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Synthesis and Characterization of Cobalt(III) and Copper(II) Complexes of 2-((E)-(6-Fluorobenzo[d]thiazol-2-ylimino) methyl)-4-chlorophenol: DNA Binding and Nuclease Studies—SOD and Antimicrobial Activities 钴(III)和铜(II)配合物2-((E)-(6-氟苯并[d]噻唑-2-ylimino)甲基)-4-氯苯酚的合成和表征:DNA结合和核酸酶研究- sod和抗菌活性
Pub Date : 2018-03-01 DOI: 10.1155/2018/8759372
K. Savithri, B. C. V. Kumar, H. K. Vivek, H. D. Revanasiddappa
A bidentate (N- and O-) imine-based ligand (L1) and its metal complexes of types [ (L1)2] (C1), [ (L1)(Phen)] (C2), [ (L1)2] (C3), and [ (L1)(Phen)] (C4) (L1 = 2-((E)-(6-fluorobenzo[d]thiazol-2-ylimino)methyl)-4-chlorophenol and phen = 1,10-phenanthroline) were synthesized as potential chemotherapeutic drug candidates. The prepared complexes were structurally characterized by spectral techniques (NMR, FT-IR, LC-MS, EPR, and electronic absorption), thermogravimetric analysis (TGA/DTA), magnetic moment, and CHNO elemental analysis. Spectroscopic studies suggested the distorted octahedral structure for all complexes. In vitro bioassay studies include binding and nuclease activities of the ligand and its complexes with target calf thymus- (CT-) DNA were carried out by employing UV-Vis, fluorescence spectroscopy, viscosity, and gel electrophoresis techniques. The extent of binding propensity was determined quantitatively by and values which revealed a higher binding affinity for C2 and C4 as compared to C1 and C3. In addition, the scavenging superoxide anion free radical ( ) activity of metal complexes was determined by nitroblue tetrazolium (NBT) light reduction assay. Molecular docking studies with DNA and SOD enzyme were also carried out on these compounds. The antimicrobial study has shown that all the compounds are potential antibacterial agents against Gram-negative bacterial strains and better antifungal agents with respect to standard drugs used.
合成了一种双齿(N-和O-)亚胺基配体(L1)及其金属配合物[(L1)2] (C1)、[(L1)(Phen)] (C2)、[(L1)2] (C3)和[(L1)(Phen)] (C4) (L1 = 2-((E)-(6-氟苯并[d]噻唑-2-ylimino)甲基)-4-氯苯酚和Phen = 1,10-菲罗啉)作为潜在的化疗候选药物。通过NMR、FT-IR、LC-MS、EPR和电子吸收等波谱技术、热重分析(TGA/DTA)、磁矩分析和CHNO元素分析对所制备的配合物进行了结构表征。光谱研究表明,所有配合物都具有扭曲的八面体结构。体外生物测定研究包括配体及其复合物与靶小牛胸腺(CT-) DNA的结合和核酸酶活性,采用UV-Vis、荧光光谱、粘度和凝胶电泳技术进行。与C1和C3相比,对C2和C4具有更高的结合亲和力,从而定量地确定了结合倾向的程度。此外,采用硝基蓝四氮唑(NBT)光还原法测定了金属配合物清除超氧阴离子自由基的活性。对这些化合物进行了与DNA和SOD酶的分子对接研究。抗菌研究表明,所有化合物都是潜在的抗革兰氏阴性菌株的抗菌药物,与标准药物相比,是更好的抗真菌药物。
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引用次数: 22
Application of Electrochemical Techniques on Study of Effect of Nano-ZnO in Conductive Polyaniline Containing Zinc-Rich Primer 电化学技术在纳米zno导电聚苯胺富锌底漆中的应用研究
Pub Date : 2018-02-28 DOI: 10.1155/2018/7160381
Ximing Li, H. Castaneda
Effect of zinc oxide nanoparticles on anticorrosion performance has been studied in conductive polyaniline containing zinc-rich primer in 3.5 wt% NaCl solution, using Electrochemical Impedance Spectroscopy (EIS) and localized electrochemical Scanning Vibrating Electrode Technique (SVET). The results showed that the addition of nano-zinc oxide particles in conductive polyaniline containing zinc-rich primer made the reaction of zinc more stable and slower, further increasing the effective cathodic protection period. EIS and SVET results confirmed that three performance evolution stages were obtained for zinc-rich primer being immersed in 3.5 wt% sodium chloride solution.
