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The Effectiveness of Piper betel L. Leaf Extract as a Natural Electric Mosquito 槟榔叶提取物作为天然电蚊的效果研究
S. Safrida, F. A. Ulhusna
Mosquitoes are known as insects that interfere with comfort. Mosquitoes are also known as vectors or transmitters of several types of dangerous and deadly diseases for humans, one of which is dengue fever. Therefore, an alternative is needed to reduce this species. The alternative that can be done is by providing vegetable insecticides. Betel leaf has the potential to be used as a base for making plant-based insecticides that are environmentally friendly because they contain chemical compounds in the form of phenols and derivatives such as chavikol and eugenol, alkaloids, tannins, flavonoids, saponins and essential oils. This study aims to determine the concentration of betel leaf extract (Piper betel L.) which is effective for killing mosquitoes. This study used an experimental method using a completely randomized design (CRD) with 6 treatments and 4 replications, namely KN as negative control group, KP as control positive (100% HIT brand liquid), P1 25% extract, P2 50% extract, P3 75% extract, and P4 100% extract. The number of dead mosquitoes (mortality) were observed after 1 hour of treatment. Data were analyzed using variance analysis (ANAVA). The results of this study were the administration of various concentrations of betel leaf extract (Piper betel L.) significantly (p <0.05) to kill mosquitoes. The greatest frequency of mosquito mortality was found at the P4, the highest extract concentration. The conclusion of this study is that betel leaf extract for 1 hour had a significant effect on mosquito mortality.
蚊子被认为是妨碍舒适的昆虫。蚊子还被认为是几种人类危险和致命疾病的媒介或传播者,其中之一是登革热。因此,需要一种替代方法来减少这种物种。另一种方法是提供蔬菜杀虫剂。槟榔叶有可能被用作制造对环境友好的植物性杀虫剂的基础,因为它们含有酚类化合物和衍生物,如沙维醇和丁香酚、生物碱、单宁、类黄酮、皂苷和精油。本研究旨在确定槟榔叶提取物(Piper betel L.)的杀蚊效果浓度。本研究采用完全随机设计(CRD)的试验方法,共6个处理,4个重复,即KN为阴性对照组,KP为对照阳性组(100% HIT品牌液),P1为25%提取物组,P2为50%提取物组,P3为75%提取物组,P4为100%提取物组。处理1 h后观察蚊虫死亡数(死亡率)。数据采用方差分析(ANAVA)进行分析。结果表明,不同浓度槟榔叶提取物(Piper betel L.)对蚊虫的杀伤效果显著(p <0.05)。在提取液浓度最高的P4处,蚊虫死亡率最高。本研究的结论是,槟榔叶提取物作用1小时对蚊虫死亡率有显著影响。
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引用次数: 1
The Phenotype Characters Stability of Hybrid Watermelon (Citrullus lanatus (Thunb.) Matsum. & Nakai) ♀ ‘Maduri’ and ♂ ‘Putri Delima’ 杂交西瓜(Citrullus lanatus (Thunb.))表型性状稳定性研究Matsum。& Nakai♀' Maduri '和♂' Putri Delima '
D. Sartika, A. Irsyad, Rizal Hermawan S. Budi, Wahyu Dwi Soemantri, B. Daryono
Watermelon (Citrullus lanatus (Thunb.) Matsum. & Nakai) is one of the horticultural crops members of Cucurbitaceae family, which is known to have high economic value and public interest. Since the entry into Indonesia, the application of watermelon plant breeding has grown to produce new superior varieties, hence not only able to meet market demand, but also to have implications for the progress of the agribusiness sector and national food security. Watermelon ♀ ‘Maduri’ with ♂ ‘Putri Delima’ cultivar hybrid produce an oblong-shaped fruit, has a dark green skin tone with dark green streaks, orange flesh fruit, and sweet taste. However, to be certified and become a cultivar that can be cultivated extensively, the new varieties need to have an adaptive and stable character. This study aims to determine the phenotype character and stability of watermelon ♀ ‘Maduri’ with ♂ ‘Putri Delima’ cultivar hybrid which is cultivated in Jamusan Village, Prambanan Sub-district, Sleman, D.I.Yogyakarta fields. Furthermore, 1 randomly collected watermelon samples were taken. Then, the qualitative and quantitative characters of samples were observed. The quantitative data were analyzed using Microsoft Excel 2016 with ANOVA single factor test at 1% and 5% level. The result of ANOVA test at 5% significance level shows F value at 0,00055 and Fcrit value at 4,259677273, while at the l% significance level, F value obtained at 0,00055 and the Fcrit value obtained at 7,822871. F value is smaller than Fcrit value at 5% and 1% significance level so there is no significance difference. Thus, the phenotype character of F2 watermelon is deemed stable.
