In vitro propagation of endemic plant Centaurea arifolia Boiss. Taxa

E. Yüzbaşıoğlu, E. Dalyan, M. Bona, G. Öz
{"title":"In vitro propagation of endemic plant Centaurea arifolia Boiss. Taxa","authors":"E. Yüzbaşıoğlu, E. Dalyan, M. Bona, G. Öz","doi":"10.18478/IUFSJB.73052","DOIUrl":null,"url":null,"abstract":"Centaurea L. (Asteraceae) is one of the largest genera in the flora of Turkey. Centaurea arifolia belonging \nto the genus Centaurea is considered as DD (Data Deficient) according to IUCN category. There are \nno studies identified aiming to propagate this species in vitro. In this study, the seeds were germinated \naseptically in Petri dishes containing growth regulator free Murashige and Skoog half-strength (MS ½) \nmedium. Leaf explants of six-week-old grown seedlings were cultured on MS medium supplemented \nwith 1 mg l−1 BAP plus 0.1 mg l−1 NAA and 2 mg l−1 BAP plus 0.2 mg l−1 NAA for indirect regeneration \nand after three weeks, actively growing calli appeared and proliferated. Induction of adventitious \nshoot regeneration from calli was achieved approximately 150% (MS1) and 120% (MS2), within three \nweeks. Regenerated adventitious shoots were best rooted on MS medium containing 1 mg l−1 IBA. This \nprotocol provides a successful propagation technique through indirect in vitro organogenesis from leaf \nsegments of C. arifolia. \nKeywords: C. arifolia, endemic species, micropropagation","PeriodicalId":14521,"journal":{"name":"IUFS Journal of Biology","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2012-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"4","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"IUFS Journal of Biology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.18478/IUFSJB.73052","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 4

Abstract

Centaurea L. (Asteraceae) is one of the largest genera in the flora of Turkey. Centaurea arifolia belonging to the genus Centaurea is considered as DD (Data Deficient) according to IUCN category. There are no studies identified aiming to propagate this species in vitro. In this study, the seeds were germinated aseptically in Petri dishes containing growth regulator free Murashige and Skoog half-strength (MS ½) medium. Leaf explants of six-week-old grown seedlings were cultured on MS medium supplemented with 1 mg l−1 BAP plus 0.1 mg l−1 NAA and 2 mg l−1 BAP plus 0.2 mg l−1 NAA for indirect regeneration and after three weeks, actively growing calli appeared and proliferated. Induction of adventitious shoot regeneration from calli was achieved approximately 150% (MS1) and 120% (MS2), within three weeks. Regenerated adventitious shoots were best rooted on MS medium containing 1 mg l−1 IBA. This protocol provides a successful propagation technique through indirect in vitro organogenesis from leaf segments of C. arifolia. Keywords: C. arifolia, endemic species, micropropagation
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
地方性植物半毛菊的离体繁殖。分类单元
半人马属(菊科)是土耳其植物区系中最大的属之一。根据国际自然保护联盟(IUCN)的分类,半人马属植物被认为是DD(数据不足)。目前还没有发现旨在体外繁殖该物种的研究。在这项研究中,种子在含有不含生长调节剂的Murashige和Skoog半强度(MS½)培养基的培养皿中无菌发芽。将6周大的幼苗叶片外植体分别培养在MS培养基上,分别添加1 mg l′1 BAP + 0.1 mg l′1 NAA和2 mg l′1 BAP + 0.2 mg l′1 NAA,进行间接再生,3周后出现活跃生长的愈伤组织并增殖。在3周内,愈伤组织不定芽的诱导率分别达到150% (MS1)和120% (MS2)。再生不定芽在含有1 mg l′1 IBA的MS培养基上生根效果最好。该方案提供了一种间接离体器官发生的繁殖技术。关键词:金针叶;特有种;微繁
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
自引率
0.00%
发文量
0
期刊最新文献
Lipoxygenase Inhibitory Activities of Some Plant Extracts and Chemical Compounds Three wheat cultivars’ responses to NaCl treatments: changes in lipid peroxidation, cell viability, hydrogen peroxide content and antioxidant defence parameters The role of Escherichia coli bacterioferritin in stressed induced conditions Phenolic composition and antibacterial activity of crude methanolic Calendula officinalis flower extract against plant pathogenic bacteria Scale Surface Microstructure and Scale Size in Three Mugilid Fishes (Teleostei, Mugilidae) of Iran from Three Different Habitats
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1