Production of Cellulase by Aspergillus niger on natural and pretreated lignocellulosic wastes

Abstract

Growth of Aspergillus niger on Czapek-dox medium supplemented with native lignocellulosics like Sawdust, wheat straw, Sugarcane baggage and Ricebran used for the production of cellulase by A.niger. Of these Czapek-dox medium with 0.5% Wheat straw had yielded more cellulase, FPase (2.9 U/ml), CMCase (2.9 U/ml) and β-glucosidase (0.93 U/ml) after 14-days of incubation. The lignocellulosics after pre-treatment improved cellulase production. Among the treated substrates, Czapek-dox medium with 0.5% saw dust had yielded FPase (6.3 U/ml), CMCase (7.2 U/ml) after 7-days of incubation and the medium with treated rice bran produced β-glucosidase of 1.04 U/ml after 21-days of incubation.