西瓜(Citrullus lanatus)Matsum。中葵(Nakai)是葫芦科园艺作物成员之一,具有很高的经济价值和公众利益。自进入印度尼西亚以来,西瓜植物育种的应用已经发展到生产新的优良品种,因此不仅能够满足市场需求,而且对农业综合企业部门的进步和国家粮食安全产生影响。西瓜♀‘Maduri’与♂‘Putri Delima’杂交品种产生椭圆形的果实,具有深绿色的皮肤色调和深绿色的条纹,橙色的果肉,味道甜美。然而,新品种要获得认证并成为可广泛栽培的品种,需要具有适应性和稳定性。本研究以日惹市Sleman市Prambanan街道Jamusan村西瓜为材料,对西瓜♀‘Maduri’与♂‘Putri Delima’杂交品种的表型性状和稳定性进行了研究。随机抽取1个西瓜样品。然后,观察样品的定性和定量特征。定量数据采用Microsoft Excel 2016进行单因素方差分析,在1%和5%水平下进行单因素检验。5%显著性水平下的方差分析结果显示,F值为0,00055,Fcrit值为4,259677273,而在1%显著性水平下,F值为0,00055,Fcrit值为7,822871。在5%和1%显著性水平下,F值小于Fcrit值,无显著性差异。因此,F2西瓜的表型特征是稳定的。
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引用次数: 0
Elicitor Effect of Chitosan on In Vitro Culture of Different Explants of Physalis Accessions From East Java 壳聚糖对东爪哇不同种质离体培养的激发效应
R. Mastuti, J. Batoro, Budi Waluyo
Chitosan is a natural molecule derived from arthropods and fungi that can stimulate many physiological responses in plants. Chitosan had also shown positive effect on in vitro shoot regeneration. Therefore, this research aims to observe the response of shoot growth of in vitro Physalis plant accessions obtained from several areas in Madura Island and East Java on chitosan effect. Two types of explants shoot tips and in vitro nodal explants for each Physalis plant accession were cultured on elicitation medium (MS basal medium supplemented with BAP 2 mg / l and IAA 0.05 mg /l) containing chitosan elicitor (0, 75, 125 mg /l). The number and height of shoots were observed weekly for six weeks. The results showed that both types of explants from all accessions were able to regenerate and multiply shoots however shoot tip explants showed better growth. A2 accessions from Madura Sampang was able to regenerate shoots in high a number than other accessions on chitosan concentration of 125 mg / l. These results provide a future prospect to study synthesis of secondary metabolites in Physalis accession induced by chitosan elicitors.
壳聚糖是从节肢动物和真菌中提取的天然分子,能刺激植物的多种生理反应。壳聚糖对离体芽再生也有积极作用。因此,本研究旨在观察来自马杜拉岛和东爪哇几个地区的离体Physalis植株的茎部生长对壳聚糖效应的响应。在含壳聚糖诱导剂(0、75、125 mg /l)的诱导培养基(MS基础培养基中添加BAP 2 mg /l和IAA 0.05 mg /l)上培养两种类型的芽尖外植体和离体节段外植体。每周观察芽数和芽高,连续6周。结果表明,两种外植体均能再生和繁殖,但茎尖外植体生长较好。当壳聚糖浓度为125 mg / l时,马都拉Sampang A2材料再生芽数明显高于其他材料,这为壳聚糖激发剂诱导Physalis植株次生代谢产物合成的研究提供了前景。
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引用次数: 1
Study on Maintenance and Utilization of Tamed Sumatran Elephants (Elephas maximus sumatranus Temminck 1847) at the Elephant Training Center in the Seblat TWA Area, Bengkulu Bengkulu Seblat TWA地区大象训练中心驯化苏门答腊象(elephhas maximus sumatranus Temminck 1847)的维护和利用研究
Rizwar, Darmi, C. H. Sukoco