采用电化学阻抗谱(EIS)和局部电化学扫描振动电极技术(SVET)研究了氧化锌纳米颗粒对含锌导电聚苯胺底漆在3.5 wt% NaCl溶液中防腐性能的影响。结果表明,在含锌的导电聚苯胺底漆中加入纳米氧化锌颗粒,使锌的反应更加稳定和缓慢,进一步提高了有效阴极保护期。EIS和SVET结果证实,富锌引物浸泡在3.5 wt%氯化钠溶液中有3个性能演化阶段。
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引用次数: 5
New Ni-Anthracene Complex for Selective and Sensitive Detection of 2,4,6-Trinitrophenol 新型ni -蒽配合物选择性灵敏检测2,4,6-三硝基苯酚
Pub Date : 2018-02-06 DOI: 10.1155/2018/1321427
Kumbam Lingeshwar Reddy, Anabathula Manoj Kumar, Abhimanew Dhir, V. Krishnan
Selective and sensitive detection of explosive materials through a simple approach is an attractive area of research having implications on public safety and homeland security. Considering this implication in mind, a new Ni-anthracene complex was designed and synthesized and has been demonstrated as an efficient fluorescence chemosensor for the selective and sensitive detection of 2,4,6-trinitrophenol. Firstly, a fluorescent anthracene ligand (A) was synthesized by treating anthracene-9-carboxaldehyde with 1,3-diaminopropane in presence of a weak acid. To achieve superior selectivity and great quenching efficiency for 2,4,6-trinitrophenol (TNP), a Ni complex, namely, [Ni( -L)(NO3)] (B), was synthesized via the reaction of A with Ni(NO3)2·6H2O. Complex B showed strong emission peak ( ) at 412 nm and exhibited high selectivity towards TNP among other nitroaromatics and anions. 100 equivalents of TNP made 95% fluorescence quenching of B and its detection limit for TNP was calculated as 2.8 μM.
通过一种简单的方法对爆炸性材料进行选择性和灵敏的探测是一个具有吸引力的研究领域,对公共安全和国土安全具有影响。考虑到这一意义,设计并合成了一种新的ni -蒽配合物,并已被证明是一种高效的荧光化学传感器,可选择性和灵敏地检测2,4,6-三硝基苯酚。首先,用1,3-二氨基丙烷在弱酸存在下处理蒽-9-甲醛,合成了荧光蒽配体(a)。为获得2,4,6-三硝基苯酚(TNP)的高选择性和高猝灭效率,通过a与Ni(NO3)2·6H2O反应合成了一种Ni配合物[Ni(-L)(NO3)] (B)。配合物B在412 nm处表现出较强的发射峰(),在其他硝基芳烃和阴离子中对TNP具有较高的选择性。100当量的TNP可使B的荧光猝灭95%,其对TNP的检出限为2.8 μM。
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引用次数: 5
Application of Autofluorescence for Analysis of Medicinal Plants 自体荧光技术在药用植物分析中的应用
Pub Date : 2017-11-26 DOI: 10.1155/2017/7159609
V. V. Roshchina, A. Kuchin, V. Yashin
Autofluorescence of secondary compounds contained in plant secretory cells may be applied to the analysis of medicinal plants for pharmacy. Emission and prevailing fluorescent pharmaceuticals have been estimated in several models of species such as Salvia officinalis, Berberis vulgaris, Humulus lupulus, and Matricaria chamomilla, by luminescence microscopy, microspectrofluorimetry, and confocal microscopy.
