S. Muniswamy, R. Lokesha, R. Saxena, B. Fakrudin, K. Patel, Yamanura
{"title":"Molecular dissection of genetic diversity in pigeonpea [Cajanus cajan (L) Millsp.] minicore collection","authors":"S. Muniswamy, R. Lokesha, R. Saxena, B. Fakrudin, K. Patel, Yamanura","doi":"10.18805/lr-3817","DOIUrl":null,"url":null,"abstract":"The present investigation was carried out using 191genotypes as mini core collections of pigeonpea along with 5 check varieties to know the genetic diversity at molecular level. Significant variation was observed by the way of analysis of variance for nine characters viz., days to 50% flowering, days to maturity, plant height, number of branches per plant, pod bearing length, number of pods per plant, number of seeds per pod, seed yield per plant and hundred seed weight. Molecular diversity using 18 polymorphic simple sequence repeat (SSR) markers divided genotypes into 15 clusters, of which ICP11059 and AK-101 were solitary, indicating their distinctiveness among all genotypes. Similarly, BSMR-533, JKM-7, RVK-285, ICP-1126, ICP-348, ICP-6859 and ICP-7869 were found distinct among the genotypes. Geographical origin based diversity separated Indian and non Indian genotypes. The Un weighted Pair Group Method with Arithmetic mean (UPGMA) based dendrogram indicated distinctiveness of ICP-13633 and Bennur local, as they formed solitary cluster. The SSR marker CcM 602, as it could differentiate 4 genotypes at different base pair size can be used for identification and finger printing of genotypes.","PeriodicalId":18181,"journal":{"name":"Legume Research","volume":"33 1","pages":"32-38"},"PeriodicalIF":0.8000,"publicationDate":"2018-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Legume Research","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.18805/lr-3817","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"AGRONOMY","Score":null,"Total":0}
引用次数: 1
Abstract
The present investigation was carried out using 191genotypes as mini core collections of pigeonpea along with 5 check varieties to know the genetic diversity at molecular level. Significant variation was observed by the way of analysis of variance for nine characters viz., days to 50% flowering, days to maturity, plant height, number of branches per plant, pod bearing length, number of pods per plant, number of seeds per pod, seed yield per plant and hundred seed weight. Molecular diversity using 18 polymorphic simple sequence repeat (SSR) markers divided genotypes into 15 clusters, of which ICP11059 and AK-101 were solitary, indicating their distinctiveness among all genotypes. Similarly, BSMR-533, JKM-7, RVK-285, ICP-1126, ICP-348, ICP-6859 and ICP-7869 were found distinct among the genotypes. Geographical origin based diversity separated Indian and non Indian genotypes. The Un weighted Pair Group Method with Arithmetic mean (UPGMA) based dendrogram indicated distinctiveness of ICP-13633 and Bennur local, as they formed solitary cluster. The SSR marker CcM 602, as it could differentiate 4 genotypes at different base pair size can be used for identification and finger printing of genotypes.
本研究以191个基因型和5个对照品种为研究对象,从分子水平上了解遗传多样性。通过方差分析,在开花至50%天数、成熟天数、株高、单株分枝数、荚果长、单株荚果数、单株种子数、单株种子产量和百粒重9个性状上观察到显著差异。利用18个多态简单序列重复(SSR)标记的分子多样性将基因型划分为15个簇,其中ICP11059和AK-101为孤立簇,表明它们在所有基因型中具有差异性。同样,BSMR-533、JKM-7、RVK-285、ICP-1126、ICP-348、ICP-6859和ICP-7869在基因型中存在差异。基于地理起源的多样性区分了印度和非印度基因型。基于UPGMA的非加权对群法(unweighted Pair Group Method with Arithmetic mean, UPGMA)的树形图显示,ICP-13633与Bennur局域具有独特性,因为它们形成了孤簇。SSR标记CcM 602可以区分不同碱基对大小的4种基因型,可用于基因型的鉴定和指纹图谱。
期刊介绍:
Legume Research An International Journal. The Journal is an official publication of Agricultural Research Communication Centre. It is designed to bring out the original research articles on genetics, breeding, physiology, bacterial avtivity, production, quality, biochemistry and seeds of legumes crops. The objective of the journal is to serve as a forum for scientific community to publish their research findings on legumes ans to provide basis for new research. Journal is being scanned in the important indexing and abstracting services.