UJI AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL BIJI EDAMAME (Glycin max (L) Merril) DENGAN METODE DPPH

JURNAL ILMIAH FARMASI AKADEMI FARMASI JEMBER Pub Date : 2021-01-27 DOI:10.53864/jifakfar.v1i1.8

Hadi Barru Hakam Fajar Siddiq, Rosida, Erika Fauziah Prabawati

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Abstract

Antioxidants are compounds that can inhibit oxidation reactions with the free radicals and molecules are highly reactive. According to the previous research, edamame (Glycin max (L)Merril) plant is one of the plants that contains isoflavon compound that serves as an antioxidant. This study aims to determine the antioxidant activity of edamame extracts. This study is an experimental study. Edamame beans extract obtained by remaceration during 24 hours using 96% ethanol. The concentration of edamame beans extract were make different concentration from 90 ppm, 121,3 ppm, 143 ppm, 173,1 ppm. The extract then added with 0,02 g of DPPH free radicals that have been disolved in 100 mL of 96% ethanol. Those solutions were measured at wavelength 516,5 nm by an UV-Vis spectrophotometer. Vitamin C used as positive control. The result of Antioxidant activity of edamame beans extract has IC50value of 177,2 ppm and Vitamin C as a control has a IC50value 0,18 ppm.It means that 177,2 ppm of edamame beans extract classified as low antioxidant.Keywords : edamame beans (Glycin max L Merril, Antioxidant activity, DPPH method

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用DPPH方法测试EDAMAME种子(max Glycin)的抗氧化活性和提取物

抗氧化剂是一种能抑制自由基氧化反应的化合物，具有高活性。根据以往的研究，毛豆(Glycin max (L)Merril)植物是一种含有异黄酮化合物的植物，具有抗氧化剂的作用。本研究旨在测定毛豆提取物的抗氧化活性。这项研究是一项实验性研究。用96%的乙醇再发酵24小时得到的毛豆提取物。毛豆提取物的浓度分别为90 ppm、121 ppm、3 ppm、143 ppm、173 ppm、1 ppm。然后在提取液中加入0.02 g溶解于100 mL 96%乙醇中的DPPH自由基。用紫外-可见分光光度计在波长516,5 nm处测量这些溶液。维生素C作为阳性对照。毛豆提取物的抗氧化活性ic50值为177.2 ppm，维生素C作为对照的ic50值为0.18 ppm。也就是说，毛豆提取物中的177.02 ppm属于低抗氧化剂。关键词:毛豆;抗氧化活性;DPPH法

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JURNAL ILMIAH FARMASI AKADEMI FARMASI JEMBER

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