植物分泌细胞中二级化合物的自体荧光分析可用于药用植物的药学分析。利用发光显微镜、显微荧光法和共聚焦显微镜,对几种物种模型(如鼠尾草、小檗、葎草和甘菊)的发光和流行荧光药物进行了估计。
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引用次数: 17
Fourier Spectroscopy: A Bayesian Way 傅立叶光谱学:贝叶斯方法
Pub Date : 2017-10-31 DOI: 10.1155/2017/9265084
S. Schmuck, J. Svensson
The concepts of standard analysis techniques applied in the field of Fourier spectroscopy treat fundamental aspects insufficiently. For example, the spectra to be inferred are influenced by the noise contribution to the interferometric data, by nonprobed spatial domains which are linked to Fourier coefficients above a certain order, by the spectral limits which are in general not given by the Nyquist assumptions, and by additional parameters of the problem at hand like the zero-path difference. To consider these fundamentals, a probabilistic approach based on Bayes’ theorem is introduced which exploits multivariate normal distributions. For the example application, we model the spectra by the Gaussian process of a Brownian bridge stated by a prior covariance. The spectra themselves are represented by a number of parameters which map linearly to the data domain. The posterior for these linear parameters is analytically obtained, and the marginalisation over these parameters is trivial. This allows the straightforward investigation of the posterior for the involved nonlinear parameters, like the zero-path difference location and the spectral limits, and hyperparameters, like the scaling of the Gaussian process. With respect to the linear problem, this can be interpreted as an implementation of Ockham’s razor principle.
傅立叶光谱领域中应用的标准分析技术的概念对基本方面的处理不够。例如,要推断的光谱受到以下因素的影响:噪声对干涉数据的贡献、与傅里叶系数在一定阶以上相关联的未探测空间域、奈奎斯特假设通常没有给出的光谱极限、以及手头问题的附加参数(如零径差)。为了考虑这些基本原理,介绍了一种基于贝叶斯定理的概率方法,该方法利用多元正态分布。对于实例应用,我们用一个先验协方差表示的布朗桥的高斯过程来模拟谱。光谱本身由若干线性映射到数据域的参数表示。这些线性参数的后验是解析得到的,这些参数的边缘是微不足道的。这允许对涉及的非线性参数(如零路径差位置和光谱限制)和超参数(如高斯过程的缩放)的后验进行直接调查。对于线性问题,这可以解释为奥卡姆剃刀原理的实现。
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引用次数: 2
Annular Beam Shaping in Multiphoton Microscopy to Reduce Out-of-Focus Background 环形光束整形在多光子显微镜减少失焦背景
Pub Date : 2017-05-24 DOI: 10.1155/2017/7560141
Johan Borglin, Danni Wang, N. Durr, D. Hanstorp, A. Ben-Yakar, M. Ericson
Despite the inherent spatial confinement of multiphoton processes that arises from focusing through an objective, the maximum imaging depth in conventional multiphoton microscopy is ultimately limited by noise from out-of-focus fluorescence. This is particularly evident when imaging beyond shallow depths in highly scattering tissue as increased laser powers are necessary. The out-of-focus signal originates from multiphoton processes taking place primarily at shallow depths and deteriorates contrast and limits imaging depth. In this paper, annular laser beams are explored as a concept to reduce this background signal in multiphoton microscopy. The approach is theoretically verified by data from simulations and proof of principle is demonstrated on a custom-built experimental multiphoton microscopy platform. Annular laser beams were created by adopting wavefront control using a spatial light modulator and implemented for imaging tissue phantoms simulating turbid media and human skin ex vivo. The signal-to-background ratios were calculated and compared to images acquired with a traditional, filled-aperture Gaussian beam. Experiments in tissue phantom show an improvement in signal-to-background ratio of about 30% when using annular beam illumination in comparison to Gaussian illumination at specific depths. When laser power is not the limiting factor, this approach is expected to provide even greater benefits.
