Structural Characterization of Amadori Rearrangement Product of Glucosylated Nα-Acetyl-Lysine by Nuclear Magnetic Resonance Spectroscopy

Chuanjiang Li, Hui Wang, M. Juárez, E. Ruan
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引用次数: 11

Abstract

Maillard reaction is a nonenzymatic reaction between reducing sugars and free amino acid moieties, which is known as one of the most important modifications in food science. It is essential to characterize the structure of Amadori rearrangement products (ARPs) formed in the early stage of Maillard reaction. In the present study, the Nα-acetyl-lysine-glucose model had been successfully set up to produce ARP, Nα-acetyl-lysine-glucose. After HPLC purification, ARP had been identified by ESI-MS with intense [M
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糖基化n - α-乙酰赖氨酸Amadori重排产物的核磁共振谱结构表征
美拉德反应是还原糖和游离氨基酸之间的一种非酶促反应,是食品科学中最重要的修饰之一。对美拉德反应早期形成的Amadori重排产物(ARPs)的结构进行表征是十分必要的。在本研究中,成功建立了n - α-乙酰赖氨酸-葡萄糖生成ARP模型,n - α-乙酰赖氨酸-葡萄糖。高效液相色谱纯化后,用ESI-MS对ARP进行鉴定
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