In the range of 23 years, from 1992 to 2015, there has been a drastic decrease in the number of individual tamed Sumatran elephants (Elephas maximus sumatranus Temminck 1847) at the Seblat Elephant Training Center (PLG) from 40 individuals to 18 individuals. Decreasing the size of the tamed elephant population is suspected to be inappropriate elephant maintenance at PLG, disease, old age and malnutrition. Good governance in the maintenance and utilization of tamed elephants at PLG is thought to be able to increase the life span of elephants and their reproductive capacity. This study aims to examine the management of PLG, especially in the maintenance and utization of tamed elephants conducted by BKSDA, Bengkulu Province. The study was conducted by direct survey method to PLG Seblat and interviewing as well as searching various relevant literatures. The parameters observed refer to the minimum standard reference for handling elephants in captivity. The results of the study found that the maintenance and utilization of the tamed elephants at PLG were only partially fulfilling the minimum standard of management of the guided elephants such as training, grazing, trekking and patrolling. While the form of maintenance that is still below the standard that needs to be improved is related to the handling of elephant health and mitigation of tamed elephant conflict with communities around
从1992年到2015年的23年间,在塞布拉特大象训练中心(PLG)被驯服的苏门答腊象(Elephas maximus sumatranus Temminck 1847)的数量急剧减少,从40头减少到18头。减少被驯服大象的数量被怀疑是不适当的大象养护在PLG,疾病,老年和营养不良。人们认为,对PLG驯养大象的维护和利用进行良好的管理,能够延长大象的寿命和繁殖能力。本研究旨在考察PLG的管理,特别是在班古鲁省BKSDA进行的驯养大象的维护和利用方面。本研究采用直接调查法,对PLG Seblat进行访谈,并查阅各种相关文献。所观察到的参数是指处理圈养大象的最低标准参考。研究结果发现,驯象群的维护和利用仅部分达到了对驯象群进行训练、放牧、徒步和巡逻等最低管理标准。虽然仍低于标准的维护形式需要改进,但与处理大象健康和减轻驯服大象与周围社区的冲突有关
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引用次数: 0
In-Silico Studies of Antitumor Activity From Red Ginger (Alpinia purpurata Vieill) Bioactive Compounds 红姜(Alpinia purpurata Vieill)生物活性化合物抗肿瘤活性的计算机研究
Santika Lusia Utami, Yogi Adhi Nugroho, Luthfiana Hardianingtyas, F. Kamilah
Many plants from the genus Alpinia are known to have several bioactive compounds that can act as an antitumor in cancer treatments. However, the potency of the bioactive compounds from red ginger as an antitumor agent has not been reported before. This research aims to study the inhibitory activity of several red ginger bioactive compounds against antitumor AKT1 protein through in-silico analysis. In this study, four identified bioactive compounds namely rutin, sitosteryl 3-O-6-palmytoil-β-d-glucoside, kumatakenin, kaempferol-3-o-glucuronide were used as a ligand for molecular interactions. The 3D structure of targetted protein AKT1 (PDB ID: 3O96) was obtained from the PDB database. [6]-shogaol, an antiproliferative and AKT1 inhibitor, was used as a control. In-silico docking analysis is performed in PyRx with the AutoDock Vina program. All bioactive compounds used in this experiment demonstrated good antitumor activity by binding to the inhibitor site of AKT1 protein. Rutin displayed the best potency as an inhibitor to AKT1 with the optimum binding energy of 11,3 kcal/mol as compared to control with -7,4 kcal/mol. These results suggest that bioactive compounds from red ginger may have the potency as an antitumor and can be developed to treat cancer.
