ANTICANCER EFFECT OF AgTOEPyP4 PORPHYRIN

Proceedings of the YSU B: Chemical and Biological Sciences Pub Date : 2023-03-27 DOI:10.46991/pysu:b/2023.57.1.041

N. Karapetyan, G. Ananyan, S. Haroutiunian

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Abstract

The silver-containing porphyrin Ag-meso-tetra(4-N-hydroxyethylpyridyl)porphyrin (AgTOEPyP4) was investigated as a potential anticancer drug using spectrophotometric, calorimetric and electrophoretic measurements. The studies were conducted on healthy, cancer-induced, and treated by AgTOEPyP4 white mice. DNA was isolated from the liver and tumor tissues of mice. Melting curves were studied in the presence of various stoichiometric concentrations of Mn2+ ions. The character of the DNA–Mn2+ interaction in the tumor differs from that in the norm due to defects in the secondary structure of tumor DNA, which is expressed in the DNA melting characteristics of DNA. It was revealed that the melting parameters, enthalpy, and electrophoretic mobility of DNA isolated from the tumor-bearing mice tissues differed from healthy DNA. It was shown that all studied parameters of the DNA isolated from the liver and tumor treated by AgTOEPyP4 mice approached the norm. The obtained results revealed that AgTOEPyP4 porphyrin has a pronounced therapeutic effect against sarcoma S180 and requires further investigation as an anticancer drug.

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AgTOEPyP4卟啉的抗癌作用

采用分光光度法、量热法和电泳法研究了含银卟啉ag -中四(4- n -羟乙基吡啶基)卟啉(AgTOEPyP4)作为一种潜在的抗癌药物。这些研究是在健康、癌症诱导和AgTOEPyP4治疗的小白鼠身上进行的。从小鼠的肝脏和肿瘤组织中分离DNA。研究了不同化学计量浓度的Mn2+离子存在下的熔化曲线。肿瘤中DNA - mn2 +相互作用的特点不同于正常情况，这是由于肿瘤DNA的二级结构存在缺陷，表现为DNA的DNA熔化特性。结果表明，从荷瘤小鼠组织中分离的DNA的熔化参数、焓和电泳迁移率与健康DNA不同。结果表明，经AgTOEPyP4处理的小鼠肝脏和肿瘤中分离的DNA的所有研究参数均接近正常值。结果表明，AgTOEPyP4卟啉对S180肉瘤具有明显的治疗作用，作为一种抗癌药物有待进一步研究。

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Proceedings of the YSU B: Chemical and Biological Sciences

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