尽管通过物镜聚焦产生的多光子过程的固有空间限制,但传统多光子显微镜的最大成像深度最终受到失焦荧光噪声的限制。这是特别明显的,当成像超过浅深度的高散射组织,增加激光功率是必要的。失焦信号源于主要发生在浅深度的多光子过程,它降低了对比度并限制了成像深度。本文探讨了在多光子显微镜中,环形激光束作为一种减小背景信号的概念。该方法在理论上得到了仿真数据的验证,并在一个定制的实验多光子显微镜平台上进行了原理验证。利用空间光调制器采用波前控制产生环形激光束,实现了模拟浑浊介质和人体皮肤的组织幻象离体成像。计算了信号背景比,并与传统的填充孔径高斯光束获得的图像进行了比较。实验表明,在特定深度下,环形光束照明比高斯照明可提高30%左右的信本比。当激光功率不是限制因素时,这种方法有望提供更大的好处。
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引用次数: 4
Nile Red and 2-NBDG Are Incompatible for the Simultaneous Detection of Lipid and Glucose Accumulation 尼罗红与2-NBDG在同时检测脂质和葡萄糖积累时不相容
Pub Date : 2016-12-22 DOI: 10.1155/2016/5215086
A. M. Hogan, Viswanathan Swaminathan, Nikitha K. Pallegar, S. L. Christian
Glucose is the universal energy source and a critical substrate for lipid synthesis in mammalian cells. Analysis of both glucose and lipid in cells is important for the understanding of the regulation of lipid synthesis in many cell types, but especially adipocytes, the major storage cell for fat in mammals. The fluorescent 7-nitrobenz-2-oxa-1,3-diazole (NBD) derivative of glucose, 2-NBDG, is used to monitor glucose uptake and the lipid-selective fluorophore Nile red is used to monitor lipid accumulation. Previous reports have used NBD-based fluorophores and Nile red simultaneously despite the possibility of spectral overlap. In this study, we determined if these fluorophores were experimentally compatible in preadipocytes and adipocytes stained with 2-NBDG and Nile red separately or costained. We found that Nile red is detectable in the wavelengths necessary to excite and detect 2-NBDG. This interference was further increased by the solvatochromic effect of lipid-localized Nile red. In addition, we found a synergistic increase in fluorescent intensity when both fluorophores were present. Unfortunately, even fine control of the excitation or emission wavelengths did not identify wavelengths suitable for selective detection when cells were costained. Therefore, 2-NBDG and Nile red cannot be used simultaneously—but can likely be used sequentially—to assess glucose uptake and lipid accumulation in lipid-laden cells.
葡萄糖是哺乳动物细胞中普遍存在的能量来源和脂质合成的关键底物。分析细胞中的葡萄糖和脂质对于理解许多细胞类型中脂质合成的调节非常重要,尤其是脂肪细胞,哺乳动物脂肪的主要储存细胞。葡萄糖的7-硝基苯-2-氧杂-1,3-二唑(NBD)荧光衍生物2-NBDG用于监测葡萄糖摄取,脂质选择性荧光团尼罗红用于监测脂质积累。以前的报告同时使用了nbd基荧光团和尼罗红,尽管光谱可能重叠。在这项研究中,我们确定了这些荧光团在脂肪前细胞和2-NBDG和尼罗红分别染色或染色的脂肪细胞中是否实验相容。我们发现尼罗河红在激发和探测2-NBDG所需的波长中是可探测的。脂质定域尼罗红的溶剂化变色作用进一步增加了这种干扰。此外,我们发现当两种荧光团存在时,荧光强度协同增加。不幸的是,当细胞染色时,即使对激发或发射波长进行精细控制,也不能确定适合选择性检测的波长。因此,2-NBDG和尼罗红不能同时使用,但可以依次使用,以评估脂质细胞中的葡萄糖摄取和脂质积累。
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引用次数: 3
Quantum Chemical Calculations and Molecular Docking Studies of Some NSAID Drugs (Aceclofenac, Salicylic Acid, and Piroxicam) as 1PGE Inhibitors 一些非甾体抗炎药(醋氯芬酸、水杨酸和吡罗昔康)作为1PGE抑制剂的量子化学计算和分子对接研究
Pub Date : 2016-09-29 DOI: 10.1155/2016/5281636
S. Suresh, Sethu Gunasekaran, Shanmugam Srinivasan
The molecular structure of the three compounds Aceclofenac (I), Salicylic Acid (II), and Piroxicam (III) has been determined using Gaussian 03W program with B3LYP method using 6-311++G (d,p) basis set calculations. The molecular structures were fully optimized with atomic numbering scheme adopted in the study. To understand the mode of binding and molecular interaction, the docking studies of compounds Aceclofenac (I), Salicylic Acid (II), and Piroxicam (III) have been carried out with prostaglandin H2 synthase-1 (1PGE) as target using induced fit docking. The molecular docking results show that the interactions and energy for Aceclofenac, Salicylic Acid, and Piroxicam show the best results when docked with prostaglandin H2 synthase-1 (1PGE). The hydrogen bonding interactions of compound I (Aceclofenac) are prominent with Arginine moiety, those of compound II (Salicylic Acid) are prominent with Tyrosine and Serine moieties, and compound III (Piroxicam) shows such interaction with Tyrosine and Arginine moieties. These interactions of prostaglandin H2 synthase-1 (1PGE) with substrates are responsible for governing COX-1 inhibitor potency which in turn is a direct measure of the potency of the drug.