许多来自高山属的植物已知含有几种生物活性化合物,可以作为抗癌治疗的抗肿瘤药物。然而,从红姜中提取的生物活性化合物作为抗肿瘤药物的效力尚未见报道。本研究旨在通过计算机分析研究几种红姜生物活性化合物对抗肿瘤AKT1蛋白的抑制活性。本研究以芦丁、谷甾醇-3-o- 6棕榈酸-β-d-葡萄糖苷、熊竹素、山奈酚-3-o-葡萄糖苷四种已鉴定的生物活性化合物为配体进行分子相互作用。从PDB数据库中获得目标蛋白AKT1 (PDB ID: 3O96)的三维结构。[6]以抗增殖和AKT1抑制剂-shogaol作为对照。在PyRx中使用AutoDock Vina程序进行硅对接分析。本实验中使用的所有生物活性化合物均通过结合AKT1蛋白抑制位点显示出良好的抗肿瘤活性。芦丁对AKT1的抑制效果最好,其最佳结合能为11.3 kcal/mol,而对照为-7,4 kcal/mol。这些结果表明,从红姜中提取的生物活性化合物可能具有抗肿瘤的效力,并且可以开发用于治疗癌症。
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引用次数: 0
Genetic Variation of Black Capped White Eye Zosterops atricapilla (Aves: Zosteropidae) Based on Mitochondrial DNA Cytochrome B Gene 基于线粒体DNA细胞色素B基因的黑头白眼毛毛狭蝽遗传变异(目:狭蝽科
Deby Permana, Jarulis, Sipriyadi, Santi Nurul Kamilah
{"title":"Genetic Variation of Black Capped White Eye Zosterops atricapilla (Aves: Zosteropidae) Based on Mitochondrial DNA Cytochrome B Gene","authors":"Deby Permana, Jarulis, Sipriyadi, Santi Nurul Kamilah","doi":"10.2991/absr.k.210621.058","DOIUrl":"https://doi.org/10.2991/absr.k.210621.058","url":null,"abstract":"","PeriodicalId":20665,"journal":{"name":"Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86814015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Detection Contaminant DNA of a Pig on a Sample Meat Mills in Some City Markets Bengkulu, Using a Genetic Marker DNA Mitochondria Cytochrome B 利用遗传标记DNA线粒体细胞色素B检测蚌库鲁市一些城市市场肉类加工厂样品中猪的污染DNA
C. Muslim, Iin Isnaini, Sipriyadi
The purpose of the study was to find out if there was a mixture of pork in ground beef samples in some Bengkulu city markets using the molecular Genetic DNA mitochondrial cytochrome b. The research was conducted from December 2019 to May 2020. Sampling was conducted at 10 meat mills in Bengkulu City (Pasar Panorama, Pasar Minggu, Pasar Barukoto, and Sungai Serut Fish Market). DNA isolation, DNA amplification, and electrophoresis are carried out in the biotechnology laboratory, Department of Biology, University of Bengkulu. The meat samples that had been milled were then performed genome DNA isolation following the Qiagen DNeasy for Food Kit protocol. PCR (Polymerous Chain Reaction) is carried out as many as 30 cycles with Primer Forward referring to and primary Reverse Mix (pork, cow, and chicken), Reverse Pork for pigs and Reverse cow for cows. PCR products are electrophobic on 1% agarose gel and then visualized under UV light using Gel Document System Axygen. As a result of the study, on the primary use of Rcow all samples contained cow DNA. In primary use reverse pork, no DNA tape is amplified. In the primary use of Reverse mix from ten samples all containing cow DNA, one sample is thought to contain pig DNA i.e. s7 samples, and six of them contain chicken DNA (S1, S4, S5, S6, S7, and S8). Generally, meat milling in Bengkulu city located in Pasar Panorama, Pasar Minggu, Pasar Barukoto, and Sungai Serut Fish Market is not tainted with pork even though one sample is found, this is because it is influenced by the amount of meat that is milled still too much.
该研究的目的是利用分子遗传DNA线粒体细胞色素b来确定明古鲁一些城市市场的碎牛肉样本中是否含有猪肉混合物。该研究于2019年12月至2020年5月进行。在明古鲁市的10个肉类加工厂(Pasar Panorama、Pasar Minggu、Pasar Barukoto和Sungai Serut鱼市场)进行了抽样。DNA分离、DNA扩增和电泳在Bengkulu大学生物系生物技术实验室进行。将研磨后的肉类样品按照Qiagen DNA for Food Kit协议进行基因组DNA分离。PCR (Polymerous Chain Reaction)进行多达30个循环,引物正向参照和初级反向混合(猪肉、牛和鸡),猪为反向猪肉,牛为反向奶牛。PCR产物在1%琼脂糖凝胶上电泳,然后在紫外灯下使用gel Document System oxygen进行可视化。研究结果表明,在首次使用Rcow时,所有样品都含有奶牛的DNA。在主要使用反向猪肉时,没有DNA磁带被扩增。在对10个全部含有牛DNA的样本进行反向混合的初步使用中,一个样本被认为含有猪DNA,即s7个样本,其中6个含有鸡DNA (S1, S4, S5, S6, s7和S8)。一般来说,在明古鲁市,位于帕萨尔Panorama,帕萨尔Minggu,帕萨尔Barukoto和Sungai Serut鱼市场的肉类加工中,即使发现一个样本,也不会受到猪肉污染,这是因为加工的肉量仍然过多。
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引用次数: 0
α-Glucosidase and α -Amylase Inhibitory Activities of Jambolan (Syzygium cumini (L.) SKEELS) Fruit and Seed 木柱兰对α-葡萄糖苷酶和α-淀粉酶的抑制作用水果和种子
E. Ishartati, D. Roeswitawati, Sukardi, S. Rohman, Sudiadi
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引用次数: 0
The Variation of Betulinic Acid Content of Andalas Tree (Morus macroura var. Macroura) in Different Localities of West Sumatra 西苏门答腊不同地区安达拉树(Morus macrooura var. macroura)桦木酸含量的变化
N. Aseny, Erma Nur Atika, Syamsuardi, F. Ismed
Recently, herbal ingredients have been utilized as candidate supplements or drugs to increase the resistance of body against diseases caused by viruses. In general, research is focused on plants used as herbal medicine in Indonesia. Sumatran mulberry (Morus macroura var. Macroura) is the native plant tree species in West Sumatra with the local name of the Andalas tree that has the potential to be used as a medicinal plant against the viruses desease due to its betulinic acid content. The content of secondary metabolites in a plant species can vary due to the influence of various environmental factors in their habitats. This study explored the content of betulinic acid of Morus macroura var. Macroura from four different locations in West Sumatra (Tanjung Bungo, Nagari Koto Hilalang, Paninjauan and Andaleh). Sample extracted from leaves and the level of content were examined using the Densitometric Thin-layer Chromatography (TLC) methods with the pure betulinic acid commpound as a standar. In this paper, we describe the various content of betulinic acid of Andalas Tree and discuss its potentiality as source of medicinal product based on the recent references.
