The Analysis of Pork Content in Processed Beef Meatballs at Ketahun, North Bengkulu District Using Genetic Marker Mitochondrial DNA Cytochrome b

Abstract

This research aims to analyze the content of pork in the sold meatballs in the North Bengkulu district. The study was conducted in December 2019-May 2020. Meatball samples were taken from 12 meatball merchant stalls in Ketahun, North Bengkulu district. DNA genome samples were isolated from the meatball using DNeasy guidelines and protocols® mericon® Food Qiagen Mini Kit. The Cyt b gene of mitochondrial DNA was amplified using forward universal primer, reverse cow primer, reverse pig primer, and reverse mixed primer PCR (Polymerase Chain Reaction) as much as 30 cycles. The amplification result was running on electrophoresis with agarose gel 1% and visualized with UV Transluminator Gel Document System Axygen. The data obtained were then analyzed. The DNA band of each sample was visualized on gel agarose compared to marker 1 Kb with a size of 398 bp (pig content marker). The results of the study showed that there were five of twelve meatball samples were contaminated with pork, namely BU7, BU8, BU9, BU11, and BU12. The information of pork content on meatballs can be used as a determining material in food safety policy in Bengkulu Province.