zhang, H. Lu, Z. Yu, G. Xu","doi":"10.3354/ame01997","DOIUrl":"https://doi.org/10.3354/ame01997","url":null,"abstract":"","PeriodicalId":8112,"journal":{"name":"Aquatic Microbial Ecology","volume":"13 2 1","pages":""},"PeriodicalIF":1.4,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83973937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Li, Z. Li, H. Peng, L. Yu, M. Wang, Z. Shen, Q. Gu
{"title":"Distinct bacterial communities associated with photosynthetic picoeukaryotes in Lake Dongting throughout the seasonal cycle as revealed by flow cytometry sorting","authors":"S. Li, Z. Li, H. Peng, L. Yu, M. Wang, Z. Shen, Q. Gu","doi":"10.3354/ame02004","DOIUrl":"https://doi.org/10.3354/ame02004","url":null,"abstract":"","PeriodicalId":8112,"journal":{"name":"Aquatic Microbial Ecology","volume":"27 1","pages":""},"PeriodicalIF":1.4,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84091533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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