近年来,草药成分已被用作候选补充剂或药物,以提高人体对病毒引起的疾病的抵抗力。总的来说,研究的重点是印度尼西亚用作草药的植物。苏门答腊桑树(Morus macroura var. macroura)是西苏门答腊岛的本地植物树种,当地名称为Andalas树,由于其桦木酸含量,有可能被用作抗病毒病的药用植物。植物次生代谢物的含量会受到生境环境因素的影响而发生变化。本研究对西苏门答腊丹戎本果、长里科托希兰朗、帕尼贾万和安达利四个不同产地的桑(Morus macoura var. macoura)的桦木酸含量进行了研究。以白桦酸纯化合物为标准品,采用薄层色谱法测定其含量。本文介绍了安提拉斯桦木中桦木酸的各种含量,并结合国内外文献对其作为药用原料的潜力进行了探讨。
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引用次数: 0
Seed Micromorphology of Orthosiphon spp. and Its Relatives (Lamiaceae) 正虹吸属及其近缘科植物种子显微形态研究
Sudarmono
The genus Orthosiphon in the Lamiaceae or Mint family is closely related to Ocimum and is economically important for medicinal and ornamental plants. Even though the leaf and flower morphology look different, but the characteristics of the seeds (nutlets) are almost the same. This study aims to determine the micromorphological character of the Orthosiphon seeds using a Scanning Electron Microscope (SEM). Observation of seeds apart from using SEM also with light microscopy on four taxa of Orthosiphon spp (Orthosiphon aristatus with pink flower, O. aristatus with white flower, O. endanghidayatae sp. nov., O. radjaampatensis sp. nov. and two taxa of Ocimum (O. canum and O. basilicum) as comparisons of their close relatives in the Lamiaceae. The largest seed size in O. aristatus with purple flowers (2727 μm x 1364 μm) was elliptic oblong and the smallest size was O. hidayatae (2182 μm x 1591 μm in the oblong shape), while the color of the seeds was from blackish brown to black. A detailed explanation of the surface of the seeds, whether or not trichomes, the characters at the end of the seed axis are described in this paper. The evolution of the main seed characters in Orthosiphon is also discussed.
薄荷科的正虹吸属是酢浆草的近亲,在药用和观赏植物中具有重要的经济价值。虽然叶子和花的形态看起来不同,但种子(小坚果)的特征几乎是相同的。本研究旨在利用扫描电子显微镜(SEM)研究正虹吸种子的微观形态特征。除扫描电镜外,还利用光镜对四种正虹吸属植物(粉红花正虹吸、白花正虹吸、濒危正虹吸、radjaampatensis sp. 11)和两种正虹吸属植物(木犀和basilicum)的近缘种进行了种子观察。紫花马兜铃种子最大(2727 μm × 1364 μm)为椭圆形长圆形,最小(2182 μm × 1591 μm)为椭圆形长圆形,种子颜色介于黑褐色到黑色之间。本文对种子表面是否有毛状体、种子轴末端的性状作了详细的说明。讨论了正虹吸主要种子性状的演化。
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引用次数: 0
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Proceedings of the 3rd KOBI Congress, International and National Conferences (KOBICINC 2020)
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