UJI AKTIVITAS ANTIBAKTERI EKSTRAK DAUN TEMBELEKAN (Lantana camara Linn) TERHADAP PERTUMBUHAN Staphylococcus aureus dan Escherichia coli

Biocelebes Pub Date : 2022-01-13 DOI:10.22487/bioceb.v15i2.15540

Gaby Maulida Nurdin

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Abstract

This study aimed to determine the effect of concentration ethanol extract from tembelakan leaf (Lantana camara Linn) on bacteria growth of Staphylococcus aureus and Escherichia coli. Extraction was done by maceration using ethanol 96% and then separated using rotary evaporator. Antibacterial activity test of the ethanol extract by Well agar diffusion method. Variation in crude extract saponin used in this study was 5%, 10%, 15%, 20%, 25% and positive controls were used for comparison with Amoxicilin and Chloramphenicole concentration of 25 µg/mL and DMSO as a negative control. The results of antibacterial activity test is indicated by the formation of growth inhibitory region S. aureus and E. coli. The result of growth inhibitory regions was analyzed by One way ANOVA. One way ANOVA test results indicate that there are effects of ethanol extract concentration of tembelekan leaf (L. camara Linn) against S. aureus and E. coli. Effective concentration of ethanol extract tembelekan leaf (L. camara Linn) when compared with positive control to inhibit the growth of S. aureus and E. coli is at 25% with a relatively strong antibacterial activity. Test with phytochemicals screening method which is showed that tembelekan leaf contains the flavanoid, saponins, and tannins compounds as antibacterial

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对葡萄球菌菌提取物(Lantana camara Linn)生长中的抗菌活性进行测试

本研究旨在研究黄芩叶乙醇提取物对金黄色葡萄球菌和大肠杆菌生长的影响。用96%乙醇浸渍提取，再用旋转蒸发器分离。Well琼脂扩散法测定乙醇提取物的抑菌活性。本研究中使用的粗提物皂苷的变化率分别为5%、10%、15%、20%、25%，阳性对照为阿莫西林和氯霉素浓度为25µg/mL，阴性对照为DMSO。对金黄色葡萄球菌和大肠杆菌形成生长抑制区，表明其抑菌活性。生长抑制区结果采用单因素方差分析。单因素方差分析结果表明，不同浓度的乙醇提取物对金黄色葡萄球菌和大肠杆菌有一定的抑制作用。与阳性对照相比，乙醇提取物tembelekan leaf (L. camara Linn)抑制金黄色葡萄球菌和大肠杆菌生长的有效浓度为25%，具有较强的抗菌活性。用植物化学物质筛选法进行试验，结果表明坦贝兰叶含有类黄酮、皂苷和单宁等具有抗菌作用的化合物

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Biocelebes

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