Biochemical Composition and Preliminary Qualitative Analysis of Marine ClamGafrarium divaricatum (Gmelin) From Mumbai, West Coast of India

A. Eswar, K. Ramamoorthy, Z. Isha, S. Gokulakrishnan
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Abstract

The present work investigates the potential use of the whole tissue of clam Gafrarium divaricatum (Gmelin) as a food source in a coastal region of India. In order to evaluate its food value, a detailed biochemical analysis of the whole tissue has been carried out. The results of biochemical analysis show very high protein content (26.32%), Carbohydrate (11.23%) and lipid (1.29%). The protein consists of 10 essential and 9 non essential amino acids which are as follows (Lysine 14.36%, Histidine 9.02, Methionine 8.92 %) and (Alanine 5.94%, Aspartic acid 4.98, Asparagine 3.79, Tyrosine 3.52 and Proline 3.21%) are the predominant essential amino and non essential amino acids. G. divaricatum consists of 6 different fatty acids out of which 2 are saturated fatty acids (SFA), one monounsaturated fatty acids (MUFA) and three polyunsaturated fatty acids (PUFA). SFA, MUFA and PUFA content was 27.18, 11.02, 12.47, 17.96, 11.38 and 11.38% respectively in fat (1.29 gm/100gm). In addition it contains Vitamins such as Vitamin A (112.3 IU), Vitamin C (24.11 mg/g), D (13.96 IU), B12 (1.98μg/g.), E (1.14 mg/g), K (0.59 mg/g) and B6 (0.31mg/g). Mineral composition of whole tissue showed presence of Calcium 312.74 mg/g, Sodium 89.93mg/g, Magnesium 61.11 mg/g, Potassium 21.38 mg/g as major and Copper 1.43, iron 1.37 and zinc 0.38 mg/g as minor quantity. The test for secondary metabolites shows the presence of alkaloids, phenolics, terpenes, carotenoids and steroids. This shows that the clam species is a good alternative food source to fish and can be very well exploited after its toxicity evaluation. The presence of secondary metabolites will have some medicinal values like hepatoprotective activity and anti oxidant activity which is currently under investigation.
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印度西海岸孟买海产异花螯虾(Gmelin)的生化组成及初步定性分析
目前的工作调查了整个组织的潜在用途的蛤蜊Gafrarium divaricatum (Gmelin)作为食物来源在印度沿海地区。为了评价其食用价值,对整个组织进行了详细的生化分析。生化分析结果表明,其蛋白质(26.32%)、碳水化合物(11.23%)和脂肪(1.29%)含量非常高。蛋白质由10种必需氨基酸和9种非必需氨基酸组成,其中赖氨酸14.36%、组氨酸9.02、蛋氨酸8.92%,丙氨酸5.94%、天冬氨酸4.98、天冬氨酸3.79、酪氨酸3.52、脯氨酸3.21%为主要必需氨基酸和非必需氨基酸。G. divaricatum由6种不同的脂肪酸组成,其中2种是饱和脂肪酸(SFA), 1种单不饱和脂肪酸(MUFA)和3种多不饱和脂肪酸(PUFA)。脂肪中SFA、MUFA和PUFA含量分别为27.18、11.02、12.47、17.96、11.38和11.38% (1.29 gm/100gm)。此外,还含有维生素A (112.3 IU)、维生素C (24.11 mg/g)、D (13.96 IU)、B12 (1.98 mg/g)、E (1.14 mg/g)、K (0.59 mg/g)、B6 (0.31mg/g)等多种维生素。全组织矿物组成主要为钙312.74 mg/g、钠89.93mg/g、镁61.11 mg/g、钾21.38 mg/g,微量为铜1.43、铁1.37、锌0.38 mg/g。次生代谢物检测显示存在生物碱、酚类、萜烯、类胡萝卜素和类固醇。这表明,蛤是鱼类的一种很好的替代食物来源,经过毒性评价,可以很好地开发利用。次生代谢物的存在将具有一定的药用价值,如肝保护活性和抗氧化活性,目前正在研究中。
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