{"title":"Micronuclei assay as biomarker for ethyl methane sulphonate (EMS) genotoxicity in Eclipta alba (L.) Hassk.","authors":"Radha Mishra, G. Kumar","doi":"10.31742/IJGPB.80.3.11","DOIUrl":null,"url":null,"abstract":"The eukaryotic cells are complemented with a single nucleus. However, several cells show abnormal small nuclei known as micronuclei, with addition to the main nucleus. These anomalous small nuclei along with main nucleus which may be the result of exposure of cell to cytotoxic agents (Chemicals/physical mutagens). These mutagens also cause structural or numerical chromosomal aberrations. The treatment of EMS, a chemical mutagen, induces micronuclei formation and nuclear as well as chromosomal peculiarities in Eclipta alba. The effect of micronuclei is discussed in terms of cytotoxicity of genotoxins and several evidences of nuclear polymorphism illustrated due to the mutagenic effect of EMS. Pollen viability was also examined through pollen fertility. Micronuclei causes the genomic instability and elimination of genome which is effectively put to use in breeding programmes for the creation of aneuploids and also for haploid lines.","PeriodicalId":13321,"journal":{"name":"Indian Journal of Genetics and Plant Breeding","volume":null,"pages":null},"PeriodicalIF":1.0000,"publicationDate":"2020-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Indian Journal of Genetics and Plant Breeding","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.31742/IJGPB.80.3.11","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
The eukaryotic cells are complemented with a single nucleus. However, several cells show abnormal small nuclei known as micronuclei, with addition to the main nucleus. These anomalous small nuclei along with main nucleus which may be the result of exposure of cell to cytotoxic agents (Chemicals/physical mutagens). These mutagens also cause structural or numerical chromosomal aberrations. The treatment of EMS, a chemical mutagen, induces micronuclei formation and nuclear as well as chromosomal peculiarities in Eclipta alba. The effect of micronuclei is discussed in terms of cytotoxicity of genotoxins and several evidences of nuclear polymorphism illustrated due to the mutagenic effect of EMS. Pollen viability was also examined through pollen fertility. Micronuclei causes the genomic instability and elimination of genome which is effectively put to use in breeding programmes for the creation of aneuploids and also for haploid lines.
期刊介绍：
Advance the cause of genetics and plant breeding and to encourage and promote study and research in these disciplines in the service of agriculture; to disseminate the knowledge of genetics and plant breeding; provide facilities for association and conference among students of genetics and plant breeding and for encouragement of close relationship between them and those in the related sciences; advocate policies in the interest of the nation in the field of genetics and plant breeding, and facilitate international cooperation in the field of genetics and plant breeding.
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