采用高斯03W程序和B3LYP方法,采用6-311++G (d,p)基集计算,确定了Aceclofenac (I)、水杨酸(II)和吡罗昔康(III)三种化合物的分子结构。采用原子序数方案对分子结构进行了充分优化。为了了解它们的结合模式和分子相互作用,我们以前列腺素H2合成酶-1 (1PGE)为靶点,采用诱导配合对接的方法对化合物Aceclofenac (I)、水杨酸(II)和吡罗昔康(III)进行对接研究。分子对接结果表明,当与前列腺素H2合成酶-1 (prostaglandin H2 synthase-1, 1PGE)对接时,乙酰氯芬酸、水杨酸和吡罗昔康的相互作用和能量表现最佳。化合物I(乙酰氯芬酸)与精氨酸部分的氢键相互作用突出,化合物II(水杨酸)与酪氨酸和丝氨酸部分的氢键相互作用突出,化合物III(吡罗昔康)与酪氨酸和精氨酸部分的氢键相互作用突出。前列腺素H2合成酶-1 (1PGE)与底物的这些相互作用负责控制COX-1抑制剂的效力,这反过来又是药物效力的直接测量。
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引用次数: 4
Determination of Alcohol Content in Alcoholic Beverages Using 45 MHz Benchtop NMR Spectrometer 45兆赫台式核磁共振光谱仪测定酒精饮料中的酒精含量
Pub Date : 2016-08-17 DOI: 10.1155/2016/2526946
Meden F. Isaac-Lam
Alcohol or ethanol is considered the most widely used recreational drug worldwide, and its production, consumption, and sale are strictly regulated by laws. Alcohol content of alcoholic beverages (wine, beers, and spirits) is about 3–50% v/v. Analytical methods to determine the alcohol content must be reliable, precise, and accurate. In this study, the amount of ethanol in several alcoholic beverages was determined using a 45 MHz low-field benchtop NMR (nuclear magnetic resonance) spectrometer. Internal standard and standard addition analytical methods were utilized to quantify ethanol. For both methods, acetic acid or acetonitrile was used as internal standard to quantify alcohol content by using the peak area corresponding to the methyl peaks of ethanol, acetic acid, or acetonitrile. Results showed that internal standard method gave values of percent alcohol that are in close agreement with the indicated label as confirmed by running the samples in a 400 MHz high-field NMR spectrometer using acetic acid as internal standard. This study demonstrates the utility of a benchtop NMR spectrometer that can provide an alternative technique to analyze percent alcohol in alcoholic products.
酒精或乙醇被认为是世界上使用最广泛的娱乐性毒品,它的生产、消费和销售都受到法律的严格管制。酒精饮料(葡萄酒、啤酒和烈酒)的酒精含量约为3-50% v/v。测定酒精含量的分析方法必须可靠、精确和准确。在这项研究中,使用45 MHz低场台式核磁共振光谱仪测定了几种酒精饮料中的乙醇含量。采用内标法和标准添加法对乙醇进行定量分析。两种方法均以乙酸或乙腈为内标,利用乙醇、乙酸或乙腈甲基峰对应的峰面积定量乙醇含量。结果表明,用400 MHz高场核磁共振波谱仪以乙酸为内标,内标法测得的酒精含量与标示值基本一致。本研究证明了台式核磁共振光谱仪的实用性,它可以提供一种替代技术来分析酒精产品中的酒精含量。
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引用次数: 39
Atomic Structure Calculations for Neutral Oxygen 中性氧的原子结构计算
Pub Date : 2016-05-25 DOI: 10.1155/2016/1697561
N. Alonizan, R. Qindeel, N. B. Nessib
Energy levels and oscillator strengths for neutral oxygen have been calculated using the Cowan (CW), SUPERSTRUCTURE (SS), and AUTOSTRUCTURE (AS) atomic structure codes. The results obtained with these atomic codes have been compared with MCHF calculations and experimental values from the National Institute of Standards and Technology (NIST) database.
用Cowan (CW)、SUPERSTRUCTURE (SS)和AUTOSTRUCTURE (AS)原子结构代码计算了中性氧的能级和振子强度。用这些原子代码得到的结果已与MCHF计算和美国国家标准与技术研究所(NIST)数据库中的实验值进行了比较。
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引用次数: 3
期刊
International Journal of Spectroscopy
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