A review on chemistry, therapeutic applications, extraction & purification of bromelain

Abstract

This review focused on the usage of bromelain in numerous applications, as well as the most recent literature on the chemistry, extraction, and purification of bromelain from pineapple (Ananas comosus). Bromelain is a crude, aqueous extract obtained from the stems and fruits of pineapple, leading edible member of the family Bromeliaceae. Bromelain contains a mixture of different thiol endopeptidases as well as phosphatases, glucosidases, peroxidases, cellulases, & glycoproteins. It exerts its enzymatic activity over a PH range 4.5 – 9.5. The isolation & purification of bromelain has been done by various methods includes aqueous two-phase extraction, reverse micellar extraction, ultrafiltration & precipitation & chromatographic techniques. Bromelain, a cysteine protease, has found commercial use in a variety of industries including food, beverage, tenderization, cosmetic, pharmaceutical, and textile. Bromelain act as an immunomodulatory, anti-inflammatory agent, anti-oedematous, anti-thrombotic & anti-metastatic. Although the precise mechanism of action of bromelain has not been established, bromelain has received widespread acceptance as a phytotherapeutic agent due to its long history of safety and absence of adverse effects